[2023-03-15 05:16:58,353] [INFO] DFAST_QC pipeline started.
[2023-03-15 05:16:58,353] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 05:16:58,353] [INFO] DQC Reference Directory: /var/lib/cwl/stgf8de6559-f7a2-4935-bffe-c542bcf3ee75/dqc_reference
[2023-03-15 05:16:59,443] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 05:16:59,444] [INFO] Task started: Prodigal
[2023-03-15 05:16:59,444] [INFO] Running command: cat /var/lib/cwl/stgedd123ba-010d-466e-b162-7e7eeabf3006/OceanDNA-b357.fa | prodigal -d OceanDNA-b357/cds.fna -a OceanDNA-b357/protein.faa -g 11 -q > /dev/null
[2023-03-15 05:17:05,648] [INFO] Task succeeded: Prodigal
[2023-03-15 05:17:05,649] [INFO] Task started: HMMsearch
[2023-03-15 05:17:05,649] [INFO] Running command: hmmsearch --tblout OceanDNA-b357/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf8de6559-f7a2-4935-bffe-c542bcf3ee75/dqc_reference/reference_markers.hmm OceanDNA-b357/protein.faa > /dev/null
[2023-03-15 05:17:05,794] [INFO] Task succeeded: HMMsearch
[2023-03-15 05:17:05,795] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgedd123ba-010d-466e-b162-7e7eeabf3006/OceanDNA-b357.fa]
[2023-03-15 05:17:05,817] [INFO] Query marker FASTA was written to OceanDNA-b357/markers.fasta
[2023-03-15 05:17:05,819] [INFO] Task started: Blastn
[2023-03-15 05:17:05,819] [INFO] Running command: blastn -query OceanDNA-b357/markers.fasta -db /var/lib/cwl/stgf8de6559-f7a2-4935-bffe-c542bcf3ee75/dqc_reference/reference_markers.fasta -out OceanDNA-b357/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:17:06,312] [INFO] Task succeeded: Blastn
[2023-03-15 05:17:06,324] [INFO] Selected 12 target genomes.
[2023-03-15 05:17:06,324] [INFO] Target genome list was writen to OceanDNA-b357/target_genomes.txt
[2023-03-15 05:17:06,331] [INFO] Task started: fastANI
[2023-03-15 05:17:06,331] [INFO] Running command: fastANI --query /var/lib/cwl/stgedd123ba-010d-466e-b162-7e7eeabf3006/OceanDNA-b357.fa --refList OceanDNA-b357/target_genomes.txt --output OceanDNA-b357/fastani_result.tsv --threads 1
[2023-03-15 05:17:12,873] [INFO] Task succeeded: fastANI
[2023-03-15 05:17:12,873] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf8de6559-f7a2-4935-bffe-c542bcf3ee75/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 05:17:12,873] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf8de6559-f7a2-4935-bffe-c542bcf3ee75/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 05:17:12,874] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 05:17:12,874] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 05:17:12,874] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 05:17:12,875] [INFO] DFAST Taxonomy check result was written to OceanDNA-b357/tc_result.tsv
[2023-03-15 05:17:12,878] [INFO] ===== Taxonomy check completed =====
[2023-03-15 05:17:12,879] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 05:17:12,879] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf8de6559-f7a2-4935-bffe-c542bcf3ee75/dqc_reference/checkm_data
[2023-03-15 05:17:12,882] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 05:17:13,048] [INFO] Task started: CheckM
[2023-03-15 05:17:13,048] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b357/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b357/checkm_input OceanDNA-b357/checkm_result
[2023-03-15 05:17:34,551] [INFO] Task succeeded: CheckM
[2023-03-15 05:17:34,552] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 66.67%
Contamintation: 0.52%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-15 05:17:34,607] [INFO] ===== Completeness check finished =====
[2023-03-15 05:17:34,608] [INFO] ===== Start GTDB Search =====
[2023-03-15 05:17:34,608] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b357/markers.fasta)
[2023-03-15 05:17:34,609] [INFO] Task started: Blastn
[2023-03-15 05:17:34,609] [INFO] Running command: blastn -query OceanDNA-b357/markers.fasta -db /var/lib/cwl/stgf8de6559-f7a2-4935-bffe-c542bcf3ee75/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b357/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:17:35,239] [INFO] Task succeeded: Blastn
[2023-03-15 05:17:35,249] [INFO] Selected 10 target genomes.
[2023-03-15 05:17:35,249] [INFO] Target genome list was writen to OceanDNA-b357/target_genomes_gtdb.txt
[2023-03-15 05:17:35,264] [INFO] Task started: fastANI
[2023-03-15 05:17:35,264] [INFO] Running command: fastANI --query /var/lib/cwl/stgedd123ba-010d-466e-b162-7e7eeabf3006/OceanDNA-b357.fa --refList OceanDNA-b357/target_genomes_gtdb.txt --output OceanDNA-b357/fastani_result_gtdb.tsv --threads 1
[2023-03-15 05:17:37,818] [INFO] Task succeeded: fastANI
[2023-03-15 05:17:37,824] [INFO] Found 9 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 05:17:37,824] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002737635.1	s__BACL27 sp002737635	91.2044	210	321	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_005805045.1	s__BACL27 sp005805045	82.5167	176	321	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	99.25	99.23	0.81	0.81	3	-
GCA_014190055.1	s__BACL27 sp014190055	81.6208	177	321	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	95.53	95.36	0.86	0.77	8	-
GCA_003535455.1	s__BACL27 sp003535455	80.8635	197	321	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	98.51	98.35	0.83	0.76	7	-
GCA_009927565.1	s__BACL27 sp009927565	79.5913	140	321	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_009923685.1	s__BACL27 sp009923685	79.4291	87	321	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016462185.1	s__BACL27 sp016462185	79.0368	166	321	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	98.92	98.92	0.88	0.88	2	-
GCA_014190295.1	s__BACL27 sp014190295	78.0553	137	321	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016463105.1	s__BACL27 sp016463105	77.8672	110	321	d__Bacteria;p__Actinobacteriota;c__Acidimicrobiia;o__Acidimicrobiales;f__Ilumatobacteraceae;g__BACL27	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 05:17:37,826] [INFO] GTDB search result was written to OceanDNA-b357/result_gtdb.tsv
[2023-03-15 05:17:37,828] [INFO] ===== GTDB Search completed =====
[2023-03-15 05:17:37,831] [INFO] DFAST_QC result json was written to OceanDNA-b357/dqc_result.json
[2023-03-15 05:17:37,831] [INFO] DFAST_QC completed!
[2023-03-15 05:17:37,831] [INFO] Total running time: 0h0m39s