[2023-03-14 10:55:58,932] [INFO] DFAST_QC pipeline started.
[2023-03-14 10:55:58,932] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 10:55:58,932] [INFO] DQC Reference Directory: /var/lib/cwl/stgb279789e-877a-4b33-b014-c2107e0e1346/dqc_reference
[2023-03-14 10:56:00,759] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 10:56:00,759] [INFO] Task started: Prodigal
[2023-03-14 10:56:00,759] [INFO] Running command: cat /var/lib/cwl/stg27f137ef-8a3d-4913-8623-ba86be59a154/OceanDNA-b3463.fa | prodigal -d OceanDNA-b3463/cds.fna -a OceanDNA-b3463/protein.faa -g 11 -q > /dev/null
[2023-03-14 10:56:06,758] [INFO] Task succeeded: Prodigal
[2023-03-14 10:56:06,758] [INFO] Task started: HMMsearch
[2023-03-14 10:56:06,758] [INFO] Running command: hmmsearch --tblout OceanDNA-b3463/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb279789e-877a-4b33-b014-c2107e0e1346/dqc_reference/reference_markers.hmm OceanDNA-b3463/protein.faa > /dev/null
[2023-03-14 10:56:06,924] [INFO] Task succeeded: HMMsearch
[2023-03-14 10:56:06,924] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg27f137ef-8a3d-4913-8623-ba86be59a154/OceanDNA-b3463.fa]
[2023-03-14 10:56:06,934] [INFO] Query marker FASTA was written to OceanDNA-b3463/markers.fasta
[2023-03-14 10:56:06,935] [INFO] Task started: Blastn
[2023-03-14 10:56:06,935] [INFO] Running command: blastn -query OceanDNA-b3463/markers.fasta -db /var/lib/cwl/stgb279789e-877a-4b33-b014-c2107e0e1346/dqc_reference/reference_markers.fasta -out OceanDNA-b3463/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 10:56:07,403] [INFO] Task succeeded: Blastn
[2023-03-14 10:56:07,404] [INFO] Selected 21 target genomes.
[2023-03-14 10:56:07,404] [INFO] Target genome list was writen to OceanDNA-b3463/target_genomes.txt
[2023-03-14 10:56:07,418] [INFO] Task started: fastANI
[2023-03-14 10:56:07,418] [INFO] Running command: fastANI --query /var/lib/cwl/stg27f137ef-8a3d-4913-8623-ba86be59a154/OceanDNA-b3463.fa --refList OceanDNA-b3463/target_genomes.txt --output OceanDNA-b3463/fastani_result.tsv --threads 1
[2023-03-14 10:56:20,665] [INFO] Task succeeded: fastANI
[2023-03-14 10:56:20,665] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb279789e-877a-4b33-b014-c2107e0e1346/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-14 10:56:20,665] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb279789e-877a-4b33-b014-c2107e0e1346/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-14 10:56:20,666] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-14 10:56:20,666] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-14 10:56:20,666] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-14 10:56:20,666] [INFO] DFAST Taxonomy check result was written to OceanDNA-b3463/tc_result.tsv
[2023-03-14 10:56:20,666] [INFO] ===== Taxonomy check completed =====
[2023-03-14 10:56:20,666] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 10:56:20,666] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb279789e-877a-4b33-b014-c2107e0e1346/dqc_reference/checkm_data
[2023-03-14 10:56:20,669] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 10:56:20,671] [INFO] Task started: CheckM
[2023-03-14 10:56:20,672] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b3463/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b3463/checkm_input OceanDNA-b3463/checkm_result
[2023-03-14 10:56:41,864] [INFO] Task succeeded: CheckM
[2023-03-14 10:56:41,865] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 73.35%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-14 10:56:41,867] [INFO] ===== Completeness check finished =====
[2023-03-14 10:56:41,867] [INFO] ===== Start GTDB Search =====
[2023-03-14 10:56:41,867] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b3463/markers.fasta)
[2023-03-14 10:56:41,867] [INFO] Task started: Blastn
[2023-03-14 10:56:41,867] [INFO] Running command: blastn -query OceanDNA-b3463/markers.fasta -db /var/lib/cwl/stgb279789e-877a-4b33-b014-c2107e0e1346/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b3463/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 10:56:42,330] [INFO] Task succeeded: Blastn
[2023-03-14 10:56:42,331] [INFO] Selected 21 target genomes.
[2023-03-14 10:56:42,331] [INFO] Target genome list was writen to OceanDNA-b3463/target_genomes_gtdb.txt
[2023-03-14 10:56:42,355] [INFO] Task started: fastANI
[2023-03-14 10:56:42,355] [INFO] Running command: fastANI --query /var/lib/cwl/stg27f137ef-8a3d-4913-8623-ba86be59a154/OceanDNA-b3463.fa --refList OceanDNA-b3463/target_genomes_gtdb.txt --output OceanDNA-b3463/fastani_result_gtdb.tsv --threads 1
[2023-03-14 10:56:54,181] [INFO] Task succeeded: fastANI
[2023-03-14 10:56:54,183] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-14 10:56:54,183] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_009927275.1	s__UBA2475 sp009927275	99.5157	215	219	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__AKYH767;f__B-17BO;g__UBA2475	95.0	99.68	99.68	0.99	0.99	2	conclusive
--------------------------------------------------------------------------------
[2023-03-14 10:56:54,183] [INFO] GTDB search result was written to OceanDNA-b3463/result_gtdb.tsv
[2023-03-14 10:56:54,184] [INFO] ===== GTDB Search completed =====
[2023-03-14 10:56:54,184] [INFO] DFAST_QC result json was written to OceanDNA-b3463/dqc_result.json
[2023-03-14 10:56:54,184] [INFO] DFAST_QC completed!
[2023-03-14 10:56:54,184] [INFO] Total running time: 0h0m55s