[2023-03-15 11:22:20,473] [INFO] DFAST_QC pipeline started.
[2023-03-15 11:22:20,473] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 11:22:20,473] [INFO] DQC Reference Directory: /var/lib/cwl/stg88f405ef-291d-487a-a395-3d57fc329bea/dqc_reference
[2023-03-15 11:22:21,948] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 11:22:21,949] [INFO] Task started: Prodigal
[2023-03-15 11:22:21,949] [INFO] Running command: cat /var/lib/cwl/stgb4f515b0-3137-4145-bc42-c5773d8316b9/OceanDNA-b37250.fa | prodigal -d OceanDNA-b37250/cds.fna -a OceanDNA-b37250/protein.faa -g 11 -q > /dev/null
[2023-03-15 11:22:33,850] [INFO] Task succeeded: Prodigal
[2023-03-15 11:22:33,850] [INFO] Task started: HMMsearch
[2023-03-15 11:22:33,850] [INFO] Running command: hmmsearch --tblout OceanDNA-b37250/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg88f405ef-291d-487a-a395-3d57fc329bea/dqc_reference/reference_markers.hmm OceanDNA-b37250/protein.faa > /dev/null
[2023-03-15 11:22:34,034] [INFO] Task succeeded: HMMsearch
[2023-03-15 11:22:34,034] [INFO] Found 6/6 markers.
[2023-03-15 11:22:34,048] [INFO] Query marker FASTA was written to OceanDNA-b37250/markers.fasta
[2023-03-15 11:22:34,048] [INFO] Task started: Blastn
[2023-03-15 11:22:34,048] [INFO] Running command: blastn -query OceanDNA-b37250/markers.fasta -db /var/lib/cwl/stg88f405ef-291d-487a-a395-3d57fc329bea/dqc_reference/reference_markers.fasta -out OceanDNA-b37250/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 11:22:34,605] [INFO] Task succeeded: Blastn
[2023-03-15 11:22:34,606] [INFO] Selected 17 target genomes.
[2023-03-15 11:22:34,606] [INFO] Target genome list was writen to OceanDNA-b37250/target_genomes.txt
[2023-03-15 11:22:34,618] [INFO] Task started: fastANI
[2023-03-15 11:22:34,619] [INFO] Running command: fastANI --query /var/lib/cwl/stgb4f515b0-3137-4145-bc42-c5773d8316b9/OceanDNA-b37250.fa --refList OceanDNA-b37250/target_genomes.txt --output OceanDNA-b37250/fastani_result.tsv --threads 1
[2023-03-15 11:22:42,975] [INFO] Task succeeded: fastANI
[2023-03-15 11:22:42,975] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg88f405ef-291d-487a-a395-3d57fc329bea/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 11:22:42,975] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg88f405ef-291d-487a-a395-3d57fc329bea/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 11:22:42,975] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 11:22:42,975] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 11:22:42,976] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 11:22:42,976] [INFO] DFAST Taxonomy check result was written to OceanDNA-b37250/tc_result.tsv
[2023-03-15 11:22:42,976] [INFO] ===== Taxonomy check completed =====
[2023-03-15 11:22:42,976] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 11:22:42,976] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg88f405ef-291d-487a-a395-3d57fc329bea/dqc_reference/checkm_data
[2023-03-15 11:22:42,978] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 11:22:42,982] [INFO] Task started: CheckM
[2023-03-15 11:22:42,982] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b37250/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b37250/checkm_input OceanDNA-b37250/checkm_result
[2023-03-15 11:23:16,233] [INFO] Task succeeded: CheckM
[2023-03-15 11:23:16,234] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 11:23:16,236] [INFO] ===== Completeness check finished =====
[2023-03-15 11:23:16,236] [INFO] ===== Start GTDB Search =====
[2023-03-15 11:23:16,236] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b37250/markers.fasta)
[2023-03-15 11:23:16,237] [INFO] Task started: Blastn
[2023-03-15 11:23:16,237] [INFO] Running command: blastn -query OceanDNA-b37250/markers.fasta -db /var/lib/cwl/stg88f405ef-291d-487a-a395-3d57fc329bea/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b37250/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 11:23:17,109] [INFO] Task succeeded: Blastn
[2023-03-15 11:23:17,110] [INFO] Selected 20 target genomes.
[2023-03-15 11:23:17,110] [INFO] Target genome list was writen to OceanDNA-b37250/target_genomes_gtdb.txt
[2023-03-15 11:23:17,142] [INFO] Task started: fastANI
[2023-03-15 11:23:17,142] [INFO] Running command: fastANI --query /var/lib/cwl/stgb4f515b0-3137-4145-bc42-c5773d8316b9/OceanDNA-b37250.fa --refList OceanDNA-b37250/target_genomes_gtdb.txt --output OceanDNA-b37250/fastani_result_gtdb.tsv --threads 1
[2023-03-15 11:23:28,812] [INFO] Task succeeded: fastANI
[2023-03-15 11:23:28,815] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 11:23:28,815] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002167855.2	s__UBA7455 sp002167855	99.7563	641	724	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__HTCC2089;g__UBA7455	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002690865.1	s__UBA7455 sp002690865	79.1957	352	724	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__HTCC2089;g__UBA7455	95.0	99.94	99.88	0.94	0.91	3	-
--------------------------------------------------------------------------------
[2023-03-15 11:23:28,815] [INFO] GTDB search result was written to OceanDNA-b37250/result_gtdb.tsv
[2023-03-15 11:23:28,815] [INFO] ===== GTDB Search completed =====
[2023-03-15 11:23:28,815] [INFO] DFAST_QC result json was written to OceanDNA-b37250/dqc_result.json
[2023-03-15 11:23:28,816] [INFO] DFAST_QC completed!
[2023-03-15 11:23:28,816] [INFO] Total running time: 0h1m8s