[2023-03-17 23:08:31,912] [INFO] DFAST_QC pipeline started.
[2023-03-17 23:08:31,912] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 23:08:31,912] [INFO] DQC Reference Directory: /var/lib/cwl/stg6c1849a2-4953-4787-921b-1f0d311c23b5/dqc_reference
[2023-03-17 23:08:33,079] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 23:08:33,079] [INFO] Task started: Prodigal
[2023-03-17 23:08:33,079] [INFO] Running command: cat /var/lib/cwl/stg4a2fa41c-7809-4b06-ae86-26fd307ea338/OceanDNA-b39854.fa | prodigal -d OceanDNA-b39854/cds.fna -a OceanDNA-b39854/protein.faa -g 11 -q > /dev/null
[2023-03-17 23:08:45,244] [INFO] Task succeeded: Prodigal
[2023-03-17 23:08:45,245] [INFO] Task started: HMMsearch
[2023-03-17 23:08:45,245] [INFO] Running command: hmmsearch --tblout OceanDNA-b39854/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6c1849a2-4953-4787-921b-1f0d311c23b5/dqc_reference/reference_markers.hmm OceanDNA-b39854/protein.faa > /dev/null
[2023-03-17 23:08:45,452] [INFO] Task succeeded: HMMsearch
[2023-03-17 23:08:45,453] [INFO] Found 6/6 markers.
[2023-03-17 23:08:45,470] [INFO] Query marker FASTA was written to OceanDNA-b39854/markers.fasta
[2023-03-17 23:08:45,471] [INFO] Task started: Blastn
[2023-03-17 23:08:45,471] [INFO] Running command: blastn -query OceanDNA-b39854/markers.fasta -db /var/lib/cwl/stg6c1849a2-4953-4787-921b-1f0d311c23b5/dqc_reference/reference_markers.fasta -out OceanDNA-b39854/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 23:08:46,077] [INFO] Task succeeded: Blastn
[2023-03-17 23:08:46,080] [INFO] Selected 29 target genomes.
[2023-03-17 23:08:46,080] [INFO] Target genome list was writen to OceanDNA-b39854/target_genomes.txt
[2023-03-17 23:08:46,096] [INFO] Task started: fastANI
[2023-03-17 23:08:46,096] [INFO] Running command: fastANI --query /var/lib/cwl/stg4a2fa41c-7809-4b06-ae86-26fd307ea338/OceanDNA-b39854.fa --refList OceanDNA-b39854/target_genomes.txt --output OceanDNA-b39854/fastani_result.tsv --threads 1
[2023-03-17 23:09:03,889] [INFO] Task succeeded: fastANI
[2023-03-17 23:09:03,890] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6c1849a2-4953-4787-921b-1f0d311c23b5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 23:09:03,890] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6c1849a2-4953-4787-921b-1f0d311c23b5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 23:09:03,890] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 23:09:03,890] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 23:09:03,890] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 23:09:03,894] [INFO] DFAST Taxonomy check result was written to OceanDNA-b39854/tc_result.tsv
[2023-03-17 23:09:03,899] [INFO] ===== Taxonomy check completed =====
[2023-03-17 23:09:03,899] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 23:09:03,899] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6c1849a2-4953-4787-921b-1f0d311c23b5/dqc_reference/checkm_data
[2023-03-17 23:09:03,903] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 23:09:03,943] [INFO] Task started: CheckM
[2023-03-17 23:09:03,943] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b39854/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b39854/checkm_input OceanDNA-b39854/checkm_result
[2023-03-17 23:09:37,889] [INFO] Task succeeded: CheckM
[2023-03-17 23:09:37,889] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.33%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-17 23:09:37,906] [INFO] ===== Completeness check finished =====
[2023-03-17 23:09:37,906] [INFO] ===== Start GTDB Search =====
[2023-03-17 23:09:37,906] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b39854/markers.fasta)
[2023-03-17 23:09:37,907] [INFO] Task started: Blastn
[2023-03-17 23:09:37,907] [INFO] Running command: blastn -query OceanDNA-b39854/markers.fasta -db /var/lib/cwl/stg6c1849a2-4953-4787-921b-1f0d311c23b5/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b39854/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 23:09:38,887] [INFO] Task succeeded: Blastn
[2023-03-17 23:09:38,891] [INFO] Selected 14 target genomes.
[2023-03-17 23:09:38,891] [INFO] Target genome list was writen to OceanDNA-b39854/target_genomes_gtdb.txt
[2023-03-17 23:09:38,902] [INFO] Task started: fastANI
[2023-03-17 23:09:38,903] [INFO] Running command: fastANI --query /var/lib/cwl/stg4a2fa41c-7809-4b06-ae86-26fd307ea338/OceanDNA-b39854.fa --refList OceanDNA-b39854/target_genomes_gtdb.txt --output OceanDNA-b39854/fastani_result_gtdb.tsv --threads 1
[2023-03-17 23:09:44,316] [INFO] Task succeeded: fastANI
[2023-03-17 23:09:44,324] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-17 23:09:44,324] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002335945.1	s__HTCC2207 sp002335945	99.7427	603	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	99.61	99.20	0.84	0.78	6	conclusive
GCA_905182245.1	s__HTCC2207 sp905182245	93.0531	326	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905182265.1	s__HTCC2207 sp905182265	79.6199	251	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	N/A	N/A	N/A	N/A	1	-
GCA_000511875.1	s__HTCC2207 sp000511875	78.2896	187	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905182855.1	s__HTCC2207 sp905182855	78.0355	172	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018664075.1	s__HTCC2207 sp018664075	78.0024	221	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	99.59	99.59	0.81	0.81	2	-
GCA_000153445.1	s__HTCC2207 sp000153445	77.92	198	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	98.85	98.71	0.89	0.85	4	-
GCA_002378645.1	s__HTCC2207 sp002378645	77.7152	196	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	99.56	99.15	0.89	0.85	12	-
GCA_002334915.1	s__HTCC2207 sp002334915	77.6597	213	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	99.52	99.52	0.87	0.87	2	-
GCA_905478725.1	s__HTCC2207 sp905478725	77.6174	157	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017858295.1	s__HTCC2207 sp017858295	77.5017	126	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	97.83	97.83	0.77	0.77	2	-
GCA_018663035.1	s__HTCC2207 sp018663035	76.9322	167	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	99.88	99.88	0.90	0.90	2	-
GCA_018607545.1	s__HTCC2207 sp018607545	76.8613	64	701	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-17 23:09:44,326] [INFO] GTDB search result was written to OceanDNA-b39854/result_gtdb.tsv
[2023-03-17 23:09:44,326] [INFO] ===== GTDB Search completed =====
[2023-03-17 23:09:44,328] [INFO] DFAST_QC result json was written to OceanDNA-b39854/dqc_result.json
[2023-03-17 23:09:44,329] [INFO] DFAST_QC completed!
[2023-03-17 23:09:44,329] [INFO] Total running time: 0h1m12s