[2023-03-19 03:08:37,663] [INFO] DFAST_QC pipeline started.
[2023-03-19 03:08:37,663] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 03:08:37,663] [INFO] DQC Reference Directory: /var/lib/cwl/stgab95b469-0502-42aa-a78e-05bf66f0bab3/dqc_reference
[2023-03-19 03:08:39,775] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 03:08:39,776] [INFO] Task started: Prodigal
[2023-03-19 03:08:39,776] [INFO] Running command: cat /var/lib/cwl/stgaab24ac0-07f4-46f3-a9ba-52fe5606ed36/OceanDNA-b34254.fa | prodigal -d OceanDNA-b34254/cds.fna -a OceanDNA-b34254/protein.faa -g 11 -q > /dev/null
[2023-03-19 03:08:53,536] [INFO] Task succeeded: Prodigal
[2023-03-19 03:08:53,537] [INFO] Task started: HMMsearch
[2023-03-19 03:08:53,537] [INFO] Running command: hmmsearch --tblout OceanDNA-b34254/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgab95b469-0502-42aa-a78e-05bf66f0bab3/dqc_reference/reference_markers.hmm OceanDNA-b34254/protein.faa > /dev/null
[2023-03-19 03:08:53,865] [INFO] Task succeeded: HMMsearch
[2023-03-19 03:08:53,865] [INFO] Found 6/6 markers.
[2023-03-19 03:08:53,881] [INFO] Query marker FASTA was written to OceanDNA-b34254/markers.fasta
[2023-03-19 03:08:53,882] [INFO] Task started: Blastn
[2023-03-19 03:08:53,882] [INFO] Running command: blastn -query OceanDNA-b34254/markers.fasta -db /var/lib/cwl/stgab95b469-0502-42aa-a78e-05bf66f0bab3/dqc_reference/reference_markers.fasta -out OceanDNA-b34254/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 03:08:54,495] [INFO] Task succeeded: Blastn
[2023-03-19 03:08:54,496] [INFO] Selected 34 target genomes.
[2023-03-19 03:08:54,497] [INFO] Target genome list was writen to OceanDNA-b34254/target_genomes.txt
[2023-03-19 03:08:54,516] [INFO] Task started: fastANI
[2023-03-19 03:08:54,516] [INFO] Running command: fastANI --query /var/lib/cwl/stgaab24ac0-07f4-46f3-a9ba-52fe5606ed36/OceanDNA-b34254.fa --refList OceanDNA-b34254/target_genomes.txt --output OceanDNA-b34254/fastani_result.tsv --threads 1
[2023-03-19 03:09:10,240] [INFO] Task succeeded: fastANI
[2023-03-19 03:09:10,241] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgab95b469-0502-42aa-a78e-05bf66f0bab3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 03:09:10,241] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgab95b469-0502-42aa-a78e-05bf66f0bab3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 03:09:10,241] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-19 03:09:10,241] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-19 03:09:10,241] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-19 03:09:10,241] [INFO] DFAST Taxonomy check result was written to OceanDNA-b34254/tc_result.tsv
[2023-03-19 03:09:10,241] [INFO] ===== Taxonomy check completed =====
[2023-03-19 03:09:10,242] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 03:09:10,242] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgab95b469-0502-42aa-a78e-05bf66f0bab3/dqc_reference/checkm_data
[2023-03-19 03:09:10,294] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 03:09:10,910] [INFO] Task started: CheckM
[2023-03-19 03:09:10,910] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b34254/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b34254/checkm_input OceanDNA-b34254/checkm_result
[2023-03-19 03:09:48,627] [INFO] Task succeeded: CheckM
[2023-03-19 03:09:48,627] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-19 03:09:48,629] [INFO] ===== Completeness check finished =====
[2023-03-19 03:09:48,629] [INFO] ===== Start GTDB Search =====
[2023-03-19 03:09:48,630] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b34254/markers.fasta)
[2023-03-19 03:09:48,631] [INFO] Task started: Blastn
[2023-03-19 03:09:48,631] [INFO] Running command: blastn -query OceanDNA-b34254/markers.fasta -db /var/lib/cwl/stgab95b469-0502-42aa-a78e-05bf66f0bab3/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b34254/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 03:09:49,691] [INFO] Task succeeded: Blastn
[2023-03-19 03:09:49,692] [INFO] Selected 31 target genomes.
[2023-03-19 03:09:49,692] [INFO] Target genome list was writen to OceanDNA-b34254/target_genomes_gtdb.txt
[2023-03-19 03:09:49,707] [INFO] Task started: fastANI
[2023-03-19 03:09:49,708] [INFO] Running command: fastANI --query /var/lib/cwl/stgaab24ac0-07f4-46f3-a9ba-52fe5606ed36/OceanDNA-b34254.fa --refList OceanDNA-b34254/target_genomes_gtdb.txt --output OceanDNA-b34254/fastani_result_gtdb.tsv --threads 1
[2023-03-19 03:10:02,737] [INFO] Task succeeded: fastANI
[2023-03-19 03:10:02,739] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-19 03:10:02,739] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002732805.1	s__GCA-2732805 sp002732805	76.1963	50	846	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Coxiellales;f__Coxiellaceae;g__GCA-2732805	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-19 03:10:02,740] [INFO] GTDB search result was written to OceanDNA-b34254/result_gtdb.tsv
[2023-03-19 03:10:02,740] [INFO] ===== GTDB Search completed =====
[2023-03-19 03:10:02,740] [INFO] DFAST_QC result json was written to OceanDNA-b34254/dqc_result.json
[2023-03-19 03:10:02,740] [INFO] DFAST_QC completed!
[2023-03-19 03:10:02,740] [INFO] Total running time: 0h1m25s