[2023-03-16 10:57:32,762] [INFO] DFAST_QC pipeline started.
[2023-03-16 10:57:32,762] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 10:57:32,762] [INFO] DQC Reference Directory: /var/lib/cwl/stg6d8e2a2b-1dda-43a4-b8b7-fa70ec647e40/dqc_reference
[2023-03-16 10:57:33,887] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 10:57:33,888] [INFO] Task started: Prodigal
[2023-03-16 10:57:33,888] [INFO] Running command: cat /var/lib/cwl/stgf9ce5f14-f424-4c76-962d-e8f5149c93a4/OceanDNA-b26260.fa | prodigal -d OceanDNA-b26260/cds.fna -a OceanDNA-b26260/protein.faa -g 11 -q > /dev/null
[2023-03-16 10:57:39,750] [INFO] Task succeeded: Prodigal
[2023-03-16 10:57:39,750] [INFO] Task started: HMMsearch
[2023-03-16 10:57:39,750] [INFO] Running command: hmmsearch --tblout OceanDNA-b26260/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6d8e2a2b-1dda-43a4-b8b7-fa70ec647e40/dqc_reference/reference_markers.hmm OceanDNA-b26260/protein.faa > /dev/null
[2023-03-16 10:57:39,907] [INFO] Task succeeded: HMMsearch
[2023-03-16 10:57:39,907] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgf9ce5f14-f424-4c76-962d-e8f5149c93a4/OceanDNA-b26260.fa]
[2023-03-16 10:57:39,920] [INFO] Query marker FASTA was written to OceanDNA-b26260/markers.fasta
[2023-03-16 10:57:39,920] [INFO] Task started: Blastn
[2023-03-16 10:57:39,921] [INFO] Running command: blastn -query OceanDNA-b26260/markers.fasta -db /var/lib/cwl/stg6d8e2a2b-1dda-43a4-b8b7-fa70ec647e40/dqc_reference/reference_markers.fasta -out OceanDNA-b26260/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 10:57:40,441] [INFO] Task succeeded: Blastn
[2023-03-16 10:57:40,442] [INFO] Selected 29 target genomes.
[2023-03-16 10:57:40,442] [INFO] Target genome list was writen to OceanDNA-b26260/target_genomes.txt
[2023-03-16 10:57:40,458] [INFO] Task started: fastANI
[2023-03-16 10:57:40,458] [INFO] Running command: fastANI --query /var/lib/cwl/stgf9ce5f14-f424-4c76-962d-e8f5149c93a4/OceanDNA-b26260.fa --refList OceanDNA-b26260/target_genomes.txt --output OceanDNA-b26260/fastani_result.tsv --threads 1
[2023-03-16 10:57:54,217] [INFO] Task succeeded: fastANI
[2023-03-16 10:57:54,217] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6d8e2a2b-1dda-43a4-b8b7-fa70ec647e40/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 10:57:54,217] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6d8e2a2b-1dda-43a4-b8b7-fa70ec647e40/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 10:57:54,218] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 10:57:54,218] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 10:57:54,218] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 10:57:54,218] [INFO] DFAST Taxonomy check result was written to OceanDNA-b26260/tc_result.tsv
[2023-03-16 10:57:54,218] [INFO] ===== Taxonomy check completed =====
[2023-03-16 10:57:54,218] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 10:57:54,218] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6d8e2a2b-1dda-43a4-b8b7-fa70ec647e40/dqc_reference/checkm_data
[2023-03-16 10:57:54,221] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 10:57:54,224] [INFO] Task started: CheckM
[2023-03-16 10:57:54,224] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b26260/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b26260/checkm_input OceanDNA-b26260/checkm_result
[2023-03-16 10:58:15,237] [INFO] Task succeeded: CheckM
[2023-03-16 10:58:15,237] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 89.58%
Contamintation: 1.85%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 10:58:15,239] [INFO] ===== Completeness check finished =====
[2023-03-16 10:58:15,239] [INFO] ===== Start GTDB Search =====
[2023-03-16 10:58:15,239] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b26260/markers.fasta)
[2023-03-16 10:58:15,240] [INFO] Task started: Blastn
[2023-03-16 10:58:15,240] [INFO] Running command: blastn -query OceanDNA-b26260/markers.fasta -db /var/lib/cwl/stg6d8e2a2b-1dda-43a4-b8b7-fa70ec647e40/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b26260/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 10:58:16,014] [INFO] Task succeeded: Blastn
[2023-03-16 10:58:16,015] [INFO] Selected 29 target genomes.
[2023-03-16 10:58:16,015] [INFO] Target genome list was writen to OceanDNA-b26260/target_genomes_gtdb.txt
[2023-03-16 10:58:16,091] [INFO] Task started: fastANI
[2023-03-16 10:58:16,091] [INFO] Running command: fastANI --query /var/lib/cwl/stgf9ce5f14-f424-4c76-962d-e8f5149c93a4/OceanDNA-b26260.fa --refList OceanDNA-b26260/target_genomes_gtdb.txt --output OceanDNA-b26260/fastani_result_gtdb.tsv --threads 1
[2023-03-16 10:58:30,015] [INFO] Task succeeded: fastANI
[2023-03-16 10:58:30,017] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 10:58:30,017] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002387605.1	s__UBA4588 sp002387605	86.0657	247	267	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Puniceispirillales;f__Puniceispirillaceae;g__UBA4588	95.0	99.40	98.72	0.91	0.85	18	-
--------------------------------------------------------------------------------
[2023-03-16 10:58:30,017] [INFO] GTDB search result was written to OceanDNA-b26260/result_gtdb.tsv
[2023-03-16 10:58:30,017] [INFO] ===== GTDB Search completed =====
[2023-03-16 10:58:30,018] [INFO] DFAST_QC result json was written to OceanDNA-b26260/dqc_result.json
[2023-03-16 10:58:30,018] [INFO] DFAST_QC completed!
[2023-03-16 10:58:30,018] [INFO] Total running time: 0h0m57s