[2023-03-14 12:25:41,527] [INFO] DFAST_QC pipeline started.
[2023-03-14 12:25:41,527] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 12:25:41,527] [INFO] DQC Reference Directory: /var/lib/cwl/stg8f4e5e0d-3065-4f0f-8ca7-3fe4fb9c217a/dqc_reference
[2023-03-14 12:25:42,733] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 12:25:42,733] [INFO] Task started: Prodigal
[2023-03-14 12:25:42,733] [INFO] Running command: cat /var/lib/cwl/stgd75f6d56-e122-4cf3-84a7-8da5d91ef989/OceanDNA-b3645.fa | prodigal -d OceanDNA-b3645/cds.fna -a OceanDNA-b3645/protein.faa -g 11 -q > /dev/null
[2023-03-14 12:25:59,486] [INFO] Task succeeded: Prodigal
[2023-03-14 12:25:59,486] [INFO] Task started: HMMsearch
[2023-03-14 12:25:59,486] [INFO] Running command: hmmsearch --tblout OceanDNA-b3645/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8f4e5e0d-3065-4f0f-8ca7-3fe4fb9c217a/dqc_reference/reference_markers.hmm OceanDNA-b3645/protein.faa > /dev/null
[2023-03-14 12:25:59,692] [INFO] Task succeeded: HMMsearch
[2023-03-14 12:25:59,692] [INFO] Found 6/6 markers.
[2023-03-14 12:25:59,709] [INFO] Query marker FASTA was written to OceanDNA-b3645/markers.fasta
[2023-03-14 12:25:59,710] [INFO] Task started: Blastn
[2023-03-14 12:25:59,710] [INFO] Running command: blastn -query OceanDNA-b3645/markers.fasta -db /var/lib/cwl/stg8f4e5e0d-3065-4f0f-8ca7-3fe4fb9c217a/dqc_reference/reference_markers.fasta -out OceanDNA-b3645/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 12:26:00,237] [INFO] Task succeeded: Blastn
[2023-03-14 12:26:00,238] [INFO] Selected 25 target genomes.
[2023-03-14 12:26:00,238] [INFO] Target genome list was writen to OceanDNA-b3645/target_genomes.txt
[2023-03-14 12:26:00,256] [INFO] Task started: fastANI
[2023-03-14 12:26:00,256] [INFO] Running command: fastANI --query /var/lib/cwl/stgd75f6d56-e122-4cf3-84a7-8da5d91ef989/OceanDNA-b3645.fa --refList OceanDNA-b3645/target_genomes.txt --output OceanDNA-b3645/fastani_result.tsv --threads 1
[2023-03-14 12:26:18,352] [INFO] Task succeeded: fastANI
[2023-03-14 12:26:18,352] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8f4e5e0d-3065-4f0f-8ca7-3fe4fb9c217a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-14 12:26:18,352] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8f4e5e0d-3065-4f0f-8ca7-3fe4fb9c217a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-14 12:26:18,352] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-14 12:26:18,353] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-14 12:26:18,353] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-14 12:26:18,353] [INFO] DFAST Taxonomy check result was written to OceanDNA-b3645/tc_result.tsv
[2023-03-14 12:26:18,353] [INFO] ===== Taxonomy check completed =====
[2023-03-14 12:26:18,353] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 12:26:18,353] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8f4e5e0d-3065-4f0f-8ca7-3fe4fb9c217a/dqc_reference/checkm_data
[2023-03-14 12:26:18,355] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 12:26:18,397] [INFO] Task started: CheckM
[2023-03-14 12:26:18,397] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b3645/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b3645/checkm_input OceanDNA-b3645/checkm_result
[2023-03-14 12:27:04,665] [INFO] Task succeeded: CheckM
[2023-03-14 12:27:04,665] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 84.45%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-14 12:27:04,687] [INFO] ===== Completeness check finished =====
[2023-03-14 12:27:04,687] [INFO] ===== Start GTDB Search =====
[2023-03-14 12:27:04,687] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b3645/markers.fasta)
[2023-03-14 12:27:04,688] [INFO] Task started: Blastn
[2023-03-14 12:27:04,689] [INFO] Running command: blastn -query OceanDNA-b3645/markers.fasta -db /var/lib/cwl/stg8f4e5e0d-3065-4f0f-8ca7-3fe4fb9c217a/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b3645/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 12:27:05,403] [INFO] Task succeeded: Blastn
[2023-03-14 12:27:05,404] [INFO] Selected 29 target genomes.
[2023-03-14 12:27:05,404] [INFO] Target genome list was writen to OceanDNA-b3645/target_genomes_gtdb.txt
[2023-03-14 12:27:05,428] [INFO] Task started: fastANI
[2023-03-14 12:27:05,428] [INFO] Running command: fastANI --query /var/lib/cwl/stgd75f6d56-e122-4cf3-84a7-8da5d91ef989/OceanDNA-b3645.fa --refList OceanDNA-b3645/target_genomes_gtdb.txt --output OceanDNA-b3645/fastani_result_gtdb.tsv --threads 1
[2023-03-14 12:27:27,839] [INFO] Task succeeded: fastANI
[2023-03-14 12:27:27,839] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-14 12:27:27,840] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-14 12:27:27,840] [INFO] GTDB search result was written to OceanDNA-b3645/result_gtdb.tsv
[2023-03-14 12:27:27,840] [INFO] ===== GTDB Search completed =====
[2023-03-14 12:27:27,840] [INFO] DFAST_QC result json was written to OceanDNA-b3645/dqc_result.json
[2023-03-14 12:27:27,840] [INFO] DFAST_QC completed!
[2023-03-14 12:27:27,840] [INFO] Total running time: 0h1m46s