[2023-03-15 03:02:04,957] [INFO] DFAST_QC pipeline started.
[2023-03-15 03:02:04,957] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 03:02:04,957] [INFO] DQC Reference Directory: /var/lib/cwl/stg18ab4d48-22f2-4f77-900c-4675d6eb9ff9/dqc_reference
[2023-03-15 03:02:06,576] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 03:02:06,576] [INFO] Task started: Prodigal
[2023-03-15 03:02:06,577] [INFO] Running command: cat /var/lib/cwl/stg6372e5cc-c6cf-4db8-b340-a91dc46f33f8/OceanDNA-b29728.fa | prodigal -d OceanDNA-b29728/cds.fna -a OceanDNA-b29728/protein.faa -g 11 -q > /dev/null
[2023-03-15 03:02:13,986] [INFO] Task succeeded: Prodigal
[2023-03-15 03:02:13,987] [INFO] Task started: HMMsearch
[2023-03-15 03:02:13,987] [INFO] Running command: hmmsearch --tblout OceanDNA-b29728/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg18ab4d48-22f2-4f77-900c-4675d6eb9ff9/dqc_reference/reference_markers.hmm OceanDNA-b29728/protein.faa > /dev/null
[2023-03-15 03:02:14,135] [INFO] Task succeeded: HMMsearch
[2023-03-15 03:02:14,135] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg6372e5cc-c6cf-4db8-b340-a91dc46f33f8/OceanDNA-b29728.fa]
[2023-03-15 03:02:14,149] [INFO] Query marker FASTA was written to OceanDNA-b29728/markers.fasta
[2023-03-15 03:02:14,151] [INFO] Task started: Blastn
[2023-03-15 03:02:14,151] [INFO] Running command: blastn -query OceanDNA-b29728/markers.fasta -db /var/lib/cwl/stg18ab4d48-22f2-4f77-900c-4675d6eb9ff9/dqc_reference/reference_markers.fasta -out OceanDNA-b29728/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 03:02:14,654] [INFO] Task succeeded: Blastn
[2023-03-15 03:02:14,655] [INFO] Selected 30 target genomes.
[2023-03-15 03:02:14,655] [INFO] Target genome list was writen to OceanDNA-b29728/target_genomes.txt
[2023-03-15 03:02:14,673] [INFO] Task started: fastANI
[2023-03-15 03:02:14,673] [INFO] Running command: fastANI --query /var/lib/cwl/stg6372e5cc-c6cf-4db8-b340-a91dc46f33f8/OceanDNA-b29728.fa --refList OceanDNA-b29728/target_genomes.txt --output OceanDNA-b29728/fastani_result.tsv --threads 1
[2023-03-15 03:02:30,768] [INFO] Task succeeded: fastANI
[2023-03-15 03:02:30,769] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg18ab4d48-22f2-4f77-900c-4675d6eb9ff9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 03:02:30,769] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg18ab4d48-22f2-4f77-900c-4675d6eb9ff9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 03:02:30,769] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 03:02:30,769] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 03:02:30,769] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 03:02:30,769] [INFO] DFAST Taxonomy check result was written to OceanDNA-b29728/tc_result.tsv
[2023-03-15 03:02:30,770] [INFO] ===== Taxonomy check completed =====
[2023-03-15 03:02:30,770] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 03:02:30,770] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg18ab4d48-22f2-4f77-900c-4675d6eb9ff9/dqc_reference/checkm_data
[2023-03-15 03:02:30,772] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 03:02:30,781] [INFO] Task started: CheckM
[2023-03-15 03:02:30,781] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b29728/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b29728/checkm_input OceanDNA-b29728/checkm_result
[2023-03-15 03:02:55,319] [INFO] Task succeeded: CheckM
[2023-03-15 03:02:55,319] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 89.02%
Contamintation: 8.33%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-03-15 03:02:55,322] [INFO] ===== Completeness check finished =====
[2023-03-15 03:02:55,322] [INFO] ===== Start GTDB Search =====
[2023-03-15 03:02:55,322] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b29728/markers.fasta)
[2023-03-15 03:02:55,323] [INFO] Task started: Blastn
[2023-03-15 03:02:55,323] [INFO] Running command: blastn -query OceanDNA-b29728/markers.fasta -db /var/lib/cwl/stg18ab4d48-22f2-4f77-900c-4675d6eb9ff9/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b29728/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 03:02:56,056] [INFO] Task succeeded: Blastn
[2023-03-15 03:02:56,057] [INFO] Selected 29 target genomes.
[2023-03-15 03:02:56,057] [INFO] Target genome list was writen to OceanDNA-b29728/target_genomes_gtdb.txt
[2023-03-15 03:02:56,079] [INFO] Task started: fastANI
[2023-03-15 03:02:56,080] [INFO] Running command: fastANI --query /var/lib/cwl/stg6372e5cc-c6cf-4db8-b340-a91dc46f33f8/OceanDNA-b29728.fa --refList OceanDNA-b29728/target_genomes_gtdb.txt --output OceanDNA-b29728/fastani_result_gtdb.tsv --threads 1
[2023-03-15 03:03:11,384] [INFO] Task succeeded: fastANI
[2023-03-15 03:03:11,385] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 03:03:11,385] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-15 03:03:11,385] [INFO] GTDB search result was written to OceanDNA-b29728/result_gtdb.tsv
[2023-03-15 03:03:11,385] [INFO] ===== GTDB Search completed =====
[2023-03-15 03:03:11,386] [INFO] DFAST_QC result json was written to OceanDNA-b29728/dqc_result.json
[2023-03-15 03:03:11,386] [INFO] DFAST_QC completed!
[2023-03-15 03:03:11,386] [INFO] Total running time: 0h1m6s