[2023-03-19 03:50:07,836] [INFO] DFAST_QC pipeline started.
[2023-03-19 03:50:07,836] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 03:50:07,836] [INFO] DQC Reference Directory: /var/lib/cwl/stg3398f1b5-0ae0-44d0-9c75-4b4955f82b21/dqc_reference
[2023-03-19 03:50:09,605] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 03:50:09,605] [INFO] Task started: Prodigal
[2023-03-19 03:50:09,605] [INFO] Running command: cat /var/lib/cwl/stg517f2889-1f7b-42fe-a3c1-a292136d415b/OceanDNA-b23388.fa | prodigal -d OceanDNA-b23388/cds.fna -a OceanDNA-b23388/protein.faa -g 11 -q > /dev/null
[2023-03-19 03:50:23,539] [INFO] Task succeeded: Prodigal
[2023-03-19 03:50:23,539] [INFO] Task started: HMMsearch
[2023-03-19 03:50:23,539] [INFO] Running command: hmmsearch --tblout OceanDNA-b23388/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3398f1b5-0ae0-44d0-9c75-4b4955f82b21/dqc_reference/reference_markers.hmm OceanDNA-b23388/protein.faa > /dev/null
[2023-03-19 03:50:23,775] [INFO] Task succeeded: HMMsearch
[2023-03-19 03:50:23,776] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg517f2889-1f7b-42fe-a3c1-a292136d415b/OceanDNA-b23388.fa]
[2023-03-19 03:50:23,793] [INFO] Query marker FASTA was written to OceanDNA-b23388/markers.fasta
[2023-03-19 03:50:23,794] [INFO] Task started: Blastn
[2023-03-19 03:50:23,794] [INFO] Running command: blastn -query OceanDNA-b23388/markers.fasta -db /var/lib/cwl/stg3398f1b5-0ae0-44d0-9c75-4b4955f82b21/dqc_reference/reference_markers.fasta -out OceanDNA-b23388/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 03:50:24,442] [INFO] Task succeeded: Blastn
[2023-03-19 03:50:24,443] [INFO] Selected 11 target genomes.
[2023-03-19 03:50:24,444] [INFO] Target genome list was writen to OceanDNA-b23388/target_genomes.txt
[2023-03-19 03:50:24,449] [INFO] Task started: fastANI
[2023-03-19 03:50:24,449] [INFO] Running command: fastANI --query /var/lib/cwl/stg517f2889-1f7b-42fe-a3c1-a292136d415b/OceanDNA-b23388.fa --refList OceanDNA-b23388/target_genomes.txt --output OceanDNA-b23388/fastani_result.tsv --threads 1
[2023-03-19 03:50:32,394] [INFO] Task succeeded: fastANI
[2023-03-19 03:50:32,395] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3398f1b5-0ae0-44d0-9c75-4b4955f82b21/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 03:50:32,395] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3398f1b5-0ae0-44d0-9c75-4b4955f82b21/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 03:50:32,402] [INFO] Found 11 fastANI hits (0 hits with ANI > threshold)
[2023-03-19 03:50:32,402] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-19 03:50:32,402] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Brevundimonas basaltis	strain=DSM 25335	GCA_014202075.1	472166	472166	type	True	87.731	644	808	95	below_threshold
Brevundimonas alba	strain=DSM 4736	GCA_011927945.1	74314	74314	type	True	86.1357	660	808	95	below_threshold
Brevundimonas viscosa	strain=CGMCC 1.10683	GCA_900116065.1	871741	871741	type	True	84.1937	638	808	95	below_threshold
Brevundimonas lenta	strain=DSM 23960	GCA_014196335.1	424796	424796	type	True	84.1868	615	808	95	below_threshold
Brevundimonas albigilva	strain=KEME 9005-016	GCA_023503965.1	1312364	1312364	type	True	81.6325	528	808	95	below_threshold
Brevundimonas bullata	strain=HAMBI_262	GCA_003350205.1	13160	13160	type	True	81.3626	502	808	95	below_threshold
Brevundimonas fluminis	strain=LA-55	GCA_003934285.1	2487274	2487274	type	True	81.1347	507	808	95	below_threshold
Brevundimonas guildfordensis	strain=Sa3CVA3	GCA_014836405.1	2762241	2762241	type	True	80.9044	494	808	95	below_threshold
Brevundimonas vitisensis	strain=GR-TSA-9	GCA_016656965.1	2800818	2800818	type	True	80.0393	472	808	95	below_threshold
Phenylobacterium aquaticum	strain=KACC 18306	GCA_022695515.1	1763816	1763816	type	True	77.9548	324	808	95	below_threshold
Brevundimonas pishanensis	strain=CHPC 1.3453	GCA_022750635.1	2896315	2896315	type	True	77.935	274	808	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-19 03:50:32,403] [INFO] DFAST Taxonomy check result was written to OceanDNA-b23388/tc_result.tsv
[2023-03-19 03:50:32,403] [INFO] ===== Taxonomy check completed =====
[2023-03-19 03:50:32,403] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 03:50:32,403] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3398f1b5-0ae0-44d0-9c75-4b4955f82b21/dqc_reference/checkm_data
[2023-03-19 03:50:32,404] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 03:50:32,408] [INFO] Task started: CheckM
[2023-03-19 03:50:32,408] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b23388/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b23388/checkm_input OceanDNA-b23388/checkm_result
[2023-03-19 03:51:09,772] [INFO] Task succeeded: CheckM
[2023-03-19 03:51:09,773] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 50.69%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-19 03:51:09,775] [INFO] ===== Completeness check finished =====
[2023-03-19 03:51:09,775] [INFO] ===== Start GTDB Search =====
[2023-03-19 03:51:09,775] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b23388/markers.fasta)
[2023-03-19 03:51:09,776] [INFO] Task started: Blastn
[2023-03-19 03:51:09,776] [INFO] Running command: blastn -query OceanDNA-b23388/markers.fasta -db /var/lib/cwl/stg3398f1b5-0ae0-44d0-9c75-4b4955f82b21/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b23388/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 03:51:10,913] [INFO] Task succeeded: Blastn
[2023-03-19 03:51:10,914] [INFO] Selected 13 target genomes.
[2023-03-19 03:51:10,914] [INFO] Target genome list was writen to OceanDNA-b23388/target_genomes_gtdb.txt
[2023-03-19 03:51:10,932] [INFO] Task started: fastANI
[2023-03-19 03:51:10,932] [INFO] Running command: fastANI --query /var/lib/cwl/stg517f2889-1f7b-42fe-a3c1-a292136d415b/OceanDNA-b23388.fa --refList OceanDNA-b23388/target_genomes_gtdb.txt --output OceanDNA-b23388/fastani_result_gtdb.tsv --threads 1
[2023-03-19 03:51:19,648] [INFO] Task succeeded: fastANI
[2023-03-19 03:51:19,656] [INFO] Found 13 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-19 03:51:19,657] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018820665.1	s__Brevundimonas sp018820665	87.7477	561	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	99.88	99.88	0.93	0.93	2	-
GCF_014202075.1	s__Brevundimonas basaltis	87.7315	644	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016183935.1	s__Brevundimonas sp016183935	87.5828	613	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015234495.1	s__Brevundimonas sp015234495	86.5898	411	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_011927945.1	s__Brevundimonas alba	86.104	662	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_004296955.1	s__Brevundimonas sp004296955	85.5181	638	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	95.84	95.71	0.82	0.76	6	-
GCA_002483305.1	s__Brevundimonas sp002483305	85.2593	608	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018826515.1	s__Brevundimonas sp018826515	85.2394	616	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	99.85	99.80	0.97	0.95	6	-
GCF_001427825.1	s__Brevundimonas sp001427825	85.2111	628	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013912005.1	s__Brevundimonas sp013912005	84.3565	610	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_001794825.1	s__Brevundimonas sp001794825	84.2865	625	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014196335.1	s__Brevundimonas lenta	84.1922	614	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900116065.1	s__Brevundimonas viscosa	84.1874	639	808	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Brevundimonas	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-19 03:51:19,657] [INFO] GTDB search result was written to OceanDNA-b23388/result_gtdb.tsv
[2023-03-19 03:51:19,657] [INFO] ===== GTDB Search completed =====
[2023-03-19 03:51:19,658] [INFO] DFAST_QC result json was written to OceanDNA-b23388/dqc_result.json
[2023-03-19 03:51:19,658] [INFO] DFAST_QC completed!
[2023-03-19 03:51:19,659] [INFO] Total running time: 0h1m12s