[2023-03-19 01:00:39,672] [INFO] DFAST_QC pipeline started.
[2023-03-19 01:00:39,672] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 01:00:39,672] [INFO] DQC Reference Directory: /var/lib/cwl/stgc31185ba-4432-4ade-805a-13fa1632801c/dqc_reference
[2023-03-19 01:00:40,752] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 01:00:40,753] [INFO] Task started: Prodigal
[2023-03-19 01:00:40,753] [INFO] Running command: cat /var/lib/cwl/stg8b29f4f1-0e63-4385-ab63-a9c8932d23f5/OceanDNA-b36678.fa | prodigal -d OceanDNA-b36678/cds.fna -a OceanDNA-b36678/protein.faa -g 11 -q > /dev/null
[2023-03-19 01:00:55,424] [INFO] Task succeeded: Prodigal
[2023-03-19 01:00:55,424] [INFO] Task started: HMMsearch
[2023-03-19 01:00:55,424] [INFO] Running command: hmmsearch --tblout OceanDNA-b36678/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc31185ba-4432-4ade-805a-13fa1632801c/dqc_reference/reference_markers.hmm OceanDNA-b36678/protein.faa > /dev/null
[2023-03-19 01:00:55,639] [INFO] Task succeeded: HMMsearch
[2023-03-19 01:00:55,639] [INFO] Found 6/6 markers.
[2023-03-19 01:00:55,663] [INFO] Query marker FASTA was written to OceanDNA-b36678/markers.fasta
[2023-03-19 01:00:55,664] [INFO] Task started: Blastn
[2023-03-19 01:00:55,664] [INFO] Running command: blastn -query OceanDNA-b36678/markers.fasta -db /var/lib/cwl/stgc31185ba-4432-4ade-805a-13fa1632801c/dqc_reference/reference_markers.fasta -out OceanDNA-b36678/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 01:00:56,363] [INFO] Task succeeded: Blastn
[2023-03-19 01:00:56,364] [INFO] Selected 29 target genomes.
[2023-03-19 01:00:56,364] [INFO] Target genome list was writen to OceanDNA-b36678/target_genomes.txt
[2023-03-19 01:00:56,383] [INFO] Task started: fastANI
[2023-03-19 01:00:56,383] [INFO] Running command: fastANI --query /var/lib/cwl/stg8b29f4f1-0e63-4385-ab63-a9c8932d23f5/OceanDNA-b36678.fa --refList OceanDNA-b36678/target_genomes.txt --output OceanDNA-b36678/fastani_result.tsv --threads 1
[2023-03-19 01:01:14,185] [INFO] Task succeeded: fastANI
[2023-03-19 01:01:14,185] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc31185ba-4432-4ade-805a-13fa1632801c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 01:01:14,186] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc31185ba-4432-4ade-805a-13fa1632801c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 01:01:14,236] [INFO] Found 2 fastANI hits (0 hits with ANI > threshold)
[2023-03-19 01:01:14,236] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-19 01:01:14,236] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Marinagarivorans algicola	strain=Z1	GCA_001292705.1	1513270	1513270	type	True	78.2319	127	837	95	below_threshold
Saccharophagus degradans	strain=2-40	GCA_000013665.1	86304	86304	type	True	76.4314	61	837	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-19 01:01:14,238] [INFO] DFAST Taxonomy check result was written to OceanDNA-b36678/tc_result.tsv
[2023-03-19 01:01:14,239] [INFO] ===== Taxonomy check completed =====
[2023-03-19 01:01:14,239] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 01:01:14,239] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc31185ba-4432-4ade-805a-13fa1632801c/dqc_reference/checkm_data
[2023-03-19 01:01:14,240] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 01:01:14,246] [INFO] Task started: CheckM
[2023-03-19 01:01:14,247] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b36678/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b36678/checkm_input OceanDNA-b36678/checkm_result
[2023-03-19 01:01:55,343] [INFO] Task succeeded: CheckM
[2023-03-19 01:01:55,343] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 84.74%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-19 01:01:55,358] [INFO] ===== Completeness check finished =====
[2023-03-19 01:01:55,358] [INFO] ===== Start GTDB Search =====
[2023-03-19 01:01:55,358] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b36678/markers.fasta)
[2023-03-19 01:01:55,358] [INFO] Task started: Blastn
[2023-03-19 01:01:55,359] [INFO] Running command: blastn -query OceanDNA-b36678/markers.fasta -db /var/lib/cwl/stgc31185ba-4432-4ade-805a-13fa1632801c/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b36678/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 01:01:56,378] [INFO] Task succeeded: Blastn
[2023-03-19 01:01:56,381] [INFO] Selected 29 target genomes.
[2023-03-19 01:01:56,381] [INFO] Target genome list was writen to OceanDNA-b36678/target_genomes_gtdb.txt
[2023-03-19 01:01:56,417] [INFO] Task started: fastANI
[2023-03-19 01:01:56,417] [INFO] Running command: fastANI --query /var/lib/cwl/stg8b29f4f1-0e63-4385-ab63-a9c8932d23f5/OceanDNA-b36678.fa --refList OceanDNA-b36678/target_genomes_gtdb.txt --output OceanDNA-b36678/fastani_result_gtdb.tsv --threads 1
[2023-03-19 01:02:13,214] [INFO] Task succeeded: fastANI
[2023-03-19 01:02:13,216] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-19 01:02:13,216] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001292705.1	s__Marinagarivorans algicola	78.2319	127	837	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Marinagarivorans	95.0	99.28	99.28	0.94	0.94	2	-
GCF_000013665.1	s__Saccharophagus degradans	76.4314	61	837	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Saccharophagus	95.0	96.22	96.22	0.89	0.89	2	-
--------------------------------------------------------------------------------
[2023-03-19 01:02:13,218] [INFO] GTDB search result was written to OceanDNA-b36678/result_gtdb.tsv
[2023-03-19 01:02:13,218] [INFO] ===== GTDB Search completed =====
[2023-03-19 01:02:13,219] [INFO] DFAST_QC result json was written to OceanDNA-b36678/dqc_result.json
[2023-03-19 01:02:13,219] [INFO] DFAST_QC completed!
[2023-03-19 01:02:13,219] [INFO] Total running time: 0h1m34s