[2023-03-18 04:04:53,913] [INFO] DFAST_QC pipeline started.
[2023-03-18 04:04:53,913] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 04:04:53,913] [INFO] DQC Reference Directory: /var/lib/cwl/stg215eb29b-7ac1-46b5-9b65-be7356f59665/dqc_reference
[2023-03-18 04:04:55,026] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 04:04:55,026] [INFO] Task started: Prodigal
[2023-03-18 04:04:55,026] [INFO] Running command: cat /var/lib/cwl/stg61e4bccb-d2c5-4d72-ad22-479c746884e3/OceanDNA-b32667.fa | prodigal -d OceanDNA-b32667/cds.fna -a OceanDNA-b32667/protein.faa -g 11 -q > /dev/null
[2023-03-18 04:04:59,579] [INFO] Task succeeded: Prodigal
[2023-03-18 04:04:59,579] [INFO] Task started: HMMsearch
[2023-03-18 04:04:59,579] [INFO] Running command: hmmsearch --tblout OceanDNA-b32667/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg215eb29b-7ac1-46b5-9b65-be7356f59665/dqc_reference/reference_markers.hmm OceanDNA-b32667/protein.faa > /dev/null
[2023-03-18 04:04:59,728] [INFO] Task succeeded: HMMsearch
[2023-03-18 04:04:59,729] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg61e4bccb-d2c5-4d72-ad22-479c746884e3/OceanDNA-b32667.fa]
[2023-03-18 04:04:59,738] [INFO] Query marker FASTA was written to OceanDNA-b32667/markers.fasta
[2023-03-18 04:04:59,738] [INFO] Task started: Blastn
[2023-03-18 04:04:59,738] [INFO] Running command: blastn -query OceanDNA-b32667/markers.fasta -db /var/lib/cwl/stg215eb29b-7ac1-46b5-9b65-be7356f59665/dqc_reference/reference_markers.fasta -out OceanDNA-b32667/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 04:05:00,302] [INFO] Task succeeded: Blastn
[2023-03-18 04:05:00,302] [INFO] Selected 27 target genomes.
[2023-03-18 04:05:00,303] [INFO] Target genome list was writen to OceanDNA-b32667/target_genomes.txt
[2023-03-18 04:05:00,353] [INFO] Task started: fastANI
[2023-03-18 04:05:00,353] [INFO] Running command: fastANI --query /var/lib/cwl/stg61e4bccb-d2c5-4d72-ad22-479c746884e3/OceanDNA-b32667.fa --refList OceanDNA-b32667/target_genomes.txt --output OceanDNA-b32667/fastani_result.tsv --threads 1
[2023-03-18 04:05:16,841] [INFO] Task succeeded: fastANI
[2023-03-18 04:05:16,841] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg215eb29b-7ac1-46b5-9b65-be7356f59665/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 04:05:16,841] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg215eb29b-7ac1-46b5-9b65-be7356f59665/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 04:05:16,842] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 04:05:16,842] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 04:05:16,842] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 04:05:16,842] [INFO] DFAST Taxonomy check result was written to OceanDNA-b32667/tc_result.tsv
[2023-03-18 04:05:16,842] [INFO] ===== Taxonomy check completed =====
[2023-03-18 04:05:16,842] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 04:05:16,842] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg215eb29b-7ac1-46b5-9b65-be7356f59665/dqc_reference/checkm_data
[2023-03-18 04:05:16,845] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 04:05:16,965] [INFO] Task started: CheckM
[2023-03-18 04:05:16,965] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b32667/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b32667/checkm_input OceanDNA-b32667/checkm_result
[2023-03-18 04:05:34,482] [INFO] Task succeeded: CheckM
[2023-03-18 04:05:34,482] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 72.25%
Contamintation: 0.52%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-18 04:05:34,497] [INFO] ===== Completeness check finished =====
[2023-03-18 04:05:34,497] [INFO] ===== Start GTDB Search =====
[2023-03-18 04:05:34,497] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b32667/markers.fasta)
[2023-03-18 04:05:34,498] [INFO] Task started: Blastn
[2023-03-18 04:05:34,498] [INFO] Running command: blastn -query OceanDNA-b32667/markers.fasta -db /var/lib/cwl/stg215eb29b-7ac1-46b5-9b65-be7356f59665/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b32667/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 04:05:35,404] [INFO] Task succeeded: Blastn
[2023-03-18 04:05:35,412] [INFO] Selected 25 target genomes.
[2023-03-18 04:05:35,412] [INFO] Target genome list was writen to OceanDNA-b32667/target_genomes_gtdb.txt
[2023-03-18 04:05:36,119] [INFO] Task started: fastANI
[2023-03-18 04:05:36,119] [INFO] Running command: fastANI --query /var/lib/cwl/stg61e4bccb-d2c5-4d72-ad22-479c746884e3/OceanDNA-b32667.fa --refList OceanDNA-b32667/target_genomes_gtdb.txt --output OceanDNA-b32667/fastani_result_gtdb.tsv --threads 1
[2023-03-18 04:05:49,208] [INFO] Task succeeded: fastANI
[2023-03-18 04:05:49,210] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 04:05:49,210] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018658465.1	s__JABHVE01 sp018658465	81.0617	178	207	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__CAIVPW01;f__CAIVPW01;g__JABHVE01	95.0	99.60	99.60	0.94	0.94	2	-
--------------------------------------------------------------------------------
[2023-03-18 04:05:49,212] [INFO] GTDB search result was written to OceanDNA-b32667/result_gtdb.tsv
[2023-03-18 04:05:49,214] [INFO] ===== GTDB Search completed =====
[2023-03-18 04:05:49,216] [INFO] DFAST_QC result json was written to OceanDNA-b32667/dqc_result.json
[2023-03-18 04:05:49,216] [INFO] DFAST_QC completed!
[2023-03-18 04:05:49,217] [INFO] Total running time: 0h0m55s