[2023-03-16 06:12:59,354] [INFO] DFAST_QC pipeline started.
[2023-03-16 06:12:59,355] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 06:12:59,355] [INFO] DQC Reference Directory: /var/lib/cwl/stg196ea9eb-afd2-4d66-86f9-465c12a94d24/dqc_reference
[2023-03-16 06:13:00,450] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 06:13:00,450] [INFO] Task started: Prodigal
[2023-03-16 06:13:00,450] [INFO] Running command: cat /var/lib/cwl/stg64cb10c2-c1bb-4294-8077-9e3b33a752f8/OceanDNA-b36456.fa | prodigal -d OceanDNA-b36456/cds.fna -a OceanDNA-b36456/protein.faa -g 11 -q > /dev/null
[2023-03-16 06:13:24,166] [INFO] Task succeeded: Prodigal
[2023-03-16 06:13:24,166] [INFO] Task started: HMMsearch
[2023-03-16 06:13:24,166] [INFO] Running command: hmmsearch --tblout OceanDNA-b36456/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg196ea9eb-afd2-4d66-86f9-465c12a94d24/dqc_reference/reference_markers.hmm OceanDNA-b36456/protein.faa > /dev/null
[2023-03-16 06:13:24,382] [INFO] Task succeeded: HMMsearch
[2023-03-16 06:13:24,382] [INFO] Found 6/6 markers.
[2023-03-16 06:13:24,405] [INFO] Query marker FASTA was written to OceanDNA-b36456/markers.fasta
[2023-03-16 06:13:24,407] [INFO] Task started: Blastn
[2023-03-16 06:13:24,407] [INFO] Running command: blastn -query OceanDNA-b36456/markers.fasta -db /var/lib/cwl/stg196ea9eb-afd2-4d66-86f9-465c12a94d24/dqc_reference/reference_markers.fasta -out OceanDNA-b36456/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 06:13:25,136] [INFO] Task succeeded: Blastn
[2023-03-16 06:13:25,137] [INFO] Selected 13 target genomes.
[2023-03-16 06:13:25,138] [INFO] Target genome list was writen to OceanDNA-b36456/target_genomes.txt
[2023-03-16 06:13:25,146] [INFO] Task started: fastANI
[2023-03-16 06:13:25,146] [INFO] Running command: fastANI --query /var/lib/cwl/stg64cb10c2-c1bb-4294-8077-9e3b33a752f8/OceanDNA-b36456.fa --refList OceanDNA-b36456/target_genomes.txt --output OceanDNA-b36456/fastani_result.tsv --threads 1
[2023-03-16 06:13:35,177] [INFO] Task succeeded: fastANI
[2023-03-16 06:13:35,177] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg196ea9eb-afd2-4d66-86f9-465c12a94d24/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 06:13:35,178] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg196ea9eb-afd2-4d66-86f9-465c12a94d24/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 06:13:35,186] [INFO] Found 13 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 06:13:35,186] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-16 06:13:35,186] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Alcanivorax borkumensis	strain=SK2	GCA_000009365.1	59754	59754	type	True	83.055	789	1244	95	below_threshold
Alcanivorax jadensis	strain=T9	GCA_000756655.1	64988	64988	type	True	82.2157	801	1244	95	below_threshold
Alcanivorax profundi	strain=MTEO17	GCA_003597125.1	2338368	2338368	type	True	81.1457	686	1244	95	below_threshold
Alcanivorax hongdengensis	strain=A-11-3	GCA_000300995.1	519051	519051	type	True	81.0897	686	1244	95	below_threshold
Alcanivorax nanhaiticus	strain=19-m-6	GCA_000756665.1	1177154	1177154	type	True	80.7458	666	1244	95	below_threshold
Alcanivorax sediminis	strain=PA15-N-34	GCA_009601165.1	2663008	2663008	type	True	80.7023	639	1244	95	below_threshold
Alcanivorax marinus	strain=R8-12	GCA_025532125.1	1177169	1177169	type	True	78.9186	421	1244	95	below_threshold
Alcanivorax balearicus	strain=MACL04	GCA_025532145.1	413232	413232	type	True	78.7243	400	1244	95	below_threshold
Alcanivorax limicola	strain=JB21	GCA_019931215.1	2874102	2874102	type	True	77.9291	257	1244	95	below_threshold
Pseudomonas capeferrum	strain=WCS358	GCA_022548835.1	1495066	1495066	type	True	76.7198	81	1244	95	below_threshold
Pseudomonas hydrolytica	strain=DSWY01	GCA_021495345.2	2493633	2493633	type	True	76.678	142	1244	95	below_threshold
Pseudomonas sagittaria	strain=JCM 18195	GCA_900115715.1	1135990	1135990	type	True	76.5388	115	1244	95	below_threshold
Pseudomonas mucidolens	strain=NCTC8068	GCA_900475945.1	46679	46679	type	True	76.1575	85	1244	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-16 06:13:35,187] [INFO] DFAST Taxonomy check result was written to OceanDNA-b36456/tc_result.tsv
[2023-03-16 06:13:35,188] [INFO] ===== Taxonomy check completed =====
[2023-03-16 06:13:35,188] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 06:13:35,188] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg196ea9eb-afd2-4d66-86f9-465c12a94d24/dqc_reference/checkm_data
[2023-03-16 06:13:35,188] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 06:13:35,194] [INFO] Task started: CheckM
[2023-03-16 06:13:35,194] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b36456/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b36456/checkm_input OceanDNA-b36456/checkm_result
[2023-03-16 06:14:34,412] [INFO] Task succeeded: CheckM
[2023-03-16 06:14:34,412] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 06:14:34,431] [INFO] ===== Completeness check finished =====
[2023-03-16 06:14:34,432] [INFO] ===== Start GTDB Search =====
[2023-03-16 06:14:34,432] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b36456/markers.fasta)
[2023-03-16 06:14:34,433] [INFO] Task started: Blastn
[2023-03-16 06:14:34,433] [INFO] Running command: blastn -query OceanDNA-b36456/markers.fasta -db /var/lib/cwl/stg196ea9eb-afd2-4d66-86f9-465c12a94d24/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b36456/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 06:14:35,724] [INFO] Task succeeded: Blastn
[2023-03-16 06:14:35,725] [INFO] Selected 10 target genomes.
[2023-03-16 06:14:35,725] [INFO] Target genome list was writen to OceanDNA-b36456/target_genomes_gtdb.txt
[2023-03-16 06:14:35,757] [INFO] Task started: fastANI
[2023-03-16 06:14:35,757] [INFO] Running command: fastANI --query /var/lib/cwl/stg64cb10c2-c1bb-4294-8077-9e3b33a752f8/OceanDNA-b36456.fa --refList OceanDNA-b36456/target_genomes_gtdb.txt --output OceanDNA-b36456/fastani_result_gtdb.tsv --threads 1
[2023-03-16 06:14:45,014] [INFO] Task succeeded: fastANI
[2023-03-16 06:14:45,021] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-16 06:14:45,021] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000155615.1	s__Alcanivorax sp000155615	99.6419	1210	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	97.88	97.69	0.92	0.89	4	conclusive
GCA_002726155.1	s__Alcanivorax sp002726155	92.8693	1056	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002733835.1	s__Alcanivorax sp002733835	92.3934	999	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000009365.1	s__Alcanivorax borkumensis	83.055	789	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	99.54	99.30	0.96	0.95	6	-
GCA_003862375.1	s__Alcanivorax jadensis_A	82.3546	773	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	99.67	99.67	0.92	0.92	2	-
GCF_900107995.1	s__Alcanivorax sp900107995	82.2463	763	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	98.42	98.03	0.93	0.89	9	-
GCF_000756655.1	s__Alcanivorax jadensis	82.2299	798	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	98.11	97.73	0.92	0.86	7	-
GCA_017794965.1	s__Alcanivorax sp017794965	81.1436	710	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000300995.1	s__Alcanivorax hongdengensis	81.0957	685	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009601165.1	s__Alcanivorax sediminis	80.7084	638	1244	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 06:14:45,022] [INFO] GTDB search result was written to OceanDNA-b36456/result_gtdb.tsv
[2023-03-16 06:14:45,023] [INFO] ===== GTDB Search completed =====
[2023-03-16 06:14:45,025] [INFO] DFAST_QC result json was written to OceanDNA-b36456/dqc_result.json
[2023-03-16 06:14:45,025] [INFO] DFAST_QC completed!
[2023-03-16 06:14:45,025] [INFO] Total running time: 0h1m46s