[2023-03-16 23:05:03,929] [INFO] DFAST_QC pipeline started.
[2023-03-16 23:05:03,929] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 23:05:03,930] [INFO] DQC Reference Directory: /var/lib/cwl/stgbc7c0fcf-5c43-4b50-a28c-2065f38629f9/dqc_reference
[2023-03-16 23:05:05,191] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 23:05:05,191] [INFO] Task started: Prodigal
[2023-03-16 23:05:05,191] [INFO] Running command: cat /var/lib/cwl/stgd4ee1f01-a339-46db-b6e0-66a30fca2075/OceanDNA-b31470.fa | prodigal -d OceanDNA-b31470/cds.fna -a OceanDNA-b31470/protein.faa -g 11 -q > /dev/null
[2023-03-16 23:05:10,221] [INFO] Task succeeded: Prodigal
[2023-03-16 23:05:10,221] [INFO] Task started: HMMsearch
[2023-03-16 23:05:10,222] [INFO] Running command: hmmsearch --tblout OceanDNA-b31470/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgbc7c0fcf-5c43-4b50-a28c-2065f38629f9/dqc_reference/reference_markers.hmm OceanDNA-b31470/protein.faa > /dev/null
[2023-03-16 23:05:10,357] [INFO] Task succeeded: HMMsearch
[2023-03-16 23:05:10,357] [WARNING] Found 3/6 markers. [/var/lib/cwl/stgd4ee1f01-a339-46db-b6e0-66a30fca2075/OceanDNA-b31470.fa]
[2023-03-16 23:05:10,368] [INFO] Query marker FASTA was written to OceanDNA-b31470/markers.fasta
[2023-03-16 23:05:10,368] [INFO] Task started: Blastn
[2023-03-16 23:05:10,368] [INFO] Running command: blastn -query OceanDNA-b31470/markers.fasta -db /var/lib/cwl/stgbc7c0fcf-5c43-4b50-a28c-2065f38629f9/dqc_reference/reference_markers.fasta -out OceanDNA-b31470/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:05:10,903] [INFO] Task succeeded: Blastn
[2023-03-16 23:05:10,903] [INFO] Selected 15 target genomes.
[2023-03-16 23:05:10,904] [INFO] Target genome list was writen to OceanDNA-b31470/target_genomes.txt
[2023-03-16 23:05:10,912] [INFO] Task started: fastANI
[2023-03-16 23:05:10,912] [INFO] Running command: fastANI --query /var/lib/cwl/stgd4ee1f01-a339-46db-b6e0-66a30fca2075/OceanDNA-b31470.fa --refList OceanDNA-b31470/target_genomes.txt --output OceanDNA-b31470/fastani_result.tsv --threads 1
[2023-03-16 23:05:19,879] [INFO] Task succeeded: fastANI
[2023-03-16 23:05:19,879] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgbc7c0fcf-5c43-4b50-a28c-2065f38629f9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 23:05:19,879] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgbc7c0fcf-5c43-4b50-a28c-2065f38629f9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 23:05:19,879] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 23:05:19,880] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 23:05:19,880] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 23:05:19,880] [INFO] DFAST Taxonomy check result was written to OceanDNA-b31470/tc_result.tsv
[2023-03-16 23:05:19,880] [INFO] ===== Taxonomy check completed =====
[2023-03-16 23:05:19,880] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 23:05:19,880] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgbc7c0fcf-5c43-4b50-a28c-2065f38629f9/dqc_reference/checkm_data
[2023-03-16 23:05:19,883] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 23:05:19,885] [INFO] Task started: CheckM
[2023-03-16 23:05:19,885] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b31470/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b31470/checkm_input OceanDNA-b31470/checkm_result
[2023-03-16 23:05:38,608] [INFO] Task succeeded: CheckM
[2023-03-16 23:05:38,608] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 76.39%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 23:05:38,610] [INFO] ===== Completeness check finished =====
[2023-03-16 23:05:38,610] [INFO] ===== Start GTDB Search =====
[2023-03-16 23:05:38,611] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b31470/markers.fasta)
[2023-03-16 23:05:38,611] [INFO] Task started: Blastn
[2023-03-16 23:05:38,611] [INFO] Running command: blastn -query OceanDNA-b31470/markers.fasta -db /var/lib/cwl/stgbc7c0fcf-5c43-4b50-a28c-2065f38629f9/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b31470/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 23:05:39,382] [INFO] Task succeeded: Blastn
[2023-03-16 23:05:39,383] [INFO] Selected 14 target genomes.
[2023-03-16 23:05:39,383] [INFO] Target genome list was writen to OceanDNA-b31470/target_genomes_gtdb.txt
[2023-03-16 23:05:39,811] [INFO] Task started: fastANI
[2023-03-16 23:05:39,811] [INFO] Running command: fastANI --query /var/lib/cwl/stgd4ee1f01-a339-46db-b6e0-66a30fca2075/OceanDNA-b31470.fa --refList OceanDNA-b31470/target_genomes_gtdb.txt --output OceanDNA-b31470/fastani_result_gtdb.tsv --threads 1
[2023-03-16 23:05:47,141] [INFO] Task succeeded: fastANI
[2023-03-16 23:05:47,143] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 23:05:47,143] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_004356955.1	s__SMXS01 sp004356955	78.1911	78	177	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__SMXS01;f__SMXS01;g__SMXS01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 23:05:47,144] [INFO] GTDB search result was written to OceanDNA-b31470/result_gtdb.tsv
[2023-03-16 23:05:47,144] [INFO] ===== GTDB Search completed =====
[2023-03-16 23:05:47,144] [INFO] DFAST_QC result json was written to OceanDNA-b31470/dqc_result.json
[2023-03-16 23:05:47,144] [INFO] DFAST_QC completed!
[2023-03-16 23:05:47,144] [INFO] Total running time: 0h0m43s