[2023-03-14 15:20:00,451] [INFO] DFAST_QC pipeline started.
[2023-03-14 15:20:00,452] [INFO] DFAST_QC version: 0.5.7
[2023-03-14 15:20:00,452] [INFO] DQC Reference Directory: /var/lib/cwl/stgba91b1f5-ba64-4132-86a5-c4bca081587a/dqc_reference
[2023-03-14 15:20:01,580] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-14 15:20:01,584] [INFO] Task started: Prodigal
[2023-03-14 15:20:01,584] [INFO] Running command: cat /var/lib/cwl/stg0ebd2cb4-c8e2-408b-8840-17bfb1f7fb51/OceanDNA-b36913.fa | prodigal -d OceanDNA-b36913/cds.fna -a OceanDNA-b36913/protein.faa -g 11 -q > /dev/null
[2023-03-14 15:20:12,377] [INFO] Task succeeded: Prodigal
[2023-03-14 15:20:12,377] [INFO] Task started: HMMsearch
[2023-03-14 15:20:12,377] [INFO] Running command: hmmsearch --tblout OceanDNA-b36913/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgba91b1f5-ba64-4132-86a5-c4bca081587a/dqc_reference/reference_markers.hmm OceanDNA-b36913/protein.faa > /dev/null
[2023-03-14 15:20:12,540] [INFO] Task succeeded: HMMsearch
[2023-03-14 15:20:12,540] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg0ebd2cb4-c8e2-408b-8840-17bfb1f7fb51/OceanDNA-b36913.fa]
[2023-03-14 15:20:12,560] [INFO] Query marker FASTA was written to OceanDNA-b36913/markers.fasta
[2023-03-14 15:20:12,562] [INFO] Task started: Blastn
[2023-03-14 15:20:12,562] [INFO] Running command: blastn -query OceanDNA-b36913/markers.fasta -db /var/lib/cwl/stgba91b1f5-ba64-4132-86a5-c4bca081587a/dqc_reference/reference_markers.fasta -out OceanDNA-b36913/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 15:20:13,182] [INFO] Task succeeded: Blastn
[2023-03-14 15:20:13,183] [INFO] Selected 26 target genomes.
[2023-03-14 15:20:13,183] [INFO] Target genome list was writen to OceanDNA-b36913/target_genomes.txt
[2023-03-14 15:20:13,199] [INFO] Task started: fastANI
[2023-03-14 15:20:13,199] [INFO] Running command: fastANI --query /var/lib/cwl/stg0ebd2cb4-c8e2-408b-8840-17bfb1f7fb51/OceanDNA-b36913.fa --refList OceanDNA-b36913/target_genomes.txt --output OceanDNA-b36913/fastani_result.tsv --threads 1
[2023-03-14 15:20:34,261] [INFO] Task succeeded: fastANI
[2023-03-14 15:20:34,261] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgba91b1f5-ba64-4132-86a5-c4bca081587a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-14 15:20:34,262] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgba91b1f5-ba64-4132-86a5-c4bca081587a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-14 15:20:34,262] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-14 15:20:34,262] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-14 15:20:34,262] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-14 15:20:34,262] [INFO] DFAST Taxonomy check result was written to OceanDNA-b36913/tc_result.tsv
[2023-03-14 15:20:34,262] [INFO] ===== Taxonomy check completed =====
[2023-03-14 15:20:34,262] [INFO] ===== Start completeness check using CheckM =====
[2023-03-14 15:20:34,262] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgba91b1f5-ba64-4132-86a5-c4bca081587a/dqc_reference/checkm_data
[2023-03-14 15:20:34,335] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-14 15:20:34,345] [INFO] Task started: CheckM
[2023-03-14 15:20:34,345] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b36913/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b36913/checkm_input OceanDNA-b36913/checkm_result
[2023-03-14 15:21:19,778] [INFO] Task succeeded: CheckM
[2023-03-14 15:21:19,778] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 79.17%
Contamintation: 0.38%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-14 15:21:19,781] [INFO] ===== Completeness check finished =====
[2023-03-14 15:21:19,781] [INFO] ===== Start GTDB Search =====
[2023-03-14 15:21:19,781] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b36913/markers.fasta)
[2023-03-14 15:21:19,783] [INFO] Task started: Blastn
[2023-03-14 15:21:19,783] [INFO] Running command: blastn -query OceanDNA-b36913/markers.fasta -db /var/lib/cwl/stgba91b1f5-ba64-4132-86a5-c4bca081587a/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b36913/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-14 15:21:22,397] [INFO] Task succeeded: Blastn
[2023-03-14 15:21:22,398] [INFO] Selected 30 target genomes.
[2023-03-14 15:21:22,399] [INFO] Target genome list was writen to OceanDNA-b36913/target_genomes_gtdb.txt
[2023-03-14 15:21:22,607] [INFO] Task started: fastANI
[2023-03-14 15:21:22,607] [INFO] Running command: fastANI --query /var/lib/cwl/stg0ebd2cb4-c8e2-408b-8840-17bfb1f7fb51/OceanDNA-b36913.fa --refList OceanDNA-b36913/target_genomes_gtdb.txt --output OceanDNA-b36913/fastani_result_gtdb.tsv --threads 1
[2023-03-14 15:21:47,518] [INFO] Task succeeded: fastANI
[2023-03-14 15:21:47,519] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-14 15:21:47,519] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-14 15:21:47,519] [INFO] GTDB search result was written to OceanDNA-b36913/result_gtdb.tsv
[2023-03-14 15:21:47,519] [INFO] ===== GTDB Search completed =====
[2023-03-14 15:21:47,520] [INFO] DFAST_QC result json was written to OceanDNA-b36913/dqc_result.json
[2023-03-14 15:21:47,520] [INFO] DFAST_QC completed!
[2023-03-14 15:21:47,520] [INFO] Total running time: 0h1m47s