[2023-03-16 08:02:40,409] [INFO] DFAST_QC pipeline started.
[2023-03-16 08:02:40,410] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 08:02:40,411] [INFO] DQC Reference Directory: /var/lib/cwl/stg2888e06d-75b5-4335-bc26-3057618a5089/dqc_reference
[2023-03-16 08:02:41,598] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 08:02:41,599] [INFO] Task started: Prodigal
[2023-03-16 08:02:41,599] [INFO] Running command: cat /var/lib/cwl/stgeeb13372-3b72-4b09-82e0-80218feaa2d2/OceanDNA-b37118.fa | prodigal -d OceanDNA-b37118/cds.fna -a OceanDNA-b37118/protein.faa -g 11 -q > /dev/null
[2023-03-16 08:02:50,071] [INFO] Task succeeded: Prodigal
[2023-03-16 08:02:50,071] [INFO] Task started: HMMsearch
[2023-03-16 08:02:50,071] [INFO] Running command: hmmsearch --tblout OceanDNA-b37118/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg2888e06d-75b5-4335-bc26-3057618a5089/dqc_reference/reference_markers.hmm OceanDNA-b37118/protein.faa > /dev/null
[2023-03-16 08:02:50,281] [INFO] Task succeeded: HMMsearch
[2023-03-16 08:02:50,281] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgeeb13372-3b72-4b09-82e0-80218feaa2d2/OceanDNA-b37118.fa]
[2023-03-16 08:02:50,305] [INFO] Query marker FASTA was written to OceanDNA-b37118/markers.fasta
[2023-03-16 08:02:50,305] [INFO] Task started: Blastn
[2023-03-16 08:02:50,305] [INFO] Running command: blastn -query OceanDNA-b37118/markers.fasta -db /var/lib/cwl/stg2888e06d-75b5-4335-bc26-3057618a5089/dqc_reference/reference_markers.fasta -out OceanDNA-b37118/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 08:02:50,875] [INFO] Task succeeded: Blastn
[2023-03-16 08:02:50,880] [INFO] Selected 35 target genomes.
[2023-03-16 08:02:50,880] [INFO] Target genome list was writen to OceanDNA-b37118/target_genomes.txt
[2023-03-16 08:02:51,013] [INFO] Task started: fastANI
[2023-03-16 08:02:51,013] [INFO] Running command: fastANI --query /var/lib/cwl/stgeeb13372-3b72-4b09-82e0-80218feaa2d2/OceanDNA-b37118.fa --refList OceanDNA-b37118/target_genomes.txt --output OceanDNA-b37118/fastani_result.tsv --threads 1
[2023-03-16 08:03:13,591] [INFO] Task succeeded: fastANI
[2023-03-16 08:03:13,592] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg2888e06d-75b5-4335-bc26-3057618a5089/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 08:03:13,592] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg2888e06d-75b5-4335-bc26-3057618a5089/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 08:03:13,592] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 08:03:13,592] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 08:03:13,592] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 08:03:13,609] [INFO] DFAST Taxonomy check result was written to OceanDNA-b37118/tc_result.tsv
[2023-03-16 08:03:13,629] [INFO] ===== Taxonomy check completed =====
[2023-03-16 08:03:13,629] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 08:03:13,629] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg2888e06d-75b5-4335-bc26-3057618a5089/dqc_reference/checkm_data
[2023-03-16 08:03:13,633] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 08:03:13,636] [INFO] Task started: CheckM
[2023-03-16 08:03:13,636] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b37118/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b37118/checkm_input OceanDNA-b37118/checkm_result
[2023-03-16 08:03:39,951] [INFO] Task succeeded: CheckM
[2023-03-16 08:03:39,951] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.33%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 08:03:39,966] [INFO] ===== Completeness check finished =====
[2023-03-16 08:03:39,967] [INFO] ===== Start GTDB Search =====
[2023-03-16 08:03:39,967] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b37118/markers.fasta)
[2023-03-16 08:03:39,968] [INFO] Task started: Blastn
[2023-03-16 08:03:39,968] [INFO] Running command: blastn -query OceanDNA-b37118/markers.fasta -db /var/lib/cwl/stg2888e06d-75b5-4335-bc26-3057618a5089/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b37118/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 08:03:40,845] [INFO] Task succeeded: Blastn
[2023-03-16 08:03:40,846] [INFO] Selected 13 target genomes.
[2023-03-16 08:03:40,847] [INFO] Target genome list was writen to OceanDNA-b37118/target_genomes_gtdb.txt
[2023-03-16 08:03:41,266] [INFO] Task started: fastANI
[2023-03-16 08:03:41,267] [INFO] Running command: fastANI --query /var/lib/cwl/stgeeb13372-3b72-4b09-82e0-80218feaa2d2/OceanDNA-b37118.fa --refList OceanDNA-b37118/target_genomes_gtdb.txt --output OceanDNA-b37118/fastani_result_gtdb.tsv --threads 1
[2023-03-16 08:03:47,841] [INFO] Task succeeded: fastANI
[2023-03-16 08:03:47,845] [INFO] Found 5 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-16 08:03:47,845] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002684495.1	s__UBA4421 sp002684495	99.7872	364	369	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__HTCC2089;g__UBA4421	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002690405.1	s__UBA4421 sp002690405	89.9894	350	369	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__HTCC2089;g__UBA4421	95.0	98.88	98.84	0.89	0.88	4	-
GCA_002704325.1	s__UBA4421 sp002704325	80.3526	182	369	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__HTCC2089;g__UBA4421	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002725095.1	s__UBA4421 sp002725095	78.1898	134	369	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__HTCC2089;g__UBA4421	95.0	99.07	99.07	0.73	0.73	2	-
GCA_002348825.1	s__UBA4421 sp002348825	77.3848	147	369	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__HTCC2089;g__UBA4421	95.0	99.36	99.22	0.85	0.84	3	-
--------------------------------------------------------------------------------
[2023-03-16 08:03:47,845] [INFO] GTDB search result was written to OceanDNA-b37118/result_gtdb.tsv
[2023-03-16 08:03:47,845] [INFO] ===== GTDB Search completed =====
[2023-03-16 08:03:47,846] [INFO] DFAST_QC result json was written to OceanDNA-b37118/dqc_result.json
[2023-03-16 08:03:47,846] [INFO] DFAST_QC completed!
[2023-03-16 08:03:47,846] [INFO] Total running time: 0h1m7s