[2023-03-18 20:40:39,435] [INFO] DFAST_QC pipeline started.
[2023-03-18 20:40:39,435] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 20:40:39,436] [INFO] DQC Reference Directory: /var/lib/cwl/stg04e825a4-803b-4bbb-8159-204f637b02e2/dqc_reference
[2023-03-18 20:40:41,214] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 20:40:41,214] [INFO] Task started: Prodigal
[2023-03-18 20:40:41,214] [INFO] Running command: cat /var/lib/cwl/stgf0884b25-1249-4d69-9f31-a0227c01a4a4/OceanDNA-b40018.fa | prodigal -d OceanDNA-b40018/cds.fna -a OceanDNA-b40018/protein.faa -g 11 -q > /dev/null
[2023-03-18 20:40:49,788] [INFO] Task succeeded: Prodigal
[2023-03-18 20:40:49,788] [INFO] Task started: HMMsearch
[2023-03-18 20:40:49,788] [INFO] Running command: hmmsearch --tblout OceanDNA-b40018/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg04e825a4-803b-4bbb-8159-204f637b02e2/dqc_reference/reference_markers.hmm OceanDNA-b40018/protein.faa > /dev/null
[2023-03-18 20:40:50,016] [INFO] Task succeeded: HMMsearch
[2023-03-18 20:40:50,017] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgf0884b25-1249-4d69-9f31-a0227c01a4a4/OceanDNA-b40018.fa]
[2023-03-18 20:40:50,032] [INFO] Query marker FASTA was written to OceanDNA-b40018/markers.fasta
[2023-03-18 20:40:50,032] [INFO] Task started: Blastn
[2023-03-18 20:40:50,032] [INFO] Running command: blastn -query OceanDNA-b40018/markers.fasta -db /var/lib/cwl/stg04e825a4-803b-4bbb-8159-204f637b02e2/dqc_reference/reference_markers.fasta -out OceanDNA-b40018/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 20:40:50,590] [INFO] Task succeeded: Blastn
[2023-03-18 20:40:50,591] [INFO] Selected 24 target genomes.
[2023-03-18 20:40:50,591] [INFO] Target genome list was writen to OceanDNA-b40018/target_genomes.txt
[2023-03-18 20:40:50,603] [INFO] Task started: fastANI
[2023-03-18 20:40:50,603] [INFO] Running command: fastANI --query /var/lib/cwl/stgf0884b25-1249-4d69-9f31-a0227c01a4a4/OceanDNA-b40018.fa --refList OceanDNA-b40018/target_genomes.txt --output OceanDNA-b40018/fastani_result.tsv --threads 1
[2023-03-18 20:41:07,272] [INFO] Task succeeded: fastANI
[2023-03-18 20:41:07,273] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg04e825a4-803b-4bbb-8159-204f637b02e2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 20:41:07,273] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg04e825a4-803b-4bbb-8159-204f637b02e2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 20:41:07,273] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 20:41:07,273] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 20:41:07,273] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 20:41:07,273] [INFO] DFAST Taxonomy check result was written to OceanDNA-b40018/tc_result.tsv
[2023-03-18 20:41:07,273] [INFO] ===== Taxonomy check completed =====
[2023-03-18 20:41:07,274] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 20:41:07,274] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg04e825a4-803b-4bbb-8159-204f637b02e2/dqc_reference/checkm_data
[2023-03-18 20:41:07,277] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 20:41:07,280] [INFO] Task started: CheckM
[2023-03-18 20:41:07,280] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b40018/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b40018/checkm_input OceanDNA-b40018/checkm_result
[2023-03-18 20:41:34,048] [INFO] Task succeeded: CheckM
[2023-03-18 20:41:34,048] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 64.41%
Contamintation: 5.21%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-18 20:41:34,051] [INFO] ===== Completeness check finished =====
[2023-03-18 20:41:34,051] [INFO] ===== Start GTDB Search =====
[2023-03-18 20:41:34,051] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b40018/markers.fasta)
[2023-03-18 20:41:34,051] [INFO] Task started: Blastn
[2023-03-18 20:41:34,051] [INFO] Running command: blastn -query OceanDNA-b40018/markers.fasta -db /var/lib/cwl/stg04e825a4-803b-4bbb-8159-204f637b02e2/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b40018/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 20:41:34,903] [INFO] Task succeeded: Blastn
[2023-03-18 20:41:34,904] [INFO] Selected 16 target genomes.
[2023-03-18 20:41:34,904] [INFO] Target genome list was writen to OceanDNA-b40018/target_genomes_gtdb.txt
[2023-03-18 20:41:34,945] [INFO] Task started: fastANI
[2023-03-18 20:41:34,946] [INFO] Running command: fastANI --query /var/lib/cwl/stgf0884b25-1249-4d69-9f31-a0227c01a4a4/OceanDNA-b40018.fa --refList OceanDNA-b40018/target_genomes_gtdb.txt --output OceanDNA-b40018/fastani_result_gtdb.tsv --threads 1
[2023-03-18 20:41:42,300] [INFO] Task succeeded: fastANI
[2023-03-18 20:41:42,306] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-18 20:41:42,306] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002685715.1	s__HTCC2207 sp002685715	99.3209	376	462	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_018664075.1	s__HTCC2207 sp018664075	77.6518	146	462	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	99.59	99.59	0.81	0.81	2	-
GCA_002378645.1	s__HTCC2207 sp002378645	77.5563	108	462	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	99.56	99.15	0.89	0.85	12	-
GCA_002334915.1	s__HTCC2207 sp002334915	77.2933	107	462	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	99.52	99.52	0.87	0.87	2	-
GCA_018663035.1	s__HTCC2207 sp018663035	77.1117	75	462	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	99.88	99.88	0.90	0.90	2	-
GCA_000153445.1	s__HTCC2207 sp000153445	76.5919	68	462	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	98.85	98.71	0.89	0.85	4	-
GCA_000511875.1	s__HTCC2207 sp000511875	76.2818	59	462	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017858135.1	s__HTCC2207 sp017858135	75.9447	55	462	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Porticoccaceae;g__HTCC2207	95.0	98.96	98.94	0.87	0.86	3	-
--------------------------------------------------------------------------------
[2023-03-18 20:41:42,307] [INFO] GTDB search result was written to OceanDNA-b40018/result_gtdb.tsv
[2023-03-18 20:41:42,307] [INFO] ===== GTDB Search completed =====
[2023-03-18 20:41:42,307] [INFO] DFAST_QC result json was written to OceanDNA-b40018/dqc_result.json
[2023-03-18 20:41:42,308] [INFO] DFAST_QC completed!
[2023-03-18 20:41:42,308] [INFO] Total running time: 0h1m3s