[2023-03-15 14:06:11,260] [INFO] DFAST_QC pipeline started.
[2023-03-15 14:06:11,260] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 14:06:11,260] [INFO] DQC Reference Directory: /var/lib/cwl/stg39ec0c7d-f62c-4af1-9038-60559e3a3d2e/dqc_reference
[2023-03-15 14:06:12,351] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 14:06:12,351] [INFO] Task started: Prodigal
[2023-03-15 14:06:12,352] [INFO] Running command: cat /var/lib/cwl/stg254d61ae-59e7-4d53-83e0-5eaf5cff0aac/OceanDNA-b36726.fa | prodigal -d OceanDNA-b36726/cds.fna -a OceanDNA-b36726/protein.faa -g 11 -q > /dev/null
[2023-03-15 14:06:22,314] [INFO] Task succeeded: Prodigal
[2023-03-15 14:06:22,314] [INFO] Task started: HMMsearch
[2023-03-15 14:06:22,314] [INFO] Running command: hmmsearch --tblout OceanDNA-b36726/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg39ec0c7d-f62c-4af1-9038-60559e3a3d2e/dqc_reference/reference_markers.hmm OceanDNA-b36726/protein.faa > /dev/null
[2023-03-15 14:06:22,489] [INFO] Task succeeded: HMMsearch
[2023-03-15 14:06:22,490] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg254d61ae-59e7-4d53-83e0-5eaf5cff0aac/OceanDNA-b36726.fa]
[2023-03-15 14:06:22,508] [INFO] Query marker FASTA was written to OceanDNA-b36726/markers.fasta
[2023-03-15 14:06:22,510] [INFO] Task started: Blastn
[2023-03-15 14:06:22,510] [INFO] Running command: blastn -query OceanDNA-b36726/markers.fasta -db /var/lib/cwl/stg39ec0c7d-f62c-4af1-9038-60559e3a3d2e/dqc_reference/reference_markers.fasta -out OceanDNA-b36726/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 14:06:23,043] [INFO] Task succeeded: Blastn
[2023-03-15 14:06:23,044] [INFO] Selected 19 target genomes.
[2023-03-15 14:06:23,044] [INFO] Target genome list was writen to OceanDNA-b36726/target_genomes.txt
[2023-03-15 14:06:23,057] [INFO] Task started: fastANI
[2023-03-15 14:06:23,057] [INFO] Running command: fastANI --query /var/lib/cwl/stg254d61ae-59e7-4d53-83e0-5eaf5cff0aac/OceanDNA-b36726.fa --refList OceanDNA-b36726/target_genomes.txt --output OceanDNA-b36726/fastani_result.tsv --threads 1
[2023-03-15 14:06:34,441] [INFO] Task succeeded: fastANI
[2023-03-15 14:06:34,441] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg39ec0c7d-f62c-4af1-9038-60559e3a3d2e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 14:06:34,442] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg39ec0c7d-f62c-4af1-9038-60559e3a3d2e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 14:06:34,442] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 14:06:34,442] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 14:06:34,442] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 14:06:34,443] [INFO] DFAST Taxonomy check result was written to OceanDNA-b36726/tc_result.tsv
[2023-03-15 14:06:34,443] [INFO] ===== Taxonomy check completed =====
[2023-03-15 14:06:34,443] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 14:06:34,443] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg39ec0c7d-f62c-4af1-9038-60559e3a3d2e/dqc_reference/checkm_data
[2023-03-15 14:06:34,446] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 14:06:34,585] [INFO] Task started: CheckM
[2023-03-15 14:06:34,585] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b36726/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b36726/checkm_input OceanDNA-b36726/checkm_result
[2023-03-15 14:07:04,604] [INFO] Task succeeded: CheckM
[2023-03-15 14:07:04,604] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.33%
Contamintation: 4.17%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-15 14:07:04,607] [INFO] ===== Completeness check finished =====
[2023-03-15 14:07:04,607] [INFO] ===== Start GTDB Search =====
[2023-03-15 14:07:04,607] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b36726/markers.fasta)
[2023-03-15 14:07:04,609] [INFO] Task started: Blastn
[2023-03-15 14:07:04,609] [INFO] Running command: blastn -query OceanDNA-b36726/markers.fasta -db /var/lib/cwl/stg39ec0c7d-f62c-4af1-9038-60559e3a3d2e/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b36726/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 14:07:05,383] [INFO] Task succeeded: Blastn
[2023-03-15 14:07:05,385] [INFO] Selected 24 target genomes.
[2023-03-15 14:07:05,385] [INFO] Target genome list was writen to OceanDNA-b36726/target_genomes_gtdb.txt
[2023-03-15 14:07:05,431] [INFO] Task started: fastANI
[2023-03-15 14:07:05,431] [INFO] Running command: fastANI --query /var/lib/cwl/stg254d61ae-59e7-4d53-83e0-5eaf5cff0aac/OceanDNA-b36726.fa --refList OceanDNA-b36726/target_genomes_gtdb.txt --output OceanDNA-b36726/fastani_result_gtdb.tsv --threads 1
[2023-03-15 14:07:17,923] [INFO] Task succeeded: fastANI
[2023-03-15 14:07:17,925] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 14:07:17,925] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_003452295.1	s__UBA7455 sp003452295	77.329	52	538	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__HTCC2089;g__UBA7455	95.0	99.39	99.39	0.69	0.69	2	-
--------------------------------------------------------------------------------
[2023-03-15 14:07:17,926] [INFO] GTDB search result was written to OceanDNA-b36726/result_gtdb.tsv
[2023-03-15 14:07:17,926] [INFO] ===== GTDB Search completed =====
[2023-03-15 14:07:17,927] [INFO] DFAST_QC result json was written to OceanDNA-b36726/dqc_result.json
[2023-03-15 14:07:17,928] [INFO] DFAST_QC completed!
[2023-03-15 14:07:17,928] [INFO] Total running time: 0h1m7s