[2023-03-17 11:36:53,836] [INFO] DFAST_QC pipeline started.
[2023-03-17 11:36:53,836] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 11:36:53,836] [INFO] DQC Reference Directory: /var/lib/cwl/stg47ceaf9f-2e3a-4edf-a63a-ef2c4efdba72/dqc_reference
[2023-03-17 11:36:55,024] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 11:36:55,025] [INFO] Task started: Prodigal
[2023-03-17 11:36:55,025] [INFO] Running command: cat /var/lib/cwl/stg740eea37-31df-4a6d-9f99-40c80c00b126/OceanDNA-b32675.fa | prodigal -d OceanDNA-b32675/cds.fna -a OceanDNA-b32675/protein.faa -g 11 -q > /dev/null
[2023-03-17 11:37:03,139] [INFO] Task succeeded: Prodigal
[2023-03-17 11:37:03,139] [INFO] Task started: HMMsearch
[2023-03-17 11:37:03,139] [INFO] Running command: hmmsearch --tblout OceanDNA-b32675/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg47ceaf9f-2e3a-4edf-a63a-ef2c4efdba72/dqc_reference/reference_markers.hmm OceanDNA-b32675/protein.faa > /dev/null
[2023-03-17 11:37:03,301] [INFO] Task succeeded: HMMsearch
[2023-03-17 11:37:03,302] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg740eea37-31df-4a6d-9f99-40c80c00b126/OceanDNA-b32675.fa]
[2023-03-17 11:37:03,319] [INFO] Query marker FASTA was written to OceanDNA-b32675/markers.fasta
[2023-03-17 11:37:03,320] [INFO] Task started: Blastn
[2023-03-17 11:37:03,320] [INFO] Running command: blastn -query OceanDNA-b32675/markers.fasta -db /var/lib/cwl/stg47ceaf9f-2e3a-4edf-a63a-ef2c4efdba72/dqc_reference/reference_markers.fasta -out OceanDNA-b32675/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 11:37:03,970] [INFO] Task succeeded: Blastn
[2023-03-17 11:37:03,973] [INFO] Selected 30 target genomes.
[2023-03-17 11:37:03,974] [INFO] Target genome list was writen to OceanDNA-b32675/target_genomes.txt
[2023-03-17 11:37:03,994] [INFO] Task started: fastANI
[2023-03-17 11:37:03,994] [INFO] Running command: fastANI --query /var/lib/cwl/stg740eea37-31df-4a6d-9f99-40c80c00b126/OceanDNA-b32675.fa --refList OceanDNA-b32675/target_genomes.txt --output OceanDNA-b32675/fastani_result.tsv --threads 1
[2023-03-17 11:37:23,511] [INFO] Task succeeded: fastANI
[2023-03-17 11:37:23,511] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg47ceaf9f-2e3a-4edf-a63a-ef2c4efdba72/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 11:37:23,511] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg47ceaf9f-2e3a-4edf-a63a-ef2c4efdba72/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 11:37:23,511] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 11:37:23,512] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 11:37:23,512] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 11:37:23,513] [INFO] DFAST Taxonomy check result was written to OceanDNA-b32675/tc_result.tsv
[2023-03-17 11:37:23,513] [INFO] ===== Taxonomy check completed =====
[2023-03-17 11:37:23,513] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 11:37:23,513] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg47ceaf9f-2e3a-4edf-a63a-ef2c4efdba72/dqc_reference/checkm_data
[2023-03-17 11:37:23,516] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 11:37:23,566] [INFO] Task started: CheckM
[2023-03-17 11:37:23,566] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b32675/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b32675/checkm_input OceanDNA-b32675/checkm_result
[2023-03-17 11:37:49,142] [INFO] Task succeeded: CheckM
[2023-03-17 11:37:49,143] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 87.50%
Contamintation: 8.33%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-03-17 11:37:49,159] [INFO] ===== Completeness check finished =====
[2023-03-17 11:37:49,160] [INFO] ===== Start GTDB Search =====
[2023-03-17 11:37:49,160] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b32675/markers.fasta)
[2023-03-17 11:37:49,161] [INFO] Task started: Blastn
[2023-03-17 11:37:49,161] [INFO] Running command: blastn -query OceanDNA-b32675/markers.fasta -db /var/lib/cwl/stg47ceaf9f-2e3a-4edf-a63a-ef2c4efdba72/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b32675/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 11:37:50,367] [INFO] Task succeeded: Blastn
[2023-03-17 11:37:50,371] [INFO] Selected 23 target genomes.
[2023-03-17 11:37:50,371] [INFO] Target genome list was writen to OceanDNA-b32675/target_genomes_gtdb.txt
[2023-03-17 11:37:50,390] [INFO] Task started: fastANI
[2023-03-17 11:37:50,390] [INFO] Running command: fastANI --query /var/lib/cwl/stg740eea37-31df-4a6d-9f99-40c80c00b126/OceanDNA-b32675.fa --refList OceanDNA-b32675/target_genomes_gtdb.txt --output OceanDNA-b32675/fastani_result_gtdb.tsv --threads 1
[2023-03-17 11:38:04,498] [INFO] Task succeeded: fastANI
[2023-03-17 11:38:04,500] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-17 11:38:04,500] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018658465.1	s__JABHVE01 sp018658465	82.965	315	408	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__CAIVPW01;f__CAIVPW01;g__JABHVE01	95.0	99.60	99.60	0.94	0.94	2	-
--------------------------------------------------------------------------------
[2023-03-17 11:38:04,501] [INFO] GTDB search result was written to OceanDNA-b32675/result_gtdb.tsv
[2023-03-17 11:38:04,502] [INFO] ===== GTDB Search completed =====
[2023-03-17 11:38:04,503] [INFO] DFAST_QC result json was written to OceanDNA-b32675/dqc_result.json
[2023-03-17 11:38:04,503] [INFO] DFAST_QC completed!
[2023-03-17 11:38:04,503] [INFO] Total running time: 0h1m11s