[2023-03-16 23:05:03,923] [INFO] DFAST_QC pipeline started.
[2023-03-16 23:05:03,923] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 23:05:03,923] [INFO] DQC Reference Directory: /var/lib/cwl/stga864df56-68b7-45de-9ca7-773137f6f546/dqc_reference
[2023-03-16 23:05:05,150] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 23:05:05,151] [INFO] Task started: Prodigal
[2023-03-16 23:05:05,151] [INFO] Running command: cat /var/lib/cwl/stgbfddb9de-4013-4d68-af17-bc69830f4ab6/OceanDNA-b227.fa | prodigal -d OceanDNA-b227/cds.fna -a OceanDNA-b227/protein.faa -g 11 -q > /dev/null
[2023-03-16 23:05:13,973] [INFO] Task succeeded: Prodigal
[2023-03-16 23:05:13,973] [INFO] Task started: HMMsearch
[2023-03-16 23:05:13,973] [INFO] Running command: hmmsearch --tblout OceanDNA-b227/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga864df56-68b7-45de-9ca7-773137f6f546/dqc_reference/reference_markers.hmm OceanDNA-b227/protein.faa > /dev/null
[2023-03-16 23:05:14,173] [INFO] Task succeeded: HMMsearch
[2023-03-16 23:05:14,174] [INFO] Found 6/6 markers.
[2023-03-16 23:05:14,188] [INFO] Query marker FASTA was written to OceanDNA-b227/markers.fasta
[2023-03-16 23:05:14,189] [INFO] Task started: Blastn
[2023-03-16 23:05:14,189] [INFO] Running command: blastn -query OceanDNA-b227/markers.fasta -db /var/lib/cwl/stga864df56-68b7-45de-9ca7-773137f6f546/dqc_reference/reference_markers.fasta -out OceanDNA-b227/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:05:14,777] [INFO] Task succeeded: Blastn
[2023-03-16 23:05:14,778] [INFO] Selected 22 target genomes.
[2023-03-16 23:05:14,778] [INFO] Target genome list was writen to OceanDNA-b227/target_genomes.txt
[2023-03-16 23:05:14,790] [INFO] Task started: fastANI
[2023-03-16 23:05:14,790] [INFO] Running command: fastANI --query /var/lib/cwl/stgbfddb9de-4013-4d68-af17-bc69830f4ab6/OceanDNA-b227.fa --refList OceanDNA-b227/target_genomes.txt --output OceanDNA-b227/fastani_result.tsv --threads 1
[2023-03-16 23:05:28,175] [INFO] Task succeeded: fastANI
[2023-03-16 23:05:28,176] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga864df56-68b7-45de-9ca7-773137f6f546/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 23:05:28,176] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga864df56-68b7-45de-9ca7-773137f6f546/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 23:05:28,176] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 23:05:28,176] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 23:05:28,176] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 23:05:28,176] [INFO] DFAST Taxonomy check result was written to OceanDNA-b227/tc_result.tsv
[2023-03-16 23:05:28,177] [INFO] ===== Taxonomy check completed =====
[2023-03-16 23:05:28,177] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 23:05:28,177] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga864df56-68b7-45de-9ca7-773137f6f546/dqc_reference/checkm_data
[2023-03-16 23:05:28,179] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 23:05:28,183] [INFO] Task started: CheckM
[2023-03-16 23:05:28,183] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b227/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b227/checkm_input OceanDNA-b227/checkm_result
[2023-03-16 23:05:55,499] [INFO] Task succeeded: CheckM
[2023-03-16 23:05:55,499] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 84.85%
Contamintation: 0.98%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-03-16 23:05:55,501] [INFO] ===== Completeness check finished =====
[2023-03-16 23:05:55,502] [INFO] ===== Start GTDB Search =====
[2023-03-16 23:05:55,502] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b227/markers.fasta)
[2023-03-16 23:05:55,502] [INFO] Task started: Blastn
[2023-03-16 23:05:55,502] [INFO] Running command: blastn -query OceanDNA-b227/markers.fasta -db /var/lib/cwl/stga864df56-68b7-45de-9ca7-773137f6f546/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b227/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:05:56,438] [INFO] Task succeeded: Blastn
[2023-03-16 23:05:56,439] [INFO] Selected 28 target genomes.
[2023-03-16 23:05:56,439] [INFO] Target genome list was writen to OceanDNA-b227/target_genomes_gtdb.txt
[2023-03-16 23:05:57,238] [INFO] Task started: fastANI
[2023-03-16 23:05:57,238] [INFO] Running command: fastANI --query /var/lib/cwl/stgbfddb9de-4013-4d68-af17-bc69830f4ab6/OceanDNA-b227.fa --refList OceanDNA-b227/target_genomes_gtdb.txt --output OceanDNA-b227/fastani_result_gtdb.tsv --threads 1
[2023-03-16 23:06:13,207] [INFO] Task succeeded: fastANI
[2023-03-16 23:06:13,209] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 23:06:13,209] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_012963185.1	s__DTWD01 sp012963185	80.8577	233	441	d__Bacteria;p__Acidobacteriota;c__Vicinamibacteria;o__Vicinamibacterales;f__UBA8438;g__DTWD01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 23:06:13,209] [INFO] GTDB search result was written to OceanDNA-b227/result_gtdb.tsv
[2023-03-16 23:06:13,209] [INFO] ===== GTDB Search completed =====
[2023-03-16 23:06:13,210] [INFO] DFAST_QC result json was written to OceanDNA-b227/dqc_result.json
[2023-03-16 23:06:13,210] [INFO] DFAST_QC completed!
[2023-03-16 23:06:13,210] [INFO] Total running time: 0h1m9s