[2023-03-15 16:41:11,414] [INFO] DFAST_QC pipeline started.
[2023-03-15 16:41:11,416] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 16:41:11,416] [INFO] DQC Reference Directory: /var/lib/cwl/stg8256ffa1-022e-4bd1-a82a-724f04a9d20a/dqc_reference
[2023-03-15 16:41:13,948] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 16:41:13,949] [INFO] Task started: Prodigal
[2023-03-15 16:41:13,949] [INFO] Running command: cat /var/lib/cwl/stg9a2975a2-36eb-4c83-8d4a-ff40d47a5739/OceanDNA-b36319.fa | prodigal -d OceanDNA-b36319/cds.fna -a OceanDNA-b36319/protein.faa -g 11 -q > /dev/null
[2023-03-15 16:41:27,635] [INFO] Task succeeded: Prodigal
[2023-03-15 16:41:27,635] [INFO] Task started: HMMsearch
[2023-03-15 16:41:27,635] [INFO] Running command: hmmsearch --tblout OceanDNA-b36319/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8256ffa1-022e-4bd1-a82a-724f04a9d20a/dqc_reference/reference_markers.hmm OceanDNA-b36319/protein.faa > /dev/null
[2023-03-15 16:41:27,859] [INFO] Task succeeded: HMMsearch
[2023-03-15 16:41:27,859] [INFO] Found 6/6 markers.
[2023-03-15 16:41:27,885] [INFO] Query marker FASTA was written to OceanDNA-b36319/markers.fasta
[2023-03-15 16:41:27,885] [INFO] Task started: Blastn
[2023-03-15 16:41:27,885] [INFO] Running command: blastn -query OceanDNA-b36319/markers.fasta -db /var/lib/cwl/stg8256ffa1-022e-4bd1-a82a-724f04a9d20a/dqc_reference/reference_markers.fasta -out OceanDNA-b36319/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 16:41:28,501] [INFO] Task succeeded: Blastn
[2023-03-15 16:41:28,503] [INFO] Selected 17 target genomes.
[2023-03-15 16:41:28,503] [INFO] Target genome list was writen to OceanDNA-b36319/target_genomes.txt
[2023-03-15 16:41:28,521] [INFO] Task started: fastANI
[2023-03-15 16:41:28,522] [INFO] Running command: fastANI --query /var/lib/cwl/stg9a2975a2-36eb-4c83-8d4a-ff40d47a5739/OceanDNA-b36319.fa --refList OceanDNA-b36319/target_genomes.txt --output OceanDNA-b36319/fastani_result.tsv --threads 1
[2023-03-15 16:41:38,885] [INFO] Task succeeded: fastANI
[2023-03-15 16:41:38,885] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8256ffa1-022e-4bd1-a82a-724f04a9d20a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 16:41:38,885] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8256ffa1-022e-4bd1-a82a-724f04a9d20a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 16:41:38,891] [INFO] Found 6 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 16:41:38,891] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-15 16:41:38,891] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Methylophaga nitratireducenticrescens	strain=JAM1	GCA_000260985.3	754476	754476	type	True	90.3625	620	785	95	below_threshold
Methylophaga pinxianii	strain=TMB456	GCA_020447225.1	2881052	2881052	type	True	82.0485	518	785	95	below_threshold
Methylophaga frappieri	strain=JAM7	GCA_000260965.1	754477	754477	type	True	77.4391	76	785	95	below_threshold
Methylophaga sulfidovorans	strain=DSM 11578	GCA_900114205.1	45496	45496	type	True	76.9404	98	785	95	below_threshold
Methylophaga thiooxydans	strain=DMS010	GCA_000156355.1	392484	392484	type	True	76.8362	88	785	95	below_threshold
Methylophaga aminisulfidivorans	strain=MP	GCA_000214595.2	230105	230105	type	True	76.7827	99	785	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-15 16:41:38,893] [INFO] DFAST Taxonomy check result was written to OceanDNA-b36319/tc_result.tsv
[2023-03-15 16:41:38,896] [INFO] ===== Taxonomy check completed =====
[2023-03-15 16:41:38,896] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 16:41:38,896] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8256ffa1-022e-4bd1-a82a-724f04a9d20a/dqc_reference/checkm_data
[2023-03-15 16:41:38,897] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 16:41:38,947] [INFO] Task started: CheckM
[2023-03-15 16:41:38,947] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b36319/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b36319/checkm_input OceanDNA-b36319/checkm_result
[2023-03-15 16:42:17,337] [INFO] Task succeeded: CheckM
[2023-03-15 16:42:17,338] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.02%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 16:42:17,346] [INFO] ===== Completeness check finished =====
[2023-03-15 16:42:17,346] [INFO] ===== Start GTDB Search =====
[2023-03-15 16:42:17,346] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b36319/markers.fasta)
[2023-03-15 16:42:17,347] [INFO] Task started: Blastn
[2023-03-15 16:42:17,347] [INFO] Running command: blastn -query OceanDNA-b36319/markers.fasta -db /var/lib/cwl/stg8256ffa1-022e-4bd1-a82a-724f04a9d20a/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b36319/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 16:42:18,105] [INFO] Task succeeded: Blastn
[2023-03-15 16:42:18,106] [INFO] Selected 5 target genomes.
[2023-03-15 16:42:18,106] [INFO] Target genome list was writen to OceanDNA-b36319/target_genomes_gtdb.txt
[2023-03-15 16:42:18,111] [INFO] Task started: fastANI
[2023-03-15 16:42:18,112] [INFO] Running command: fastANI --query /var/lib/cwl/stg9a2975a2-36eb-4c83-8d4a-ff40d47a5739/OceanDNA-b36319.fa --refList OceanDNA-b36319/target_genomes_gtdb.txt --output OceanDNA-b36319/fastani_result_gtdb.tsv --threads 1
[2023-03-15 16:42:21,677] [INFO] Task succeeded: fastANI
[2023-03-15 16:42:21,681] [INFO] Found 5 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 16:42:21,681] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002354555.1	s__Methylophaga sp002354555	99.8055	699	785	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Nitrosococcales;f__Methylophagaceae;g__Methylophaga	95.0	98.87	98.17	0.91	0.88	3	conclusive
GCA_002696735.1	s__Methylophaga sp002696735	91.6118	705	785	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Nitrosococcales;f__Methylophagaceae;g__Methylophaga	95.0	98.51	97.89	0.93	0.90	20	-
GCF_000260985.4	s__Methylophaga nitratireducenticrescens	90.3625	620	785	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Nitrosococcales;f__Methylophagaceae;g__Methylophaga	95.0	99.21	99.21	0.92	0.92	2	-
GCA_002363955.1	s__Methylophaga sp002363955	83.1021	554	785	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Nitrosococcales;f__Methylophagaceae;g__Methylophaga	95.0	98.95	98.64	0.94	0.91	14	-
GCF_001720165.1	s__Methylophaga muralis	82.5416	512	785	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Nitrosococcales;f__Methylophagaceae;g__Methylophaga	95.0	97.83	97.83	0.96	0.96	2	-
--------------------------------------------------------------------------------
[2023-03-15 16:42:21,682] [INFO] GTDB search result was written to OceanDNA-b36319/result_gtdb.tsv
[2023-03-15 16:42:21,682] [INFO] ===== GTDB Search completed =====
[2023-03-15 16:42:21,684] [INFO] DFAST_QC result json was written to OceanDNA-b36319/dqc_result.json
[2023-03-15 16:42:21,684] [INFO] DFAST_QC completed!
[2023-03-15 16:42:21,684] [INFO] Total running time: 0h1m10s