[2023-03-15 11:51:17,347] [INFO] DFAST_QC pipeline started.
[2023-03-15 11:51:17,347] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 11:51:17,347] [INFO] DQC Reference Directory: /var/lib/cwl/stg90ca78f9-3838-4073-aaa7-40848929ae0a/dqc_reference
[2023-03-15 11:51:18,950] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 11:51:18,950] [INFO] Task started: Prodigal
[2023-03-15 11:51:18,950] [INFO] Running command: cat /var/lib/cwl/stg3696468a-60cc-442c-8947-e9ff76119050/OceanDNA-b282.fa | prodigal -d OceanDNA-b282/cds.fna -a OceanDNA-b282/protein.faa -g 11 -q > /dev/null
[2023-03-15 11:51:24,583] [INFO] Task succeeded: Prodigal
[2023-03-15 11:51:24,583] [INFO] Task started: HMMsearch
[2023-03-15 11:51:24,583] [INFO] Running command: hmmsearch --tblout OceanDNA-b282/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg90ca78f9-3838-4073-aaa7-40848929ae0a/dqc_reference/reference_markers.hmm OceanDNA-b282/protein.faa > /dev/null
[2023-03-15 11:51:24,740] [INFO] Task succeeded: HMMsearch
[2023-03-15 11:51:24,741] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg3696468a-60cc-442c-8947-e9ff76119050/OceanDNA-b282.fa]
[2023-03-15 11:51:24,754] [INFO] Query marker FASTA was written to OceanDNA-b282/markers.fasta
[2023-03-15 11:51:24,754] [INFO] Task started: Blastn
[2023-03-15 11:51:24,755] [INFO] Running command: blastn -query OceanDNA-b282/markers.fasta -db /var/lib/cwl/stg90ca78f9-3838-4073-aaa7-40848929ae0a/dqc_reference/reference_markers.fasta -out OceanDNA-b282/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 11:51:26,011] [INFO] Task succeeded: Blastn
[2023-03-15 11:51:26,012] [INFO] Selected 25 target genomes.
[2023-03-15 11:51:26,012] [INFO] Target genome list was writen to OceanDNA-b282/target_genomes.txt
[2023-03-15 11:51:26,029] [INFO] Task started: fastANI
[2023-03-15 11:51:26,030] [INFO] Running command: fastANI --query /var/lib/cwl/stg3696468a-60cc-442c-8947-e9ff76119050/OceanDNA-b282.fa --refList OceanDNA-b282/target_genomes.txt --output OceanDNA-b282/fastani_result.tsv --threads 1
[2023-03-15 11:51:42,851] [INFO] Task succeeded: fastANI
[2023-03-15 11:51:42,852] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg90ca78f9-3838-4073-aaa7-40848929ae0a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 11:51:42,852] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg90ca78f9-3838-4073-aaa7-40848929ae0a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 11:51:42,852] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 11:51:42,852] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 11:51:42,852] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 11:51:42,852] [INFO] DFAST Taxonomy check result was written to OceanDNA-b282/tc_result.tsv
[2023-03-15 11:51:42,852] [INFO] ===== Taxonomy check completed =====
[2023-03-15 11:51:42,852] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 11:51:42,853] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg90ca78f9-3838-4073-aaa7-40848929ae0a/dqc_reference/checkm_data
[2023-03-15 11:51:42,856] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 11:51:42,859] [INFO] Task started: CheckM
[2023-03-15 11:51:42,859] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b282/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b282/checkm_input OceanDNA-b282/checkm_result
[2023-03-15 11:52:03,473] [INFO] Task succeeded: CheckM
[2023-03-15 11:52:03,473] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 58.84%
Contamintation: 0.52%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 11:52:03,475] [INFO] ===== Completeness check finished =====
[2023-03-15 11:52:03,475] [INFO] ===== Start GTDB Search =====
[2023-03-15 11:52:03,475] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b282/markers.fasta)
[2023-03-15 11:52:03,476] [INFO] Task started: Blastn
[2023-03-15 11:52:03,476] [INFO] Running command: blastn -query OceanDNA-b282/markers.fasta -db /var/lib/cwl/stg90ca78f9-3838-4073-aaa7-40848929ae0a/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b282/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 11:52:04,323] [INFO] Task succeeded: Blastn
[2023-03-15 11:52:04,324] [INFO] Selected 19 target genomes.
[2023-03-15 11:52:04,324] [INFO] Target genome list was writen to OceanDNA-b282/target_genomes_gtdb.txt
[2023-03-15 11:52:04,407] [INFO] Task started: fastANI
[2023-03-15 11:52:04,407] [INFO] Running command: fastANI --query /var/lib/cwl/stg3696468a-60cc-442c-8947-e9ff76119050/OceanDNA-b282.fa --refList OceanDNA-b282/target_genomes_gtdb.txt --output OceanDNA-b282/fastani_result_gtdb.tsv --threads 1
[2023-03-15 11:52:16,556] [INFO] Task succeeded: fastANI
[2023-03-15 11:52:16,559] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 11:52:16,559] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002712885.1	s__UBA8438 sp002712885	79.6409	97	135	d__Bacteria;p__Acidobacteriota;c__Vicinamibacteria;o__Vicinamibacterales;f__UBA8438;g__UBA8438	95.0	N/A	N/A	N/A	N/A	1	-
GCA_005778315.1	s__UBA8438 sp005778315	78.1442	65	135	d__Bacteria;p__Acidobacteriota;c__Vicinamibacteria;o__Vicinamibacterales;f__UBA8438;g__UBA8438	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 11:52:16,559] [INFO] GTDB search result was written to OceanDNA-b282/result_gtdb.tsv
[2023-03-15 11:52:16,559] [INFO] ===== GTDB Search completed =====
[2023-03-15 11:52:16,560] [INFO] DFAST_QC result json was written to OceanDNA-b282/dqc_result.json
[2023-03-15 11:52:16,560] [INFO] DFAST_QC completed!
[2023-03-15 11:52:16,560] [INFO] Total running time: 0h0m59s