[2023-03-16 06:33:31,978] [INFO] DFAST_QC pipeline started.
[2023-03-16 06:33:31,978] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 06:33:31,978] [INFO] DQC Reference Directory: /var/lib/cwl/stg9e46cd1b-9bbb-4198-975e-a51f6353e410/dqc_reference
[2023-03-16 06:33:33,082] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 06:33:33,083] [INFO] Task started: Prodigal
[2023-03-16 06:33:33,083] [INFO] Running command: cat /var/lib/cwl/stg8eacc93f-b8cb-45aa-b82a-dc75e5f9f887/OceanDNA-b3981.fa | prodigal -d OceanDNA-b3981/cds.fna -a OceanDNA-b3981/protein.faa -g 11 -q > /dev/null
[2023-03-16 06:33:51,130] [INFO] Task succeeded: Prodigal
[2023-03-16 06:33:51,130] [INFO] Task started: HMMsearch
[2023-03-16 06:33:51,130] [INFO] Running command: hmmsearch --tblout OceanDNA-b3981/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9e46cd1b-9bbb-4198-975e-a51f6353e410/dqc_reference/reference_markers.hmm OceanDNA-b3981/protein.faa > /dev/null
[2023-03-16 06:33:51,325] [INFO] Task succeeded: HMMsearch
[2023-03-16 06:33:51,325] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg8eacc93f-b8cb-45aa-b82a-dc75e5f9f887/OceanDNA-b3981.fa]
[2023-03-16 06:33:51,348] [INFO] Query marker FASTA was written to OceanDNA-b3981/markers.fasta
[2023-03-16 06:33:51,349] [INFO] Task started: Blastn
[2023-03-16 06:33:51,349] [INFO] Running command: blastn -query OceanDNA-b3981/markers.fasta -db /var/lib/cwl/stg9e46cd1b-9bbb-4198-975e-a51f6353e410/dqc_reference/reference_markers.fasta -out OceanDNA-b3981/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 06:33:51,855] [INFO] Task succeeded: Blastn
[2023-03-16 06:33:51,856] [INFO] Selected 12 target genomes.
[2023-03-16 06:33:51,856] [INFO] Target genome list was writen to OceanDNA-b3981/target_genomes.txt
[2023-03-16 06:33:51,863] [INFO] Task started: fastANI
[2023-03-16 06:33:51,863] [INFO] Running command: fastANI --query /var/lib/cwl/stg8eacc93f-b8cb-45aa-b82a-dc75e5f9f887/OceanDNA-b3981.fa --refList OceanDNA-b3981/target_genomes.txt --output OceanDNA-b3981/fastani_result.tsv --threads 1
[2023-03-16 06:34:00,971] [INFO] Task succeeded: fastANI
[2023-03-16 06:34:00,971] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9e46cd1b-9bbb-4198-975e-a51f6353e410/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 06:34:00,971] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9e46cd1b-9bbb-4198-975e-a51f6353e410/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 06:34:00,977] [INFO] Found 7 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 06:34:00,977] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-16 06:34:00,977] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Algoriphagus yeomjeoni	strain=DSM 23446	GCA_003259505.1	291403	291403	type	True	81.8661	466	714	95	below_threshold
Algoriphagus locisalis	strain=DSM 23445	GCA_900116615.1	305507	305507	type	True	81.7768	464	714	95	below_threshold
Algoriphagus aquimarinus	strain=DSM 23399	GCA_900112005.1	237018	237018	type	True	79.9156	341	714	95	below_threshold
Algoriphagus chordae	strain=DSM 19830	GCA_003254055.1	237019	237019	type	True	79.552	327	714	95	below_threshold
Algoriphagus antarcticus	strain=DSM 15986	GCA_002150685.1	238540	238540	type	True	79.4367	322	714	95	below_threshold
Algoriphagus ratkowskyi	strain=DSM 22686	GCA_002150505.1	57028	57028	type	True	78.7788	275	714	95	below_threshold
Algoriphagus ratkowskyi	strain=DSM 22686	GCA_003254355.1	57028	57028	type	True	78.7387	276	714	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-16 06:34:00,977] [INFO] DFAST Taxonomy check result was written to OceanDNA-b3981/tc_result.tsv
[2023-03-16 06:34:00,977] [INFO] ===== Taxonomy check completed =====
[2023-03-16 06:34:00,977] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 06:34:00,977] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9e46cd1b-9bbb-4198-975e-a51f6353e410/dqc_reference/checkm_data
[2023-03-16 06:34:00,978] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 06:34:00,983] [INFO] Task started: CheckM
[2023-03-16 06:34:00,983] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b3981/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b3981/checkm_input OceanDNA-b3981/checkm_result
[2023-03-16 06:34:50,280] [INFO] Task succeeded: CheckM
[2023-03-16 06:34:50,280] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 37.50%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 06:34:50,283] [INFO] ===== Completeness check finished =====
[2023-03-16 06:34:50,283] [INFO] ===== Start GTDB Search =====
[2023-03-16 06:34:50,283] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b3981/markers.fasta)
[2023-03-16 06:34:50,283] [INFO] Task started: Blastn
[2023-03-16 06:34:50,283] [INFO] Running command: blastn -query OceanDNA-b3981/markers.fasta -db /var/lib/cwl/stg9e46cd1b-9bbb-4198-975e-a51f6353e410/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b3981/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 06:34:50,981] [INFO] Task succeeded: Blastn
[2023-03-16 06:34:50,982] [INFO] Selected 8 target genomes.
[2023-03-16 06:34:50,982] [INFO] Target genome list was writen to OceanDNA-b3981/target_genomes_gtdb.txt
[2023-03-16 06:34:50,990] [INFO] Task started: fastANI
[2023-03-16 06:34:50,990] [INFO] Running command: fastANI --query /var/lib/cwl/stg8eacc93f-b8cb-45aa-b82a-dc75e5f9f887/OceanDNA-b3981.fa --refList OceanDNA-b3981/target_genomes_gtdb.txt --output OceanDNA-b3981/fastani_result_gtdb.tsv --threads 1
[2023-03-16 06:34:57,859] [INFO] Task succeeded: fastANI
[2023-03-16 06:34:57,864] [INFO] Found 8 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 06:34:57,864] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003259505.1	s__Algoriphagus yeomjeoni	81.8661	466	714	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900116615.1	s__Algoriphagus locisalis	81.7768	464	714	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007997215.1	s__Algoriphagus aquimarinus_A	80.0553	359	714	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900112005.1	s__Algoriphagus aquimarinus	79.9312	340	714	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_017254845.1	s__Algoriphagus sp017254845	79.6816	164	714	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003254055.1	s__Algoriphagus chordae	79.5599	326	714	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014838715.1	s__Algoriphagus sp014838715	78.8312	299	714	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002150505.1	s__Algoriphagus ratkowskyi	78.7943	274	714	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__Algoriphagus	95.0	100.00	99.99	1.00	1.00	3	-
--------------------------------------------------------------------------------
[2023-03-16 06:34:57,864] [INFO] GTDB search result was written to OceanDNA-b3981/result_gtdb.tsv
[2023-03-16 06:34:57,864] [INFO] ===== GTDB Search completed =====
[2023-03-16 06:34:57,866] [INFO] DFAST_QC result json was written to OceanDNA-b3981/dqc_result.json
[2023-03-16 06:34:57,866] [INFO] DFAST_QC completed!
[2023-03-16 06:34:57,866] [INFO] Total running time: 0h1m26s