[2023-03-15 03:56:21,754] [INFO] DFAST_QC pipeline started.
[2023-03-15 03:56:21,754] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 03:56:21,754] [INFO] DQC Reference Directory: /var/lib/cwl/stg47142581-dfac-43e2-a10b-6d19de435851/dqc_reference
[2023-03-15 03:56:23,400] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 03:56:23,401] [INFO] Task started: Prodigal
[2023-03-15 03:56:23,401] [INFO] Running command: cat /var/lib/cwl/stgf017140d-a9bb-4f22-afdc-f806a113cced/OceanDNA-b3927.fa | prodigal -d OceanDNA-b3927/cds.fna -a OceanDNA-b3927/protein.faa -g 11 -q > /dev/null
[2023-03-15 03:57:03,422] [INFO] Task succeeded: Prodigal
[2023-03-15 03:57:03,422] [INFO] Task started: HMMsearch
[2023-03-15 03:57:03,422] [INFO] Running command: hmmsearch --tblout OceanDNA-b3927/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg47142581-dfac-43e2-a10b-6d19de435851/dqc_reference/reference_markers.hmm OceanDNA-b3927/protein.faa > /dev/null
[2023-03-15 03:57:03,711] [INFO] Task succeeded: HMMsearch
[2023-03-15 03:57:03,712] [INFO] Found 6/6 markers.
[2023-03-15 03:57:03,750] [INFO] Query marker FASTA was written to OceanDNA-b3927/markers.fasta
[2023-03-15 03:57:03,750] [INFO] Task started: Blastn
[2023-03-15 03:57:03,750] [INFO] Running command: blastn -query OceanDNA-b3927/markers.fasta -db /var/lib/cwl/stg47142581-dfac-43e2-a10b-6d19de435851/dqc_reference/reference_markers.fasta -out OceanDNA-b3927/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 03:57:04,287] [INFO] Task succeeded: Blastn
[2023-03-15 03:57:04,288] [INFO] Selected 26 target genomes.
[2023-03-15 03:57:04,288] [INFO] Target genome list was writen to OceanDNA-b3927/target_genomes.txt
[2023-03-15 03:57:04,302] [INFO] Task started: fastANI
[2023-03-15 03:57:04,302] [INFO] Running command: fastANI --query /var/lib/cwl/stgf017140d-a9bb-4f22-afdc-f806a113cced/OceanDNA-b3927.fa --refList OceanDNA-b3927/target_genomes.txt --output OceanDNA-b3927/fastani_result.tsv --threads 1
[2023-03-15 03:57:22,018] [INFO] Task succeeded: fastANI
[2023-03-15 03:57:22,019] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg47142581-dfac-43e2-a10b-6d19de435851/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 03:57:22,019] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg47142581-dfac-43e2-a10b-6d19de435851/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 03:57:22,022] [INFO] Found 1 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 03:57:22,022] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-15 03:57:22,022] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Marinoscillum pacificum	strain=MRN461	GCA_024714495.1	392723	392723	type	True	75.108	51	1915	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-15 03:57:22,023] [INFO] DFAST Taxonomy check result was written to OceanDNA-b3927/tc_result.tsv
[2023-03-15 03:57:22,023] [INFO] ===== Taxonomy check completed =====
[2023-03-15 03:57:22,023] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 03:57:22,023] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg47142581-dfac-43e2-a10b-6d19de435851/dqc_reference/checkm_data
[2023-03-15 03:57:22,024] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 03:57:22,197] [INFO] Task started: CheckM
[2023-03-15 03:57:22,197] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b3927/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b3927/checkm_input OceanDNA-b3927/checkm_result
[2023-03-15 03:59:00,171] [INFO] Task succeeded: CheckM
[2023-03-15 03:59:00,171] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 62.50%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 03:59:00,175] [INFO] ===== Completeness check finished =====
[2023-03-15 03:59:00,176] [INFO] ===== Start GTDB Search =====
[2023-03-15 03:59:00,176] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b3927/markers.fasta)
[2023-03-15 03:59:00,177] [INFO] Task started: Blastn
[2023-03-15 03:59:00,177] [INFO] Running command: blastn -query OceanDNA-b3927/markers.fasta -db /var/lib/cwl/stg47142581-dfac-43e2-a10b-6d19de435851/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b3927/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 03:59:00,917] [INFO] Task succeeded: Blastn
[2023-03-15 03:59:00,918] [INFO] Selected 27 target genomes.
[2023-03-15 03:59:00,918] [INFO] Target genome list was writen to OceanDNA-b3927/target_genomes_gtdb.txt
[2023-03-15 03:59:00,944] [INFO] Task started: fastANI
[2023-03-15 03:59:00,945] [INFO] Running command: fastANI --query /var/lib/cwl/stgf017140d-a9bb-4f22-afdc-f806a113cced/OceanDNA-b3927.fa --refList OceanDNA-b3927/target_genomes_gtdb.txt --output OceanDNA-b3927/fastani_result_gtdb.tsv --threads 1
[2023-03-15 03:59:21,439] [INFO] Task succeeded: fastANI
[2023-03-15 03:59:21,440] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 03:59:21,440] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-15 03:59:21,440] [INFO] GTDB search result was written to OceanDNA-b3927/result_gtdb.tsv
[2023-03-15 03:59:21,440] [INFO] ===== GTDB Search completed =====
[2023-03-15 03:59:21,440] [INFO] DFAST_QC result json was written to OceanDNA-b3927/dqc_result.json
[2023-03-15 03:59:21,441] [INFO] DFAST_QC completed!
[2023-03-15 03:59:21,441] [INFO] Total running time: 0h2m60s