[2023-03-18 23:56:04,493] [INFO] DFAST_QC pipeline started.
[2023-03-18 23:56:04,493] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 23:56:04,494] [INFO] DQC Reference Directory: /var/lib/cwl/stg0dd8dfd5-2c34-4b9a-a50e-68eca4841fcd/dqc_reference
[2023-03-18 23:56:05,620] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 23:56:05,621] [INFO] Task started: Prodigal
[2023-03-18 23:56:05,621] [INFO] Running command: cat /var/lib/cwl/stgd768ae7d-9e66-4838-acde-b75b5bb2311d/OceanDNA-b37771.fa | prodigal -d OceanDNA-b37771/cds.fna -a OceanDNA-b37771/protein.faa -g 11 -q > /dev/null
[2023-03-18 23:56:18,792] [INFO] Task succeeded: Prodigal
[2023-03-18 23:56:18,793] [INFO] Task started: HMMsearch
[2023-03-18 23:56:18,793] [INFO] Running command: hmmsearch --tblout OceanDNA-b37771/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg0dd8dfd5-2c34-4b9a-a50e-68eca4841fcd/dqc_reference/reference_markers.hmm OceanDNA-b37771/protein.faa > /dev/null
[2023-03-18 23:56:18,975] [INFO] Task succeeded: HMMsearch
[2023-03-18 23:56:18,975] [INFO] Found 6/6 markers.
[2023-03-18 23:56:18,992] [INFO] Query marker FASTA was written to OceanDNA-b37771/markers.fasta
[2023-03-18 23:56:18,992] [INFO] Task started: Blastn
[2023-03-18 23:56:18,992] [INFO] Running command: blastn -query OceanDNA-b37771/markers.fasta -db /var/lib/cwl/stg0dd8dfd5-2c34-4b9a-a50e-68eca4841fcd/dqc_reference/reference_markers.fasta -out OceanDNA-b37771/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 23:56:19,595] [INFO] Task succeeded: Blastn
[2023-03-18 23:56:19,597] [INFO] Selected 34 target genomes.
[2023-03-18 23:56:19,597] [INFO] Target genome list was writen to OceanDNA-b37771/target_genomes.txt
[2023-03-18 23:56:19,638] [INFO] Task started: fastANI
[2023-03-18 23:56:19,638] [INFO] Running command: fastANI --query /var/lib/cwl/stgd768ae7d-9e66-4838-acde-b75b5bb2311d/OceanDNA-b37771.fa --refList OceanDNA-b37771/target_genomes.txt --output OceanDNA-b37771/fastani_result.tsv --threads 1
[2023-03-18 23:56:41,463] [INFO] Task succeeded: fastANI
[2023-03-18 23:56:41,463] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg0dd8dfd5-2c34-4b9a-a50e-68eca4841fcd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 23:56:41,463] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg0dd8dfd5-2c34-4b9a-a50e-68eca4841fcd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 23:56:41,464] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 23:56:41,464] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 23:56:41,464] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 23:56:41,464] [INFO] DFAST Taxonomy check result was written to OceanDNA-b37771/tc_result.tsv
[2023-03-18 23:56:41,464] [INFO] ===== Taxonomy check completed =====
[2023-03-18 23:56:41,464] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 23:56:41,464] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg0dd8dfd5-2c34-4b9a-a50e-68eca4841fcd/dqc_reference/checkm_data
[2023-03-18 23:56:41,467] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 23:56:41,471] [INFO] Task started: CheckM
[2023-03-18 23:56:41,471] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b37771/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b37771/checkm_input OceanDNA-b37771/checkm_result
[2023-03-18 23:57:18,011] [INFO] Task succeeded: CheckM
[2023-03-18 23:57:18,011] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 23:57:18,039] [INFO] ===== Completeness check finished =====
[2023-03-18 23:57:18,039] [INFO] ===== Start GTDB Search =====
[2023-03-18 23:57:18,039] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b37771/markers.fasta)
[2023-03-18 23:57:18,040] [INFO] Task started: Blastn
[2023-03-18 23:57:18,040] [INFO] Running command: blastn -query OceanDNA-b37771/markers.fasta -db /var/lib/cwl/stg0dd8dfd5-2c34-4b9a-a50e-68eca4841fcd/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b37771/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 23:57:19,052] [INFO] Task succeeded: Blastn
[2023-03-18 23:57:19,053] [INFO] Selected 21 target genomes.
[2023-03-18 23:57:19,054] [INFO] Target genome list was writen to OceanDNA-b37771/target_genomes_gtdb.txt
[2023-03-18 23:57:19,072] [INFO] Task started: fastANI
[2023-03-18 23:57:19,072] [INFO] Running command: fastANI --query /var/lib/cwl/stgd768ae7d-9e66-4838-acde-b75b5bb2311d/OceanDNA-b37771.fa --refList OceanDNA-b37771/target_genomes_gtdb.txt --output OceanDNA-b37771/fastani_result_gtdb.tsv --threads 1
[2023-03-18 23:57:29,577] [INFO] Task succeeded: fastANI
[2023-03-18 23:57:29,582] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-18 23:57:29,582] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_905478845.1	s__Luminiphilus sp905478845	99.082	581	714	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_905182485.1	s__Luminiphilus sp905182485	86.3673	459	714	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018666055.1	s__Luminiphilus sp018666055	83.649	293	714	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002683335.1	s__Luminiphilus sp002683335	77.5661	61	714	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	98.43	98.43	0.60	0.60	2	-
GCA_002683395.1	s__Luminiphilus sp002683395	77.5172	70	714	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	98.62	98.62	0.87	0.87	2	-
GCA_002721785.1	s__Luminiphilus sp002721785	76.2423	78	714	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	99.68	99.68	0.89	0.89	2	-
GCA_902623175.1	s__Luminiphilus sp902623175	76.1917	58	714	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 23:57:29,582] [INFO] GTDB search result was written to OceanDNA-b37771/result_gtdb.tsv
[2023-03-18 23:57:29,582] [INFO] ===== GTDB Search completed =====
[2023-03-18 23:57:29,583] [INFO] DFAST_QC result json was written to OceanDNA-b37771/dqc_result.json
[2023-03-18 23:57:29,584] [INFO] DFAST_QC completed!
[2023-03-18 23:57:29,584] [INFO] Total running time: 0h1m25s