[2023-03-19 00:33:19,080] [INFO] DFAST_QC pipeline started.
[2023-03-19 00:33:19,081] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 00:33:19,081] [INFO] DQC Reference Directory: /var/lib/cwl/stg769b2ad6-fdfc-4d96-8b90-381788b76e1f/dqc_reference
[2023-03-19 00:33:20,221] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 00:33:20,222] [INFO] Task started: Prodigal
[2023-03-19 00:33:20,222] [INFO] Running command: cat /var/lib/cwl/stg1c08c494-a970-4d0c-8022-e8b2e64733a2/OceanDNA-b31335.fa | prodigal -d OceanDNA-b31335/cds.fna -a OceanDNA-b31335/protein.faa -g 11 -q > /dev/null
[2023-03-19 00:33:26,543] [INFO] Task succeeded: Prodigal
[2023-03-19 00:33:26,543] [INFO] Task started: HMMsearch
[2023-03-19 00:33:26,543] [INFO] Running command: hmmsearch --tblout OceanDNA-b31335/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg769b2ad6-fdfc-4d96-8b90-381788b76e1f/dqc_reference/reference_markers.hmm OceanDNA-b31335/protein.faa > /dev/null
[2023-03-19 00:33:26,744] [INFO] Task succeeded: HMMsearch
[2023-03-19 00:33:26,744] [INFO] Found 6/6 markers.
[2023-03-19 00:33:26,759] [INFO] Query marker FASTA was written to OceanDNA-b31335/markers.fasta
[2023-03-19 00:33:26,759] [INFO] Task started: Blastn
[2023-03-19 00:33:26,760] [INFO] Running command: blastn -query OceanDNA-b31335/markers.fasta -db /var/lib/cwl/stg769b2ad6-fdfc-4d96-8b90-381788b76e1f/dqc_reference/reference_markers.fasta -out OceanDNA-b31335/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 00:33:27,307] [INFO] Task succeeded: Blastn
[2023-03-19 00:33:27,309] [INFO] Selected 25 target genomes.
[2023-03-19 00:33:27,309] [INFO] Target genome list was writen to OceanDNA-b31335/target_genomes.txt
[2023-03-19 00:33:27,332] [INFO] Task started: fastANI
[2023-03-19 00:33:27,332] [INFO] Running command: fastANI --query /var/lib/cwl/stg1c08c494-a970-4d0c-8022-e8b2e64733a2/OceanDNA-b31335.fa --refList OceanDNA-b31335/target_genomes.txt --output OceanDNA-b31335/fastani_result.tsv --threads 1
[2023-03-19 00:33:35,322] [INFO] Task succeeded: fastANI
[2023-03-19 00:33:35,322] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg769b2ad6-fdfc-4d96-8b90-381788b76e1f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 00:33:35,322] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg769b2ad6-fdfc-4d96-8b90-381788b76e1f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 00:33:35,322] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-19 00:33:35,323] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-19 00:33:35,323] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-19 00:33:35,326] [INFO] DFAST Taxonomy check result was written to OceanDNA-b31335/tc_result.tsv
[2023-03-19 00:33:35,329] [INFO] ===== Taxonomy check completed =====
[2023-03-19 00:33:35,329] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 00:33:35,329] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg769b2ad6-fdfc-4d96-8b90-381788b76e1f/dqc_reference/checkm_data
[2023-03-19 00:33:35,332] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 00:33:35,335] [INFO] Task started: CheckM
[2023-03-19 00:33:35,336] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b31335/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b31335/checkm_input OceanDNA-b31335/checkm_result
[2023-03-19 00:33:57,070] [INFO] Task succeeded: CheckM
[2023-03-19 00:33:57,070] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-19 00:33:57,074] [INFO] ===== Completeness check finished =====
[2023-03-19 00:33:57,074] [INFO] ===== Start GTDB Search =====
[2023-03-19 00:33:57,075] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b31335/markers.fasta)
[2023-03-19 00:33:57,076] [INFO] Task started: Blastn
[2023-03-19 00:33:57,076] [INFO] Running command: blastn -query OceanDNA-b31335/markers.fasta -db /var/lib/cwl/stg769b2ad6-fdfc-4d96-8b90-381788b76e1f/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b31335/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 00:33:57,836] [INFO] Task succeeded: Blastn
[2023-03-19 00:33:57,838] [INFO] Selected 23 target genomes.
[2023-03-19 00:33:57,838] [INFO] Target genome list was writen to OceanDNA-b31335/target_genomes_gtdb.txt
[2023-03-19 00:33:57,902] [INFO] Task started: fastANI
[2023-03-19 00:33:57,903] [INFO] Running command: fastANI --query /var/lib/cwl/stg1c08c494-a970-4d0c-8022-e8b2e64733a2/OceanDNA-b31335.fa --refList OceanDNA-b31335/target_genomes_gtdb.txt --output OceanDNA-b31335/fastani_result_gtdb.tsv --threads 1
[2023-03-19 00:34:04,359] [INFO] Task succeeded: fastANI
[2023-03-19 00:34:04,361] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-19 00:34:04,361] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_905479885.1	s__GCA-2402195 sp905479885	76.3703	62	410	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rickettsiales;f__Rickettsiaceae;g__GCA-2402195	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-19 00:34:04,362] [INFO] GTDB search result was written to OceanDNA-b31335/result_gtdb.tsv
[2023-03-19 00:34:04,362] [INFO] ===== GTDB Search completed =====
[2023-03-19 00:34:04,364] [INFO] DFAST_QC result json was written to OceanDNA-b31335/dqc_result.json
[2023-03-19 00:34:04,364] [INFO] DFAST_QC completed!
[2023-03-19 00:34:04,365] [INFO] Total running time: 0h0m45s