[2023-03-15 03:56:21,754] [INFO] DFAST_QC pipeline started.
[2023-03-15 03:56:21,754] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 03:56:21,754] [INFO] DQC Reference Directory: /var/lib/cwl/stg8efac821-9a79-4ca3-9db2-45cdcc0d1c22/dqc_reference
[2023-03-15 03:56:23,400] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 03:56:23,401] [INFO] Task started: Prodigal
[2023-03-15 03:56:23,401] [INFO] Running command: cat /var/lib/cwl/stg0b3e5f8f-c278-4602-9373-9c02d0bcc333/OceanDNA-b38692.fa | prodigal -d OceanDNA-b38692/cds.fna -a OceanDNA-b38692/protein.faa -g 11 -q > /dev/null
[2023-03-15 03:56:37,612] [INFO] Task succeeded: Prodigal
[2023-03-15 03:56:37,612] [INFO] Task started: HMMsearch
[2023-03-15 03:56:37,612] [INFO] Running command: hmmsearch --tblout OceanDNA-b38692/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8efac821-9a79-4ca3-9db2-45cdcc0d1c22/dqc_reference/reference_markers.hmm OceanDNA-b38692/protein.faa > /dev/null
[2023-03-15 03:56:37,835] [INFO] Task succeeded: HMMsearch
[2023-03-15 03:56:37,835] [INFO] Found 6/6 markers.
[2023-03-15 03:56:37,851] [INFO] Query marker FASTA was written to OceanDNA-b38692/markers.fasta
[2023-03-15 03:56:37,851] [INFO] Task started: Blastn
[2023-03-15 03:56:37,851] [INFO] Running command: blastn -query OceanDNA-b38692/markers.fasta -db /var/lib/cwl/stg8efac821-9a79-4ca3-9db2-45cdcc0d1c22/dqc_reference/reference_markers.fasta -out OceanDNA-b38692/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 03:56:38,415] [INFO] Task succeeded: Blastn
[2023-03-15 03:56:38,416] [INFO] Selected 35 target genomes.
[2023-03-15 03:56:38,416] [INFO] Target genome list was writen to OceanDNA-b38692/target_genomes.txt
[2023-03-15 03:56:38,434] [INFO] Task started: fastANI
[2023-03-15 03:56:38,435] [INFO] Running command: fastANI --query /var/lib/cwl/stg0b3e5f8f-c278-4602-9373-9c02d0bcc333/OceanDNA-b38692.fa --refList OceanDNA-b38692/target_genomes.txt --output OceanDNA-b38692/fastani_result.tsv --threads 1
[2023-03-15 03:56:59,658] [INFO] Task succeeded: fastANI
[2023-03-15 03:56:59,659] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8efac821-9a79-4ca3-9db2-45cdcc0d1c22/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 03:56:59,659] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8efac821-9a79-4ca3-9db2-45cdcc0d1c22/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 03:56:59,659] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 03:56:59,659] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 03:56:59,659] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 03:56:59,659] [INFO] DFAST Taxonomy check result was written to OceanDNA-b38692/tc_result.tsv
[2023-03-15 03:56:59,660] [INFO] ===== Taxonomy check completed =====
[2023-03-15 03:56:59,660] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 03:56:59,660] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8efac821-9a79-4ca3-9db2-45cdcc0d1c22/dqc_reference/checkm_data
[2023-03-15 03:56:59,662] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 03:56:59,769] [INFO] Task started: CheckM
[2023-03-15 03:56:59,769] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b38692/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b38692/checkm_input OceanDNA-b38692/checkm_result
[2023-03-15 03:57:38,030] [INFO] Task succeeded: CheckM
[2023-03-15 03:57:38,031] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 89.68%
Contamintation: 0.52%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-15 03:57:38,034] [INFO] ===== Completeness check finished =====
[2023-03-15 03:57:38,034] [INFO] ===== Start GTDB Search =====
[2023-03-15 03:57:38,034] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b38692/markers.fasta)
[2023-03-15 03:57:38,035] [INFO] Task started: Blastn
[2023-03-15 03:57:38,036] [INFO] Running command: blastn -query OceanDNA-b38692/markers.fasta -db /var/lib/cwl/stg8efac821-9a79-4ca3-9db2-45cdcc0d1c22/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b38692/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 03:57:38,782] [INFO] Task succeeded: Blastn
[2023-03-15 03:57:38,783] [INFO] Selected 13 target genomes.
[2023-03-15 03:57:38,783] [INFO] Target genome list was writen to OceanDNA-b38692/target_genomes_gtdb.txt
[2023-03-15 03:57:38,796] [INFO] Task started: fastANI
[2023-03-15 03:57:38,796] [INFO] Running command: fastANI --query /var/lib/cwl/stg0b3e5f8f-c278-4602-9373-9c02d0bcc333/OceanDNA-b38692.fa --refList OceanDNA-b38692/target_genomes_gtdb.txt --output OceanDNA-b38692/fastani_result_gtdb.tsv --threads 1
[2023-03-15 03:57:45,650] [INFO] Task succeeded: fastANI
[2023-03-15 03:57:45,655] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-15 03:57:45,655] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_905182035.1	s__ASP10-02a sp905182035	99.609	617	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Nitrincolaceae;g__ASP10-02a	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_002312935.1	s__ASP10-02a sp002312935	81.6355	499	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Nitrincolaceae;g__ASP10-02a	95.0	99.42	99.42	0.91	0.91	2	-
GCA_002335115.1	s__ASP10-02a sp002335115	79.3926	378	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Nitrincolaceae;g__ASP10-02a	95.0	99.15	95.37	0.85	0.79	13	-
GCA_002470565.1	s__ASP10-02a sp002470565	79.1537	345	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Nitrincolaceae;g__ASP10-02a	95.0	99.72	99.72	0.90	0.90	2	-
GCA_018647765.1	s__ASP10-02a sp018647765	78.9904	249	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Nitrincolaceae;g__ASP10-02a	95.0	99.86	99.85	0.95	0.93	3	-
GCA_003523085.1	s__ASP10-02a sp003523085	78.2739	253	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Nitrincolaceae;g__ASP10-02a	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 03:57:45,655] [INFO] GTDB search result was written to OceanDNA-b38692/result_gtdb.tsv
[2023-03-15 03:57:45,655] [INFO] ===== GTDB Search completed =====
[2023-03-15 03:57:45,656] [INFO] DFAST_QC result json was written to OceanDNA-b38692/dqc_result.json
[2023-03-15 03:57:45,656] [INFO] DFAST_QC completed!
[2023-03-15 03:57:45,656] [INFO] Total running time: 0h1m24s