[2023-03-14 12:13:42,665] [INFO] DFAST_QC pipeline started.
[2023-03-14 12:13:42,665] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 12:13:42,665] [INFO] DQC Reference Directory: /var/lib/cwl/stg8c0e5970-a3df-47e1-8f57-a2dafb147b42/dqc_reference
[2023-03-14 12:13:44,622] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 12:13:44,622] [INFO] Task started: Prodigal
[2023-03-14 12:13:44,622] [INFO] Running command: cat /var/lib/cwl/stgbbd9817c-4399-4a1a-8bde-8d9d01bfbe64/OceanDNA-b36655.fa | prodigal -d OceanDNA-b36655/cds.fna -a OceanDNA-b36655/protein.faa -g 11 -q > /dev/null
[2023-03-14 12:13:50,484] [INFO] Task succeeded: Prodigal
[2023-03-14 12:13:50,484] [INFO] Task started: HMMsearch
[2023-03-14 12:13:50,484] [INFO] Running command: hmmsearch --tblout OceanDNA-b36655/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8c0e5970-a3df-47e1-8f57-a2dafb147b42/dqc_reference/reference_markers.hmm OceanDNA-b36655/protein.faa > /dev/null
[2023-03-14 12:13:50,642] [INFO] Task succeeded: HMMsearch
[2023-03-14 12:13:50,643] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgbbd9817c-4399-4a1a-8bde-8d9d01bfbe64/OceanDNA-b36655.fa]
[2023-03-14 12:13:50,653] [INFO] Query marker FASTA was written to OceanDNA-b36655/markers.fasta
[2023-03-14 12:13:50,653] [INFO] Task started: Blastn
[2023-03-14 12:13:50,653] [INFO] Running command: blastn -query OceanDNA-b36655/markers.fasta -db /var/lib/cwl/stg8c0e5970-a3df-47e1-8f57-a2dafb147b42/dqc_reference/reference_markers.fasta -out OceanDNA-b36655/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 12:13:51,227] [INFO] Task succeeded: Blastn
[2023-03-14 12:13:51,228] [INFO] Selected 21 target genomes.
[2023-03-14 12:13:51,228] [INFO] Target genome list was writen to OceanDNA-b36655/target_genomes.txt
[2023-03-14 12:13:51,242] [INFO] Task started: fastANI
[2023-03-14 12:13:51,243] [INFO] Running command: fastANI --query /var/lib/cwl/stgbbd9817c-4399-4a1a-8bde-8d9d01bfbe64/OceanDNA-b36655.fa --refList OceanDNA-b36655/target_genomes.txt --output OceanDNA-b36655/fastani_result.tsv --threads 1
[2023-03-14 12:14:04,631] [INFO] Task succeeded: fastANI
[2023-03-14 12:14:04,631] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8c0e5970-a3df-47e1-8f57-a2dafb147b42/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-14 12:14:04,631] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8c0e5970-a3df-47e1-8f57-a2dafb147b42/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-14 12:14:04,632] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-14 12:14:04,632] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-14 12:14:04,632] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-14 12:14:04,632] [INFO] DFAST Taxonomy check result was written to OceanDNA-b36655/tc_result.tsv
[2023-03-14 12:14:04,632] [INFO] ===== Taxonomy check completed =====
[2023-03-14 12:14:04,632] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 12:14:04,632] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8c0e5970-a3df-47e1-8f57-a2dafb147b42/dqc_reference/checkm_data
[2023-03-14 12:14:04,635] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 12:14:04,638] [INFO] Task started: CheckM
[2023-03-14 12:14:04,638] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b36655/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b36655/checkm_input OceanDNA-b36655/checkm_result
[2023-03-14 12:14:25,250] [INFO] Task succeeded: CheckM
[2023-03-14 12:14:25,250] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 87.50%
Contamintation: 6.71%
Strain heterogeneity: 66.67%
--------------------------------------------------------------------------------
[2023-03-14 12:14:25,255] [INFO] ===== Completeness check finished =====
[2023-03-14 12:14:25,255] [INFO] ===== Start GTDB Search =====
[2023-03-14 12:14:25,255] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b36655/markers.fasta)
[2023-03-14 12:14:25,255] [INFO] Task started: Blastn
[2023-03-14 12:14:25,255] [INFO] Running command: blastn -query OceanDNA-b36655/markers.fasta -db /var/lib/cwl/stg8c0e5970-a3df-47e1-8f57-a2dafb147b42/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b36655/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 12:14:26,206] [INFO] Task succeeded: Blastn
[2023-03-14 12:14:26,208] [INFO] Selected 29 target genomes.
[2023-03-14 12:14:26,208] [INFO] Target genome list was writen to OceanDNA-b36655/target_genomes_gtdb.txt
[2023-03-14 12:14:26,236] [INFO] Task started: fastANI
[2023-03-14 12:14:26,237] [INFO] Running command: fastANI --query /var/lib/cwl/stgbbd9817c-4399-4a1a-8bde-8d9d01bfbe64/OceanDNA-b36655.fa --refList OceanDNA-b36655/target_genomes_gtdb.txt --output OceanDNA-b36655/fastani_result_gtdb.tsv --threads 1
[2023-03-14 12:14:43,042] [INFO] Task succeeded: fastANI
[2023-03-14 12:14:43,043] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-14 12:14:43,043] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-14 12:14:43,044] [INFO] GTDB search result was written to OceanDNA-b36655/result_gtdb.tsv
[2023-03-14 12:14:43,044] [INFO] ===== GTDB Search completed =====
[2023-03-14 12:14:43,045] [INFO] DFAST_QC result json was written to OceanDNA-b36655/dqc_result.json
[2023-03-14 12:14:43,045] [INFO] DFAST_QC completed!
[2023-03-14 12:14:43,045] [INFO] Total running time: 0h1m0s