[2023-03-16 23:23:12,000] [INFO] DFAST_QC pipeline started.
[2023-03-16 23:23:12,000] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 23:23:12,000] [INFO] DQC Reference Directory: /var/lib/cwl/stg4ee0274b-7c68-49dc-afe5-183efe95287d/dqc_reference
[2023-03-16 23:23:13,113] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 23:23:13,113] [INFO] Task started: Prodigal
[2023-03-16 23:23:13,114] [INFO] Running command: cat /var/lib/cwl/stg621d9209-56b7-4b85-a1e3-a144a7b3e8f4/OceanDNA-b24640.fa | prodigal -d OceanDNA-b24640/cds.fna -a OceanDNA-b24640/protein.faa -g 11 -q > /dev/null
[2023-03-16 23:23:28,357] [INFO] Task succeeded: Prodigal
[2023-03-16 23:23:28,358] [INFO] Task started: HMMsearch
[2023-03-16 23:23:28,358] [INFO] Running command: hmmsearch --tblout OceanDNA-b24640/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg4ee0274b-7c68-49dc-afe5-183efe95287d/dqc_reference/reference_markers.hmm OceanDNA-b24640/protein.faa > /dev/null
[2023-03-16 23:23:28,550] [INFO] Task succeeded: HMMsearch
[2023-03-16 23:23:28,551] [INFO] Found 6/6 markers.
[2023-03-16 23:23:28,568] [INFO] Query marker FASTA was written to OceanDNA-b24640/markers.fasta
[2023-03-16 23:23:28,568] [INFO] Task started: Blastn
[2023-03-16 23:23:28,568] [INFO] Running command: blastn -query OceanDNA-b24640/markers.fasta -db /var/lib/cwl/stg4ee0274b-7c68-49dc-afe5-183efe95287d/dqc_reference/reference_markers.fasta -out OceanDNA-b24640/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:23:29,142] [INFO] Task succeeded: Blastn
[2023-03-16 23:23:29,143] [INFO] Selected 28 target genomes.
[2023-03-16 23:23:29,143] [INFO] Target genome list was writen to OceanDNA-b24640/target_genomes.txt
[2023-03-16 23:23:29,158] [INFO] Task started: fastANI
[2023-03-16 23:23:29,159] [INFO] Running command: fastANI --query /var/lib/cwl/stg621d9209-56b7-4b85-a1e3-a144a7b3e8f4/OceanDNA-b24640.fa --refList OceanDNA-b24640/target_genomes.txt --output OceanDNA-b24640/fastani_result.tsv --threads 1
[2023-03-16 23:23:44,143] [INFO] Task succeeded: fastANI
[2023-03-16 23:23:44,143] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg4ee0274b-7c68-49dc-afe5-183efe95287d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 23:23:44,144] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg4ee0274b-7c68-49dc-afe5-183efe95287d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 23:23:44,144] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 23:23:44,144] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 23:23:44,144] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 23:23:44,144] [INFO] DFAST Taxonomy check result was written to OceanDNA-b24640/tc_result.tsv
[2023-03-16 23:23:44,144] [INFO] ===== Taxonomy check completed =====
[2023-03-16 23:23:44,144] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 23:23:44,144] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg4ee0274b-7c68-49dc-afe5-183efe95287d/dqc_reference/checkm_data
[2023-03-16 23:23:44,147] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 23:23:44,151] [INFO] Task started: CheckM
[2023-03-16 23:23:44,151] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b24640/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b24640/checkm_input OceanDNA-b24640/checkm_result
[2023-03-16 23:24:24,714] [INFO] Task succeeded: CheckM
[2023-03-16 23:24:24,714] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 79.17%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 23:24:24,716] [INFO] ===== Completeness check finished =====
[2023-03-16 23:24:24,717] [INFO] ===== Start GTDB Search =====
[2023-03-16 23:24:24,717] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b24640/markers.fasta)
[2023-03-16 23:24:24,718] [INFO] Task started: Blastn
[2023-03-16 23:24:24,718] [INFO] Running command: blastn -query OceanDNA-b24640/markers.fasta -db /var/lib/cwl/stg4ee0274b-7c68-49dc-afe5-183efe95287d/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b24640/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:24:25,693] [INFO] Task succeeded: Blastn
[2023-03-16 23:24:25,694] [INFO] Selected 26 target genomes.
[2023-03-16 23:24:25,694] [INFO] Target genome list was writen to OceanDNA-b24640/target_genomes_gtdb.txt
[2023-03-16 23:24:26,639] [INFO] Task started: fastANI
[2023-03-16 23:24:26,639] [INFO] Running command: fastANI --query /var/lib/cwl/stg621d9209-56b7-4b85-a1e3-a144a7b3e8f4/OceanDNA-b24640.fa --refList OceanDNA-b24640/target_genomes_gtdb.txt --output OceanDNA-b24640/fastani_result_gtdb.tsv --threads 1
[2023-03-16 23:24:37,466] [INFO] Task succeeded: fastANI
[2023-03-16 23:24:37,469] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-16 23:24:37,469] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002320665.1	s__UBA1672 sp002320665	99.8932	800	872	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Micavibrionales;f__Micavibrionaceae;g__UBA1672	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002401695.1	s__UBA1672 sp002401695	75.504	58	872	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Micavibrionales;f__Micavibrionaceae;g__UBA1672	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 23:24:37,469] [INFO] GTDB search result was written to OceanDNA-b24640/result_gtdb.tsv
[2023-03-16 23:24:37,469] [INFO] ===== GTDB Search completed =====
[2023-03-16 23:24:37,470] [INFO] DFAST_QC result json was written to OceanDNA-b24640/dqc_result.json
[2023-03-16 23:24:37,470] [INFO] DFAST_QC completed!
[2023-03-16 23:24:37,470] [INFO] Total running time: 0h1m25s