[2023-03-16 17:36:12,601] [INFO] DFAST_QC pipeline started.
[2023-03-16 17:36:12,601] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 17:36:12,602] [INFO] DQC Reference Directory: /var/lib/cwl/stge3e0df27-119a-4daf-88c0-9c94e474585d/dqc_reference
[2023-03-16 17:36:13,754] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 17:36:13,755] [INFO] Task started: Prodigal
[2023-03-16 17:36:13,756] [INFO] Running command: cat /var/lib/cwl/stg4cdd08fd-c121-4e97-a10a-4181cdc752f1/OceanDNA-b37733.fa | prodigal -d OceanDNA-b37733/cds.fna -a OceanDNA-b37733/protein.faa -g 11 -q > /dev/null
[2023-03-16 17:36:27,327] [INFO] Task succeeded: Prodigal
[2023-03-16 17:36:27,328] [INFO] Task started: HMMsearch
[2023-03-16 17:36:27,328] [INFO] Running command: hmmsearch --tblout OceanDNA-b37733/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge3e0df27-119a-4daf-88c0-9c94e474585d/dqc_reference/reference_markers.hmm OceanDNA-b37733/protein.faa > /dev/null
[2023-03-16 17:36:27,516] [INFO] Task succeeded: HMMsearch
[2023-03-16 17:36:27,516] [INFO] Found 6/6 markers.
[2023-03-16 17:36:27,535] [INFO] Query marker FASTA was written to OceanDNA-b37733/markers.fasta
[2023-03-16 17:36:27,537] [INFO] Task started: Blastn
[2023-03-16 17:36:27,537] [INFO] Running command: blastn -query OceanDNA-b37733/markers.fasta -db /var/lib/cwl/stge3e0df27-119a-4daf-88c0-9c94e474585d/dqc_reference/reference_markers.fasta -out OceanDNA-b37733/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 17:36:28,110] [INFO] Task succeeded: Blastn
[2023-03-16 17:36:28,111] [INFO] Selected 39 target genomes.
[2023-03-16 17:36:28,111] [INFO] Target genome list was writen to OceanDNA-b37733/target_genomes.txt
[2023-03-16 17:36:28,234] [INFO] Task started: fastANI
[2023-03-16 17:36:28,234] [INFO] Running command: fastANI --query /var/lib/cwl/stg4cdd08fd-c121-4e97-a10a-4181cdc752f1/OceanDNA-b37733.fa --refList OceanDNA-b37733/target_genomes.txt --output OceanDNA-b37733/fastani_result.tsv --threads 1
[2023-03-16 17:36:53,778] [INFO] Task succeeded: fastANI
[2023-03-16 17:36:53,779] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stge3e0df27-119a-4daf-88c0-9c94e474585d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 17:36:53,779] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stge3e0df27-119a-4daf-88c0-9c94e474585d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 17:36:53,779] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 17:36:53,779] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 17:36:53,779] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 17:36:53,779] [INFO] DFAST Taxonomy check result was written to OceanDNA-b37733/tc_result.tsv
[2023-03-16 17:36:53,780] [INFO] ===== Taxonomy check completed =====
[2023-03-16 17:36:53,780] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 17:36:53,780] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge3e0df27-119a-4daf-88c0-9c94e474585d/dqc_reference/checkm_data
[2023-03-16 17:36:53,783] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 17:36:53,837] [INFO] Task started: CheckM
[2023-03-16 17:36:53,838] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b37733/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b37733/checkm_input OceanDNA-b37733/checkm_result
[2023-03-16 17:37:38,353] [INFO] Task succeeded: CheckM
[2023-03-16 17:37:38,354] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 90.77%
Contamintation: 9.38%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-16 17:37:38,356] [INFO] ===== Completeness check finished =====
[2023-03-16 17:37:38,356] [INFO] ===== Start GTDB Search =====
[2023-03-16 17:37:38,356] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b37733/markers.fasta)
[2023-03-16 17:37:38,365] [INFO] Task started: Blastn
[2023-03-16 17:37:38,366] [INFO] Running command: blastn -query OceanDNA-b37733/markers.fasta -db /var/lib/cwl/stge3e0df27-119a-4daf-88c0-9c94e474585d/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b37733/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 17:37:40,211] [INFO] Task succeeded: Blastn
[2023-03-16 17:37:40,211] [INFO] Selected 15 target genomes.
[2023-03-16 17:37:40,212] [INFO] Target genome list was writen to OceanDNA-b37733/target_genomes_gtdb.txt
[2023-03-16 17:37:40,225] [INFO] Task started: fastANI
[2023-03-16 17:37:40,225] [INFO] Running command: fastANI --query /var/lib/cwl/stg4cdd08fd-c121-4e97-a10a-4181cdc752f1/OceanDNA-b37733.fa --refList OceanDNA-b37733/target_genomes_gtdb.txt --output OceanDNA-b37733/fastani_result_gtdb.tsv --threads 1
[2023-03-16 17:37:51,550] [INFO] Task succeeded: fastANI
[2023-03-16 17:37:51,557] [INFO] Found 10 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 17:37:51,557] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018222695.1	s__Luminiphilus sp018222695	94.5551	381	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016778085.1	s__Luminiphilus sp016778085	93.5525	366	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003331685.1	s__Luminiphilus sp003331685	89.9635	287	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902518075.1	s__Luminiphilus sp902518075	84.911	250	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902520195.1	s__Luminiphilus sp902520195	84.7289	359	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	96.03	95.89	0.61	0.58	3	-
GCA_902623175.1	s__Luminiphilus sp902623175	84.1744	440	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013911345.1	s__Luminiphilus sp013911345	83.5097	199	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016778045.1	s__Luminiphilus sp016778045	80.8466	231	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002721785.1	s__Luminiphilus sp002721785	78.8569	223	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	99.68	99.68	0.89	0.89	2	-
GCA_008081075.1	s__Luminiphilus sp008081075	78.0186	96	731	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 17:37:51,557] [INFO] GTDB search result was written to OceanDNA-b37733/result_gtdb.tsv
[2023-03-16 17:37:51,557] [INFO] ===== GTDB Search completed =====
[2023-03-16 17:37:51,558] [INFO] DFAST_QC result json was written to OceanDNA-b37733/dqc_result.json
[2023-03-16 17:37:51,558] [INFO] DFAST_QC completed!
[2023-03-16 17:37:51,558] [INFO] Total running time: 0h1m39s