[2023-03-18 22:12:23,603] [INFO] DFAST_QC pipeline started.
[2023-03-18 22:12:23,603] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 22:12:23,603] [INFO] DQC Reference Directory: /var/lib/cwl/stga2d09b49-bfc8-4fbd-ab79-2347fc5e613e/dqc_reference
[2023-03-18 22:12:24,721] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 22:12:24,722] [INFO] Task started: Prodigal
[2023-03-18 22:12:24,722] [INFO] Running command: cat /var/lib/cwl/stgaca8f650-96f5-4d7b-ac2d-ad4f02143035/OceanDNA-b37354.fa | prodigal -d OceanDNA-b37354/cds.fna -a OceanDNA-b37354/protein.faa -g 11 -q > /dev/null
[2023-03-18 22:12:35,088] [INFO] Task succeeded: Prodigal
[2023-03-18 22:12:35,088] [INFO] Task started: HMMsearch
[2023-03-18 22:12:35,088] [INFO] Running command: hmmsearch --tblout OceanDNA-b37354/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga2d09b49-bfc8-4fbd-ab79-2347fc5e613e/dqc_reference/reference_markers.hmm OceanDNA-b37354/protein.faa > /dev/null
[2023-03-18 22:12:35,292] [INFO] Task succeeded: HMMsearch
[2023-03-18 22:12:35,293] [INFO] Found 6/6 markers.
[2023-03-18 22:12:35,312] [INFO] Query marker FASTA was written to OceanDNA-b37354/markers.fasta
[2023-03-18 22:12:35,313] [INFO] Task started: Blastn
[2023-03-18 22:12:35,313] [INFO] Running command: blastn -query OceanDNA-b37354/markers.fasta -db /var/lib/cwl/stga2d09b49-bfc8-4fbd-ab79-2347fc5e613e/dqc_reference/reference_markers.fasta -out OceanDNA-b37354/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 22:12:35,961] [INFO] Task succeeded: Blastn
[2023-03-18 22:12:35,962] [INFO] Selected 39 target genomes.
[2023-03-18 22:12:35,962] [INFO] Target genome list was writen to OceanDNA-b37354/target_genomes.txt
[2023-03-18 22:12:35,982] [INFO] Task started: fastANI
[2023-03-18 22:12:35,982] [INFO] Running command: fastANI --query /var/lib/cwl/stgaca8f650-96f5-4d7b-ac2d-ad4f02143035/OceanDNA-b37354.fa --refList OceanDNA-b37354/target_genomes.txt --output OceanDNA-b37354/fastani_result.tsv --threads 1
[2023-03-18 22:13:02,142] [INFO] Task succeeded: fastANI
[2023-03-18 22:13:02,143] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga2d09b49-bfc8-4fbd-ab79-2347fc5e613e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 22:13:02,143] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga2d09b49-bfc8-4fbd-ab79-2347fc5e613e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 22:13:02,143] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 22:13:02,143] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 22:13:02,143] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 22:13:02,144] [INFO] DFAST Taxonomy check result was written to OceanDNA-b37354/tc_result.tsv
[2023-03-18 22:13:02,146] [INFO] ===== Taxonomy check completed =====
[2023-03-18 22:13:02,147] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 22:13:02,147] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga2d09b49-bfc8-4fbd-ab79-2347fc5e613e/dqc_reference/checkm_data
[2023-03-18 22:13:02,149] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 22:13:02,155] [INFO] Task started: CheckM
[2023-03-18 22:13:02,155] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b37354/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b37354/checkm_input OceanDNA-b37354/checkm_result
[2023-03-18 22:13:33,085] [INFO] Task succeeded: CheckM
[2023-03-18 22:13:33,085] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 22:13:33,088] [INFO] ===== Completeness check finished =====
[2023-03-18 22:13:33,088] [INFO] ===== Start GTDB Search =====
[2023-03-18 22:13:33,088] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b37354/markers.fasta)
[2023-03-18 22:13:33,089] [INFO] Task started: Blastn
[2023-03-18 22:13:33,089] [INFO] Running command: blastn -query OceanDNA-b37354/markers.fasta -db /var/lib/cwl/stga2d09b49-bfc8-4fbd-ab79-2347fc5e613e/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b37354/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 22:13:34,220] [INFO] Task succeeded: Blastn
[2023-03-18 22:13:34,221] [INFO] Selected 35 target genomes.
[2023-03-18 22:13:34,221] [INFO] Target genome list was writen to OceanDNA-b37354/target_genomes_gtdb.txt
[2023-03-18 22:13:34,252] [INFO] Task started: fastANI
[2023-03-18 22:13:34,252] [INFO] Running command: fastANI --query /var/lib/cwl/stgaca8f650-96f5-4d7b-ac2d-ad4f02143035/OceanDNA-b37354.fa --refList OceanDNA-b37354/target_genomes_gtdb.txt --output OceanDNA-b37354/fastani_result_gtdb.tsv --threads 1
[2023-03-18 22:13:56,804] [INFO] Task succeeded: fastANI
[2023-03-18 22:13:56,806] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-18 22:13:56,806] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018656865.1	s__UBA9659 sp018656865	99.814	488	523	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__HTCC2089;g__UBA9659	95.0	99.73	99.68	0.90	0.90	3	conclusive
--------------------------------------------------------------------------------
[2023-03-18 22:13:56,807] [INFO] GTDB search result was written to OceanDNA-b37354/result_gtdb.tsv
[2023-03-18 22:13:56,807] [INFO] ===== GTDB Search completed =====
[2023-03-18 22:13:56,807] [INFO] DFAST_QC result json was written to OceanDNA-b37354/dqc_result.json
[2023-03-18 22:13:56,807] [INFO] DFAST_QC completed!
[2023-03-18 22:13:56,807] [INFO] Total running time: 0h1m33s