[2023-03-18 08:31:56,276] [INFO] DFAST_QC pipeline started.
[2023-03-18 08:31:56,276] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 08:31:56,276] [INFO] DQC Reference Directory: /var/lib/cwl/stg0c9161ab-1250-472b-8f82-ce2842aaf6fe/dqc_reference
[2023-03-18 08:31:57,410] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 08:31:57,411] [INFO] Task started: Prodigal
[2023-03-18 08:31:57,411] [INFO] Running command: cat /var/lib/cwl/stg4ca8a219-ee26-4cb7-997e-db97d982adba/OceanDNA-b32470.fa | prodigal -d OceanDNA-b32470/cds.fna -a OceanDNA-b32470/protein.faa -g 11 -q > /dev/null
[2023-03-18 08:32:02,514] [INFO] Task succeeded: Prodigal
[2023-03-18 08:32:02,515] [INFO] Task started: HMMsearch
[2023-03-18 08:32:02,515] [INFO] Running command: hmmsearch --tblout OceanDNA-b32470/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg0c9161ab-1250-472b-8f82-ce2842aaf6fe/dqc_reference/reference_markers.hmm OceanDNA-b32470/protein.faa > /dev/null
[2023-03-18 08:32:02,669] [INFO] Task succeeded: HMMsearch
[2023-03-18 08:32:02,670] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg4ca8a219-ee26-4cb7-997e-db97d982adba/OceanDNA-b32470.fa]
[2023-03-18 08:32:02,678] [INFO] Query marker FASTA was written to OceanDNA-b32470/markers.fasta
[2023-03-18 08:32:02,678] [INFO] Task started: Blastn
[2023-03-18 08:32:02,678] [INFO] Running command: blastn -query OceanDNA-b32470/markers.fasta -db /var/lib/cwl/stg0c9161ab-1250-472b-8f82-ce2842aaf6fe/dqc_reference/reference_markers.fasta -out OceanDNA-b32470/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 08:32:03,104] [INFO] Task succeeded: Blastn
[2023-03-18 08:32:03,105] [INFO] Selected 7 target genomes.
[2023-03-18 08:32:03,105] [INFO] Target genome list was writen to OceanDNA-b32470/target_genomes.txt
[2023-03-18 08:32:03,109] [INFO] Task started: fastANI
[2023-03-18 08:32:03,109] [INFO] Running command: fastANI --query /var/lib/cwl/stg4ca8a219-ee26-4cb7-997e-db97d982adba/OceanDNA-b32470.fa --refList OceanDNA-b32470/target_genomes.txt --output OceanDNA-b32470/fastani_result.tsv --threads 1
[2023-03-18 08:32:07,152] [INFO] Task succeeded: fastANI
[2023-03-18 08:32:07,152] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg0c9161ab-1250-472b-8f82-ce2842aaf6fe/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 08:32:07,152] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg0c9161ab-1250-472b-8f82-ce2842aaf6fe/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 08:32:07,153] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 08:32:07,153] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 08:32:07,153] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 08:32:07,153] [INFO] DFAST Taxonomy check result was written to OceanDNA-b32470/tc_result.tsv
[2023-03-18 08:32:07,153] [INFO] ===== Taxonomy check completed =====
[2023-03-18 08:32:07,153] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 08:32:07,153] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg0c9161ab-1250-472b-8f82-ce2842aaf6fe/dqc_reference/checkm_data
[2023-03-18 08:32:07,156] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 08:32:07,319] [INFO] Task started: CheckM
[2023-03-18 08:32:07,319] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b32470/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b32470/checkm_input OceanDNA-b32470/checkm_result
[2023-03-18 08:32:25,640] [INFO] Task succeeded: CheckM
[2023-03-18 08:32:25,640] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 80.09%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 08:32:25,641] [INFO] ===== Completeness check finished =====
[2023-03-18 08:32:25,641] [INFO] ===== Start GTDB Search =====
[2023-03-18 08:32:25,642] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b32470/markers.fasta)
[2023-03-18 08:32:25,642] [INFO] Task started: Blastn
[2023-03-18 08:32:25,642] [INFO] Running command: blastn -query OceanDNA-b32470/markers.fasta -db /var/lib/cwl/stg0c9161ab-1250-472b-8f82-ce2842aaf6fe/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b32470/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 08:32:26,062] [INFO] Task succeeded: Blastn
[2023-03-18 08:32:26,063] [INFO] Selected 14 target genomes.
[2023-03-18 08:32:26,063] [INFO] Target genome list was writen to OceanDNA-b32470/target_genomes_gtdb.txt
[2023-03-18 08:32:26,083] [INFO] Task started: fastANI
[2023-03-18 08:32:26,083] [INFO] Running command: fastANI --query /var/lib/cwl/stg4ca8a219-ee26-4cb7-997e-db97d982adba/OceanDNA-b32470.fa --refList OceanDNA-b32470/target_genomes_gtdb.txt --output OceanDNA-b32470/fastani_result_gtdb.tsv --threads 1
[2023-03-18 08:32:29,316] [INFO] Task succeeded: fastANI
[2023-03-18 08:32:29,319] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 08:32:29,319] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002715505.1	s__GCA-2715505 sp002715505	77.4643	159	344	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__TMED109;f__TMED131;g__GCA-2715505	95.0	99.85	99.85	0.95	0.95	2	-
GCA_902557845.1	s__GCA-2732015 sp902557845	77.1416	103	344	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__TMED109;f__TMED131;g__GCA-2732015	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 08:32:29,319] [INFO] GTDB search result was written to OceanDNA-b32470/result_gtdb.tsv
[2023-03-18 08:32:29,319] [INFO] ===== GTDB Search completed =====
[2023-03-18 08:32:29,320] [INFO] DFAST_QC result json was written to OceanDNA-b32470/dqc_result.json
[2023-03-18 08:32:29,320] [INFO] DFAST_QC completed!
[2023-03-18 08:32:29,320] [INFO] Total running time: 0h0m33s