[2023-03-17 11:36:53,885] [INFO] DFAST_QC pipeline started.
[2023-03-17 11:36:53,885] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 11:36:53,885] [INFO] DQC Reference Directory: /var/lib/cwl/stg1df3235b-32f6-45e5-9f09-7ac9dea73c4c/dqc_reference
[2023-03-17 11:36:55,055] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 11:36:55,055] [INFO] Task started: Prodigal
[2023-03-17 11:36:55,055] [INFO] Running command: cat /var/lib/cwl/stg9321edaf-c8b2-4915-aa5f-143c53448199/OceanDNA-b37857.fa | prodigal -d OceanDNA-b37857/cds.fna -a OceanDNA-b37857/protein.faa -g 11 -q > /dev/null
[2023-03-17 11:37:00,850] [INFO] Task succeeded: Prodigal
[2023-03-17 11:37:00,850] [INFO] Task started: HMMsearch
[2023-03-17 11:37:00,850] [INFO] Running command: hmmsearch --tblout OceanDNA-b37857/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1df3235b-32f6-45e5-9f09-7ac9dea73c4c/dqc_reference/reference_markers.hmm OceanDNA-b37857/protein.faa > /dev/null
[2023-03-17 11:37:00,992] [INFO] Task succeeded: HMMsearch
[2023-03-17 11:37:00,992] [INFO] Found 6/6 markers.
[2023-03-17 11:37:01,014] [INFO] Query marker FASTA was written to OceanDNA-b37857/markers.fasta
[2023-03-17 11:37:01,014] [INFO] Task started: Blastn
[2023-03-17 11:37:01,014] [INFO] Running command: blastn -query OceanDNA-b37857/markers.fasta -db /var/lib/cwl/stg1df3235b-32f6-45e5-9f09-7ac9dea73c4c/dqc_reference/reference_markers.fasta -out OceanDNA-b37857/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 11:37:01,560] [INFO] Task succeeded: Blastn
[2023-03-17 11:37:01,564] [INFO] Selected 34 target genomes.
[2023-03-17 11:37:01,564] [INFO] Target genome list was writen to OceanDNA-b37857/target_genomes.txt
[2023-03-17 11:37:01,587] [INFO] Task started: fastANI
[2023-03-17 11:37:01,587] [INFO] Running command: fastANI --query /var/lib/cwl/stg9321edaf-c8b2-4915-aa5f-143c53448199/OceanDNA-b37857.fa --refList OceanDNA-b37857/target_genomes.txt --output OceanDNA-b37857/fastani_result.tsv --threads 1
[2023-03-17 11:37:22,433] [INFO] Task succeeded: fastANI
[2023-03-17 11:37:22,433] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1df3235b-32f6-45e5-9f09-7ac9dea73c4c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 11:37:22,433] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1df3235b-32f6-45e5-9f09-7ac9dea73c4c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 11:37:22,434] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 11:37:22,434] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 11:37:22,434] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 11:37:22,435] [INFO] DFAST Taxonomy check result was written to OceanDNA-b37857/tc_result.tsv
[2023-03-17 11:37:22,435] [INFO] ===== Taxonomy check completed =====
[2023-03-17 11:37:22,435] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 11:37:22,435] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1df3235b-32f6-45e5-9f09-7ac9dea73c4c/dqc_reference/checkm_data
[2023-03-17 11:37:22,438] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 11:37:22,794] [INFO] Task started: CheckM
[2023-03-17 11:37:22,794] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b37857/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b37857/checkm_input OceanDNA-b37857/checkm_result
[2023-03-17 11:37:43,715] [INFO] Task succeeded: CheckM
[2023-03-17 11:37:43,715] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 74.48%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-17 11:37:43,718] [INFO] ===== Completeness check finished =====
[2023-03-17 11:37:43,718] [INFO] ===== Start GTDB Search =====
[2023-03-17 11:37:43,718] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b37857/markers.fasta)
[2023-03-17 11:37:43,719] [INFO] Task started: Blastn
[2023-03-17 11:37:43,719] [INFO] Running command: blastn -query OceanDNA-b37857/markers.fasta -db /var/lib/cwl/stg1df3235b-32f6-45e5-9f09-7ac9dea73c4c/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b37857/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 11:37:44,528] [INFO] Task succeeded: Blastn
[2023-03-17 11:37:44,530] [INFO] Selected 30 target genomes.
[2023-03-17 11:37:44,530] [INFO] Target genome list was writen to OceanDNA-b37857/target_genomes_gtdb.txt
[2023-03-17 11:37:44,559] [INFO] Task started: fastANI
[2023-03-17 11:37:44,559] [INFO] Running command: fastANI --query /var/lib/cwl/stg9321edaf-c8b2-4915-aa5f-143c53448199/OceanDNA-b37857.fa --refList OceanDNA-b37857/target_genomes_gtdb.txt --output OceanDNA-b37857/fastani_result_gtdb.tsv --threads 1
[2023-03-17 11:37:58,735] [INFO] Task succeeded: fastANI
[2023-03-17 11:37:58,736] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-17 11:37:58,737] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002456975.1	s__Luminiphilus sp002456975	83.1411	163	223	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	99.72	99.71	0.90	0.87	3	-
--------------------------------------------------------------------------------
[2023-03-17 11:37:58,738] [INFO] GTDB search result was written to OceanDNA-b37857/result_gtdb.tsv
[2023-03-17 11:37:58,739] [INFO] ===== GTDB Search completed =====
[2023-03-17 11:37:58,741] [INFO] DFAST_QC result json was written to OceanDNA-b37857/dqc_result.json
[2023-03-17 11:37:58,741] [INFO] DFAST_QC completed!
[2023-03-17 11:37:58,741] [INFO] Total running time: 0h1m5s