[2023-03-15 21:24:36,535] [INFO] DFAST_QC pipeline started.
[2023-03-15 21:24:36,535] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 21:24:36,535] [INFO] DQC Reference Directory: /var/lib/cwl/stge2c9c515-1098-412a-a6e8-29383e180341/dqc_reference
[2023-03-15 21:24:37,656] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 21:24:37,657] [INFO] Task started: Prodigal
[2023-03-15 21:24:37,657] [INFO] Running command: cat /var/lib/cwl/stge507a6b9-58e6-4d7f-bb6b-9eb51be4ae22/OceanDNA-b26197.fa | prodigal -d OceanDNA-b26197/cds.fna -a OceanDNA-b26197/protein.faa -g 11 -q > /dev/null
[2023-03-15 21:24:46,386] [INFO] Task succeeded: Prodigal
[2023-03-15 21:24:46,386] [INFO] Task started: HMMsearch
[2023-03-15 21:24:46,386] [INFO] Running command: hmmsearch --tblout OceanDNA-b26197/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge2c9c515-1098-412a-a6e8-29383e180341/dqc_reference/reference_markers.hmm OceanDNA-b26197/protein.faa > /dev/null
[2023-03-15 21:24:46,545] [INFO] Task succeeded: HMMsearch
[2023-03-15 21:24:46,545] [WARNING] Found 5/6 markers. [/var/lib/cwl/stge507a6b9-58e6-4d7f-bb6b-9eb51be4ae22/OceanDNA-b26197.fa]
[2023-03-15 21:24:46,557] [INFO] Query marker FASTA was written to OceanDNA-b26197/markers.fasta
[2023-03-15 21:24:46,557] [INFO] Task started: Blastn
[2023-03-15 21:24:46,558] [INFO] Running command: blastn -query OceanDNA-b26197/markers.fasta -db /var/lib/cwl/stge2c9c515-1098-412a-a6e8-29383e180341/dqc_reference/reference_markers.fasta -out OceanDNA-b26197/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 21:24:47,115] [INFO] Task succeeded: Blastn
[2023-03-15 21:24:47,116] [INFO] Selected 30 target genomes.
[2023-03-15 21:24:47,116] [INFO] Target genome list was writen to OceanDNA-b26197/target_genomes.txt
[2023-03-15 21:24:47,133] [INFO] Task started: fastANI
[2023-03-15 21:24:47,133] [INFO] Running command: fastANI --query /var/lib/cwl/stge507a6b9-58e6-4d7f-bb6b-9eb51be4ae22/OceanDNA-b26197.fa --refList OceanDNA-b26197/target_genomes.txt --output OceanDNA-b26197/fastani_result.tsv --threads 1
[2023-03-15 21:25:02,756] [INFO] Task succeeded: fastANI
[2023-03-15 21:25:02,757] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stge2c9c515-1098-412a-a6e8-29383e180341/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 21:25:02,757] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stge2c9c515-1098-412a-a6e8-29383e180341/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 21:25:02,757] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 21:25:02,757] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 21:25:02,757] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 21:25:02,757] [INFO] DFAST Taxonomy check result was written to OceanDNA-b26197/tc_result.tsv
[2023-03-15 21:25:02,757] [INFO] ===== Taxonomy check completed =====
[2023-03-15 21:25:02,757] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 21:25:02,758] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge2c9c515-1098-412a-a6e8-29383e180341/dqc_reference/checkm_data
[2023-03-15 21:25:02,760] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 21:25:02,763] [INFO] Task started: CheckM
[2023-03-15 21:25:02,763] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b26197/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b26197/checkm_input OceanDNA-b26197/checkm_result
[2023-03-15 21:25:29,883] [INFO] Task succeeded: CheckM
[2023-03-15 21:25:29,883] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 86.92%
Contamintation: 0.46%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-15 21:25:29,885] [INFO] ===== Completeness check finished =====
[2023-03-15 21:25:29,885] [INFO] ===== Start GTDB Search =====
[2023-03-15 21:25:29,885] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b26197/markers.fasta)
[2023-03-15 21:25:29,885] [INFO] Task started: Blastn
[2023-03-15 21:25:29,885] [INFO] Running command: blastn -query OceanDNA-b26197/markers.fasta -db /var/lib/cwl/stge2c9c515-1098-412a-a6e8-29383e180341/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b26197/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 21:25:30,761] [INFO] Task succeeded: Blastn
[2023-03-15 21:25:30,762] [INFO] Selected 15 target genomes.
[2023-03-15 21:25:30,762] [INFO] Target genome list was writen to OceanDNA-b26197/target_genomes_gtdb.txt
[2023-03-15 21:25:30,952] [INFO] Task started: fastANI
[2023-03-15 21:25:30,953] [INFO] Running command: fastANI --query /var/lib/cwl/stge507a6b9-58e6-4d7f-bb6b-9eb51be4ae22/OceanDNA-b26197.fa --refList OceanDNA-b26197/target_genomes_gtdb.txt --output OceanDNA-b26197/fastani_result_gtdb.tsv --threads 1
[2023-03-15 21:25:36,150] [INFO] Task succeeded: fastANI
[2023-03-15 21:25:36,155] [INFO] Found 8 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 21:25:36,155] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_902583715.1	s__UBA3439 sp902583715	94.4397	234	446	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Puniceispirillales;f__Puniceispirillaceae;g__UBA3439	95.0	95.85	95.85	0.55	0.55	2	-
GCA_016780525.1	s__UBA3439 sp016780525	79.6095	216	446	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Puniceispirillales;f__Puniceispirillaceae;g__UBA3439	95.0	99.22	99.22	0.71	0.71	2	-
GCA_905182545.1	s__UBA3439 sp905182545	77.2281	134	446	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Puniceispirillales;f__Puniceispirillaceae;g__UBA3439	95.0	N/A	N/A	N/A	N/A	1	-
GCA_009919335.1	s__UBA3439 sp009919335	76.9043	100	446	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Puniceispirillales;f__Puniceispirillaceae;g__UBA3439	95.0	98.81	98.81	0.76	0.76	2	-
GCA_017859295.1	s__UBA3439 sp017859295	76.8842	123	446	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Puniceispirillales;f__Puniceispirillaceae;g__UBA3439	95.0	98.52	98.52	0.78	0.78	2	-
GCA_002378605.1	s__UBA3439 sp002378605	76.702	119	446	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Puniceispirillales;f__Puniceispirillaceae;g__UBA3439	95.0	99.28	99.28	0.80	0.80	2	-
GCA_016778895.1	s__UBA3439 sp016778895	76.661	104	446	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Puniceispirillales;f__Puniceispirillaceae;g__UBA3439	95.0	99.63	99.63	0.87	0.87	2	-
GCA_004212735.1	s__MED-G116 sp004212735	76.3713	54	446	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Puniceispirillales;f__Puniceispirillaceae;g__MED-G116	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 21:25:36,156] [INFO] GTDB search result was written to OceanDNA-b26197/result_gtdb.tsv
[2023-03-15 21:25:36,156] [INFO] ===== GTDB Search completed =====
[2023-03-15 21:25:36,156] [INFO] DFAST_QC result json was written to OceanDNA-b26197/dqc_result.json
[2023-03-15 21:25:36,157] [INFO] DFAST_QC completed!
[2023-03-15 21:25:36,157] [INFO] Total running time: 0h0m60s