[2023-03-19 02:59:38,308] [INFO] DFAST_QC pipeline started.
[2023-03-19 02:59:38,308] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 02:59:38,308] [INFO] DQC Reference Directory: /var/lib/cwl/stgd0219c84-3cd1-4604-8eeb-7689e3cec4b4/dqc_reference
[2023-03-19 02:59:39,408] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 02:59:39,408] [INFO] Task started: Prodigal
[2023-03-19 02:59:39,408] [INFO] Running command: cat /var/lib/cwl/stg69e2068c-0a3e-478d-bf0d-5d8bbdae93c0/OceanDNA-b36473.fa | prodigal -d OceanDNA-b36473/cds.fna -a OceanDNA-b36473/protein.faa -g 11 -q > /dev/null
[2023-03-19 03:00:05,173] [INFO] Task succeeded: Prodigal
[2023-03-19 03:00:05,173] [INFO] Task started: HMMsearch
[2023-03-19 03:00:05,173] [INFO] Running command: hmmsearch --tblout OceanDNA-b36473/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd0219c84-3cd1-4604-8eeb-7689e3cec4b4/dqc_reference/reference_markers.hmm OceanDNA-b36473/protein.faa > /dev/null
[2023-03-19 03:00:05,388] [INFO] Task succeeded: HMMsearch
[2023-03-19 03:00:05,389] [INFO] Found 6/6 markers.
[2023-03-19 03:00:05,414] [INFO] Query marker FASTA was written to OceanDNA-b36473/markers.fasta
[2023-03-19 03:00:05,414] [INFO] Task started: Blastn
[2023-03-19 03:00:05,414] [INFO] Running command: blastn -query OceanDNA-b36473/markers.fasta -db /var/lib/cwl/stgd0219c84-3cd1-4604-8eeb-7689e3cec4b4/dqc_reference/reference_markers.fasta -out OceanDNA-b36473/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 03:00:06,302] [INFO] Task succeeded: Blastn
[2023-03-19 03:00:06,303] [INFO] Selected 11 target genomes.
[2023-03-19 03:00:06,303] [INFO] Target genome list was writen to OceanDNA-b36473/target_genomes.txt
[2023-03-19 03:00:06,309] [INFO] Task started: fastANI
[2023-03-19 03:00:06,309] [INFO] Running command: fastANI --query /var/lib/cwl/stg69e2068c-0a3e-478d-bf0d-5d8bbdae93c0/OceanDNA-b36473.fa --refList OceanDNA-b36473/target_genomes.txt --output OceanDNA-b36473/fastani_result.tsv --threads 1
[2023-03-19 03:00:16,447] [INFO] Task succeeded: fastANI
[2023-03-19 03:00:16,447] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd0219c84-3cd1-4604-8eeb-7689e3cec4b4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 03:00:16,448] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd0219c84-3cd1-4604-8eeb-7689e3cec4b4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 03:00:16,455] [INFO] Found 11 fastANI hits (1 hits with ANI > threshold)
[2023-03-19 03:00:16,455] [INFO] The taxonomy check result is classified as 'conclusive'.
[2023-03-19 03:00:16,455] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Alcanivorax profundimaris	strain=ST75FaO-1	GCA_015265435.1	2735259	2735259	type	True	97.476	1118	1364	95	conclusive
Alcanivorax marinus	strain=R8-12	GCA_025532125.1	1177169	1177169	type	True	88.1309	903	1364	95	below_threshold
Alcanivorax gelatiniphagus	strain=MEBiC 08158	GCA_005938655.1	1194167	1194167	type	True	87.9132	997	1364	95	below_threshold
Alcanivorax venustensis	strain=ISO4	GCA_015356855.1	172371	172371	type	True	85.6293	827	1364	95	below_threshold
Alcanivorax mobilis	strain=MT13131	GCA_002864685.1	2019569	2019569	type	True	82.4789	763	1364	95	below_threshold
Alcanivorax dieselolei	strain=CGMCC 1.3690	GCA_014644575.1	285091	285091	type	True	81.1075	756	1364	95	below_threshold
Alcanivorax balearicus	strain=MACL04	GCA_025532145.1	413232	413232	type	True	80.8659	708	1364	95	below_threshold
Alcanivorax xenomutans	strain=JC109	GCA_900217905.1	1094342	1094342	type	True	80.4785	680	1364	95	below_threshold
Alcanivorax limicola	strain=JB21	GCA_019931215.1	2874102	2874102	type	True	78.0912	291	1364	95	below_threshold
Pseudomonas aromaticivorans	strain=MAP12	GCA_019097855.1	2849492	2849492	type	True	77.6918	303	1364	95	below_threshold
Pseudomonas lalucatii	strain=R1b54	GCA_018398425.1	1424203	1424203	type	True	77.6323	340	1364	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-19 03:00:16,455] [INFO] DFAST Taxonomy check result was written to OceanDNA-b36473/tc_result.tsv
[2023-03-19 03:00:16,455] [INFO] ===== Taxonomy check completed =====
[2023-03-19 03:00:16,455] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 03:00:16,456] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd0219c84-3cd1-4604-8eeb-7689e3cec4b4/dqc_reference/checkm_data
[2023-03-19 03:00:16,456] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 03:00:16,462] [INFO] Task started: CheckM
[2023-03-19 03:00:16,462] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b36473/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b36473/checkm_input OceanDNA-b36473/checkm_result
[2023-03-19 03:01:19,688] [INFO] Task succeeded: CheckM
[2023-03-19 03:01:19,689] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-19 03:01:19,691] [INFO] ===== Completeness check finished =====
[2023-03-19 03:01:19,691] [INFO] ===== Start GTDB Search =====
[2023-03-19 03:01:19,692] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b36473/markers.fasta)
[2023-03-19 03:01:19,692] [INFO] Task started: Blastn
[2023-03-19 03:01:19,692] [INFO] Running command: blastn -query OceanDNA-b36473/markers.fasta -db /var/lib/cwl/stgd0219c84-3cd1-4604-8eeb-7689e3cec4b4/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b36473/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 03:01:21,432] [INFO] Task succeeded: Blastn
[2023-03-19 03:01:21,433] [INFO] Selected 7 target genomes.
[2023-03-19 03:01:21,433] [INFO] Target genome list was writen to OceanDNA-b36473/target_genomes_gtdb.txt
[2023-03-19 03:01:21,440] [INFO] Task started: fastANI
[2023-03-19 03:01:21,440] [INFO] Running command: fastANI --query /var/lib/cwl/stg69e2068c-0a3e-478d-bf0d-5d8bbdae93c0/OceanDNA-b36473.fa --refList OceanDNA-b36473/target_genomes_gtdb.txt --output OceanDNA-b36473/fastani_result_gtdb.tsv --threads 1
[2023-03-19 03:01:28,360] [INFO] Task succeeded: fastANI
[2023-03-19 03:01:28,366] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-19 03:01:28,366] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_015265435.1	s__Alcanivorax profundimaris	97.5198	1116	1364	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	97.49	97.23	0.85	0.77	11	conclusive
GCF_016785095.1	s__Alcanivorax marinus_A	91.1297	1052	1364	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016906305.1	s__Alcanivorax marinus_B	88.1296	989	1364	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	N/A	N/A	N/A	N/A	1	-
GCF_005938655.1	s__Alcanivorax gelatiniphagus	87.9151	996	1364	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	98.53	98.53	0.93	0.93	3	-
GCF_015356855.1	s__Alcanivorax venustensis	85.6531	825	1364	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	97.73	95.38	0.91	0.87	46	-
GCA_017794945.1	s__Alcanivorax sp009711585	85.1324	754	1364	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	96.99	96.99	0.93	0.93	2	-
GCF_004360225.1	s__Alcanivorax sp004360225	80.2961	669	1364	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	99.14	99.14	0.91	0.91	2	-
--------------------------------------------------------------------------------
[2023-03-19 03:01:28,366] [INFO] GTDB search result was written to OceanDNA-b36473/result_gtdb.tsv
[2023-03-19 03:01:28,366] [INFO] ===== GTDB Search completed =====
[2023-03-19 03:01:28,367] [INFO] DFAST_QC result json was written to OceanDNA-b36473/dqc_result.json
[2023-03-19 03:01:28,367] [INFO] DFAST_QC completed!
[2023-03-19 03:01:28,367] [INFO] Total running time: 0h1m50s