[2023-03-16 17:47:08,945] [INFO] DFAST_QC pipeline started.
[2023-03-16 17:47:08,946] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 17:47:08,946] [INFO] DQC Reference Directory: /var/lib/cwl/stg6b3446fa-3672-4c32-97fd-bdc829a1bb82/dqc_reference
[2023-03-16 17:47:10,996] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 17:47:10,996] [INFO] Task started: Prodigal
[2023-03-16 17:47:10,997] [INFO] Running command: cat /var/lib/cwl/stgf6ff5056-a7e8-404f-a5a6-98c9accb0845/OceanDNA-b38357.fa | prodigal -d OceanDNA-b38357/cds.fna -a OceanDNA-b38357/protein.faa -g 11 -q > /dev/null
[2023-03-16 17:47:16,202] [INFO] Task succeeded: Prodigal
[2023-03-16 17:47:16,202] [INFO] Task started: HMMsearch
[2023-03-16 17:47:16,202] [INFO] Running command: hmmsearch --tblout OceanDNA-b38357/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6b3446fa-3672-4c32-97fd-bdc829a1bb82/dqc_reference/reference_markers.hmm OceanDNA-b38357/protein.faa > /dev/null
[2023-03-16 17:47:16,399] [INFO] Task succeeded: HMMsearch
[2023-03-16 17:47:16,399] [INFO] Found 6/6 markers.
[2023-03-16 17:47:16,414] [INFO] Query marker FASTA was written to OceanDNA-b38357/markers.fasta
[2023-03-16 17:47:16,415] [INFO] Task started: Blastn
[2023-03-16 17:47:16,415] [INFO] Running command: blastn -query OceanDNA-b38357/markers.fasta -db /var/lib/cwl/stg6b3446fa-3672-4c32-97fd-bdc829a1bb82/dqc_reference/reference_markers.fasta -out OceanDNA-b38357/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 17:47:20,205] [INFO] Task succeeded: Blastn
[2023-03-16 17:47:20,207] [INFO] Selected 32 target genomes.
[2023-03-16 17:47:20,207] [INFO] Target genome list was writen to OceanDNA-b38357/target_genomes.txt
[2023-03-16 17:47:20,243] [INFO] Task started: fastANI
[2023-03-16 17:47:20,243] [INFO] Running command: fastANI --query /var/lib/cwl/stgf6ff5056-a7e8-404f-a5a6-98c9accb0845/OceanDNA-b38357.fa --refList OceanDNA-b38357/target_genomes.txt --output OceanDNA-b38357/fastani_result.tsv --threads 1
[2023-03-16 17:47:39,547] [INFO] Task succeeded: fastANI
[2023-03-16 17:47:39,547] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6b3446fa-3672-4c32-97fd-bdc829a1bb82/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 17:47:39,547] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6b3446fa-3672-4c32-97fd-bdc829a1bb82/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 17:47:39,547] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 17:47:39,548] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 17:47:39,548] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 17:47:39,548] [INFO] DFAST Taxonomy check result was written to OceanDNA-b38357/tc_result.tsv
[2023-03-16 17:47:39,549] [INFO] ===== Taxonomy check completed =====
[2023-03-16 17:47:39,549] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 17:47:39,549] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6b3446fa-3672-4c32-97fd-bdc829a1bb82/dqc_reference/checkm_data
[2023-03-16 17:47:39,560] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 17:47:39,564] [INFO] Task started: CheckM
[2023-03-16 17:47:39,564] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b38357/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b38357/checkm_input OceanDNA-b38357/checkm_result
[2023-03-16 17:47:59,259] [INFO] Task succeeded: CheckM
[2023-03-16 17:47:59,259] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 79.19%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 17:47:59,264] [INFO] ===== Completeness check finished =====
[2023-03-16 17:47:59,265] [INFO] ===== Start GTDB Search =====
[2023-03-16 17:47:59,265] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b38357/markers.fasta)
[2023-03-16 17:47:59,266] [INFO] Task started: Blastn
[2023-03-16 17:47:59,266] [INFO] Running command: blastn -query OceanDNA-b38357/markers.fasta -db /var/lib/cwl/stg6b3446fa-3672-4c32-97fd-bdc829a1bb82/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b38357/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 17:48:00,290] [INFO] Task succeeded: Blastn
[2023-03-16 17:48:00,292] [INFO] Selected 34 target genomes.
[2023-03-16 17:48:00,292] [INFO] Target genome list was writen to OceanDNA-b38357/target_genomes_gtdb.txt
[2023-03-16 17:48:00,357] [INFO] Task started: fastANI
[2023-03-16 17:48:00,357] [INFO] Running command: fastANI --query /var/lib/cwl/stgf6ff5056-a7e8-404f-a5a6-98c9accb0845/OceanDNA-b38357.fa --refList OceanDNA-b38357/target_genomes_gtdb.txt --output OceanDNA-b38357/fastani_result_gtdb.tsv --threads 1
[2023-03-16 17:48:28,664] [INFO] Task succeeded: fastANI
[2023-03-16 17:48:28,665] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 17:48:28,665] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-16 17:48:28,666] [INFO] GTDB search result was written to OceanDNA-b38357/result_gtdb.tsv
[2023-03-16 17:48:28,667] [INFO] ===== GTDB Search completed =====
[2023-03-16 17:48:28,668] [INFO] DFAST_QC result json was written to OceanDNA-b38357/dqc_result.json
[2023-03-16 17:48:28,668] [INFO] DFAST_QC completed!
[2023-03-16 17:48:28,668] [INFO] Total running time: 0h1m20s