[2023-03-15 09:48:16,561] [INFO] DFAST_QC pipeline started.
[2023-03-15 09:48:16,561] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 09:48:16,561] [INFO] DQC Reference Directory: /var/lib/cwl/stg98288c6e-3b39-4a2e-8635-0d02fd77cb02/dqc_reference
[2023-03-15 09:48:18,226] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 09:48:18,226] [INFO] Task started: Prodigal
[2023-03-15 09:48:18,226] [INFO] Running command: cat /var/lib/cwl/stg27ce7854-bb24-482d-b653-5b76fc842775/OceanDNA-b34250.fa | prodigal -d OceanDNA-b34250/cds.fna -a OceanDNA-b34250/protein.faa -g 11 -q > /dev/null
[2023-03-15 09:48:20,302] [INFO] Task succeeded: Prodigal
[2023-03-15 09:48:20,302] [INFO] Task started: HMMsearch
[2023-03-15 09:48:20,303] [INFO] Running command: hmmsearch --tblout OceanDNA-b34250/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg98288c6e-3b39-4a2e-8635-0d02fd77cb02/dqc_reference/reference_markers.hmm OceanDNA-b34250/protein.faa > /dev/null
[2023-03-15 09:48:20,536] [INFO] Task succeeded: HMMsearch
[2023-03-15 09:48:20,536] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg27ce7854-bb24-482d-b653-5b76fc842775/OceanDNA-b34250.fa]
[2023-03-15 09:48:20,541] [INFO] Query marker FASTA was written to OceanDNA-b34250/markers.fasta
[2023-03-15 09:48:20,542] [INFO] Task started: Blastn
[2023-03-15 09:48:20,542] [INFO] Running command: blastn -query OceanDNA-b34250/markers.fasta -db /var/lib/cwl/stg98288c6e-3b39-4a2e-8635-0d02fd77cb02/dqc_reference/reference_markers.fasta -out OceanDNA-b34250/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 09:48:24,755] [INFO] Task succeeded: Blastn
[2023-03-15 09:48:24,756] [INFO] Selected 20 target genomes.
[2023-03-15 09:48:24,756] [INFO] Target genome list was writen to OceanDNA-b34250/target_genomes.txt
[2023-03-15 09:48:24,767] [INFO] Task started: fastANI
[2023-03-15 09:48:24,767] [INFO] Running command: fastANI --query /var/lib/cwl/stg27ce7854-bb24-482d-b653-5b76fc842775/OceanDNA-b34250.fa --refList OceanDNA-b34250/target_genomes.txt --output OceanDNA-b34250/fastani_result.tsv --threads 1
[2023-03-15 09:48:34,351] [INFO] Task succeeded: fastANI
[2023-03-15 09:48:34,351] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg98288c6e-3b39-4a2e-8635-0d02fd77cb02/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 09:48:34,352] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg98288c6e-3b39-4a2e-8635-0d02fd77cb02/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 09:48:34,352] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 09:48:34,352] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 09:48:34,352] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 09:48:34,357] [INFO] DFAST Taxonomy check result was written to OceanDNA-b34250/tc_result.tsv
[2023-03-15 09:48:34,357] [INFO] ===== Taxonomy check completed =====
[2023-03-15 09:48:34,357] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 09:48:34,357] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg98288c6e-3b39-4a2e-8635-0d02fd77cb02/dqc_reference/checkm_data
[2023-03-15 09:48:34,360] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 09:48:34,364] [INFO] Task started: CheckM
[2023-03-15 09:48:34,364] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b34250/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b34250/checkm_input OceanDNA-b34250/checkm_result
[2023-03-15 09:48:49,991] [INFO] Task succeeded: CheckM
[2023-03-15 09:48:49,991] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.72%
Contamintation: 4.63%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-03-15 09:48:49,992] [INFO] ===== Completeness check finished =====
[2023-03-15 09:48:49,993] [INFO] ===== Start GTDB Search =====
[2023-03-15 09:48:49,993] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b34250/markers.fasta)
[2023-03-15 09:48:49,994] [INFO] Task started: Blastn
[2023-03-15 09:48:49,994] [INFO] Running command: blastn -query OceanDNA-b34250/markers.fasta -db /var/lib/cwl/stg98288c6e-3b39-4a2e-8635-0d02fd77cb02/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b34250/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 09:48:51,718] [INFO] Task succeeded: Blastn
[2023-03-15 09:48:51,719] [INFO] Selected 25 target genomes.
[2023-03-15 09:48:51,719] [INFO] Target genome list was writen to OceanDNA-b34250/target_genomes_gtdb.txt
[2023-03-15 09:48:52,184] [INFO] Task started: fastANI
[2023-03-15 09:48:52,184] [INFO] Running command: fastANI --query /var/lib/cwl/stg27ce7854-bb24-482d-b653-5b76fc842775/OceanDNA-b34250.fa --refList OceanDNA-b34250/target_genomes_gtdb.txt --output OceanDNA-b34250/fastani_result_gtdb.tsv --threads 1
[2023-03-15 09:49:00,750] [INFO] Task succeeded: fastANI
[2023-03-15 09:49:00,751] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 09:49:00,751] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-15 09:49:00,751] [INFO] GTDB search result was written to OceanDNA-b34250/result_gtdb.tsv
[2023-03-15 09:49:00,751] [INFO] ===== GTDB Search completed =====
[2023-03-15 09:49:00,751] [INFO] DFAST_QC result json was written to OceanDNA-b34250/dqc_result.json
[2023-03-15 09:49:00,751] [INFO] DFAST_QC completed!
[2023-03-15 09:49:00,752] [INFO] Total running time: 0h0m44s