[2023-03-15 05:29:12,457] [INFO] DFAST_QC pipeline started.
[2023-03-15 05:29:12,457] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 05:29:12,457] [INFO] DQC Reference Directory: /var/lib/cwl/stgcf62417a-5535-411d-8215-678684690f11/dqc_reference
[2023-03-15 05:29:13,544] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 05:29:13,545] [INFO] Task started: Prodigal
[2023-03-15 05:29:13,545] [INFO] Running command: cat /var/lib/cwl/stg533d09c7-284b-461c-89e6-4595b131fcb6/OceanDNA-b37960.fa | prodigal -d OceanDNA-b37960/cds.fna -a OceanDNA-b37960/protein.faa -g 11 -q > /dev/null
[2023-03-15 05:29:21,693] [INFO] Task succeeded: Prodigal
[2023-03-15 05:29:21,693] [INFO] Task started: HMMsearch
[2023-03-15 05:29:21,693] [INFO] Running command: hmmsearch --tblout OceanDNA-b37960/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgcf62417a-5535-411d-8215-678684690f11/dqc_reference/reference_markers.hmm OceanDNA-b37960/protein.faa > /dev/null
[2023-03-15 05:29:21,872] [INFO] Task succeeded: HMMsearch
[2023-03-15 05:29:21,873] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg533d09c7-284b-461c-89e6-4595b131fcb6/OceanDNA-b37960.fa]
[2023-03-15 05:29:21,884] [INFO] Query marker FASTA was written to OceanDNA-b37960/markers.fasta
[2023-03-15 05:29:21,884] [INFO] Task started: Blastn
[2023-03-15 05:29:21,884] [INFO] Running command: blastn -query OceanDNA-b37960/markers.fasta -db /var/lib/cwl/stgcf62417a-5535-411d-8215-678684690f11/dqc_reference/reference_markers.fasta -out OceanDNA-b37960/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:29:22,474] [INFO] Task succeeded: Blastn
[2023-03-15 05:29:22,475] [INFO] Selected 30 target genomes.
[2023-03-15 05:29:22,475] [INFO] Target genome list was writen to OceanDNA-b37960/target_genomes.txt
[2023-03-15 05:29:22,490] [INFO] Task started: fastANI
[2023-03-15 05:29:22,490] [INFO] Running command: fastANI --query /var/lib/cwl/stg533d09c7-284b-461c-89e6-4595b131fcb6/OceanDNA-b37960.fa --refList OceanDNA-b37960/target_genomes.txt --output OceanDNA-b37960/fastani_result.tsv --threads 1
[2023-03-15 05:29:41,342] [INFO] Task succeeded: fastANI
[2023-03-15 05:29:41,342] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgcf62417a-5535-411d-8215-678684690f11/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 05:29:41,343] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgcf62417a-5535-411d-8215-678684690f11/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 05:29:41,343] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 05:29:41,343] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 05:29:41,343] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 05:29:41,343] [INFO] DFAST Taxonomy check result was written to OceanDNA-b37960/tc_result.tsv
[2023-03-15 05:29:41,343] [INFO] ===== Taxonomy check completed =====
[2023-03-15 05:29:41,343] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 05:29:41,343] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgcf62417a-5535-411d-8215-678684690f11/dqc_reference/checkm_data
[2023-03-15 05:29:41,346] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 05:29:41,349] [INFO] Task started: CheckM
[2023-03-15 05:29:41,349] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b37960/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b37960/checkm_input OceanDNA-b37960/checkm_result
[2023-03-15 05:30:07,063] [INFO] Task succeeded: CheckM
[2023-03-15 05:30:07,063] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 81.04%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 05:30:07,065] [INFO] ===== Completeness check finished =====
[2023-03-15 05:30:07,065] [INFO] ===== Start GTDB Search =====
[2023-03-15 05:30:07,065] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b37960/markers.fasta)
[2023-03-15 05:30:07,065] [INFO] Task started: Blastn
[2023-03-15 05:30:07,065] [INFO] Running command: blastn -query OceanDNA-b37960/markers.fasta -db /var/lib/cwl/stgcf62417a-5535-411d-8215-678684690f11/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b37960/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:30:08,057] [INFO] Task succeeded: Blastn
[2023-03-15 05:30:08,058] [INFO] Selected 17 target genomes.
[2023-03-15 05:30:08,058] [INFO] Target genome list was writen to OceanDNA-b37960/target_genomes_gtdb.txt
[2023-03-15 05:30:08,072] [INFO] Task started: fastANI
[2023-03-15 05:30:08,073] [INFO] Running command: fastANI --query /var/lib/cwl/stg533d09c7-284b-461c-89e6-4595b131fcb6/OceanDNA-b37960.fa --refList OceanDNA-b37960/target_genomes_gtdb.txt --output OceanDNA-b37960/fastani_result_gtdb.tsv --threads 1
[2023-03-15 05:30:15,516] [INFO] Task succeeded: fastANI
[2023-03-15 05:30:15,521] [INFO] Found 8 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 05:30:15,522] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_000227505.4	s__Luminiphilus sp000227505	86.8224	409	446	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002689915.1	s__Luminiphilus sp002689915	78.5386	151	446	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	100.00	100.00	0.97	0.97	2	-
GCA_003523185.1	s__Luminiphilus sp003523185	77.9738	86	446	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902547755.1	s__Luminiphilus sp902547755	77.7297	52	446	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002691565.1	s__Luminiphilus sp002691565	77.3152	60	446	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	99.50	98.99	0.85	0.71	3	-
GCA_002862405.1	s__Luminiphilus sp002862405	77.3112	68	446	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	95.70	95.58	0.78	0.76	4	-
GCA_009937065.1	s__Luminiphilus sp009937065	76.7987	74	446	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002469785.1	s__Luminiphilus sp002469785	76.4995	56	446	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halieaceae;g__Luminiphilus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 05:30:15,522] [INFO] GTDB search result was written to OceanDNA-b37960/result_gtdb.tsv
[2023-03-15 05:30:15,522] [INFO] ===== GTDB Search completed =====
[2023-03-15 05:30:15,523] [INFO] DFAST_QC result json was written to OceanDNA-b37960/dqc_result.json
[2023-03-15 05:30:15,523] [INFO] DFAST_QC completed!
[2023-03-15 05:30:15,523] [INFO] Total running time: 0h1m3s