[2023-03-15 16:47:24,730] [INFO] DFAST_QC pipeline started.
[2023-03-15 16:47:24,732] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 16:47:24,732] [INFO] DQC Reference Directory: /var/lib/cwl/stg162f0123-29a1-4752-ab42-04fd165ddb74/dqc_reference
[2023-03-15 16:47:27,249] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 16:47:27,250] [INFO] Task started: Prodigal
[2023-03-15 16:47:27,250] [INFO] Running command: cat /var/lib/cwl/stg829c6910-1434-4b9f-b5b8-ec23e9d38bbb/OceanDNA-b40495.fa | prodigal -d OceanDNA-b40495/cds.fna -a OceanDNA-b40495/protein.faa -g 11 -q > /dev/null
[2023-03-15 16:47:43,259] [INFO] Task succeeded: Prodigal
[2023-03-15 16:47:43,259] [INFO] Task started: HMMsearch
[2023-03-15 16:47:43,259] [INFO] Running command: hmmsearch --tblout OceanDNA-b40495/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg162f0123-29a1-4752-ab42-04fd165ddb74/dqc_reference/reference_markers.hmm OceanDNA-b40495/protein.faa > /dev/null
[2023-03-15 16:47:43,606] [INFO] Task succeeded: HMMsearch
[2023-03-15 16:47:43,606] [INFO] Found 6/6 markers.
[2023-03-15 16:47:43,627] [INFO] Query marker FASTA was written to OceanDNA-b40495/markers.fasta
[2023-03-15 16:47:43,629] [INFO] Task started: Blastn
[2023-03-15 16:47:43,629] [INFO] Running command: blastn -query OceanDNA-b40495/markers.fasta -db /var/lib/cwl/stg162f0123-29a1-4752-ab42-04fd165ddb74/dqc_reference/reference_markers.fasta -out OceanDNA-b40495/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 16:47:44,251] [INFO] Task succeeded: Blastn
[2023-03-15 16:47:44,253] [INFO] Selected 28 target genomes.
[2023-03-15 16:47:44,253] [INFO] Target genome list was writen to OceanDNA-b40495/target_genomes.txt
[2023-03-15 16:47:44,290] [INFO] Task started: fastANI
[2023-03-15 16:47:44,290] [INFO] Running command: fastANI --query /var/lib/cwl/stg829c6910-1434-4b9f-b5b8-ec23e9d38bbb/OceanDNA-b40495.fa --refList OceanDNA-b40495/target_genomes.txt --output OceanDNA-b40495/fastani_result.tsv --threads 1
[2023-03-15 16:48:06,383] [INFO] Task succeeded: fastANI
[2023-03-15 16:48:06,384] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg162f0123-29a1-4752-ab42-04fd165ddb74/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 16:48:06,384] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg162f0123-29a1-4752-ab42-04fd165ddb74/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 16:48:06,384] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 16:48:06,384] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 16:48:06,384] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 16:48:06,386] [INFO] DFAST Taxonomy check result was written to OceanDNA-b40495/tc_result.tsv
[2023-03-15 16:48:06,386] [INFO] ===== Taxonomy check completed =====
[2023-03-15 16:48:06,386] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 16:48:06,386] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg162f0123-29a1-4752-ab42-04fd165ddb74/dqc_reference/checkm_data
[2023-03-15 16:48:06,389] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 16:48:06,463] [INFO] Task started: CheckM
[2023-03-15 16:48:06,463] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b40495/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b40495/checkm_input OceanDNA-b40495/checkm_result
[2023-03-15 16:48:49,255] [INFO] Task succeeded: CheckM
[2023-03-15 16:48:49,255] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-15 16:48:49,298] [INFO] ===== Completeness check finished =====
[2023-03-15 16:48:49,298] [INFO] ===== Start GTDB Search =====
[2023-03-15 16:48:49,299] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b40495/markers.fasta)
[2023-03-15 16:48:49,300] [INFO] Task started: Blastn
[2023-03-15 16:48:49,300] [INFO] Running command: blastn -query OceanDNA-b40495/markers.fasta -db /var/lib/cwl/stg162f0123-29a1-4752-ab42-04fd165ddb74/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b40495/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 16:48:50,300] [INFO] Task succeeded: Blastn
[2023-03-15 16:48:50,307] [INFO] Selected 30 target genomes.
[2023-03-15 16:48:50,307] [INFO] Target genome list was writen to OceanDNA-b40495/target_genomes_gtdb.txt
[2023-03-15 16:48:50,359] [INFO] Task started: fastANI
[2023-03-15 16:48:50,359] [INFO] Running command: fastANI --query /var/lib/cwl/stg829c6910-1434-4b9f-b5b8-ec23e9d38bbb/OceanDNA-b40495.fa --refList OceanDNA-b40495/target_genomes_gtdb.txt --output OceanDNA-b40495/fastani_result_gtdb.tsv --threads 1
[2023-03-15 16:49:07,081] [INFO] Task succeeded: fastANI
[2023-03-15 16:49:07,083] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 16:49:07,084] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002746215.1	s__UBA5109 sp002746215	77.5101	190	921	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudohongiellaceae;g__UBA5109	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002414385.1	s__UBA5109 sp002414385	77.491	157	921	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudohongiellaceae;g__UBA5109	95.0	99.32	99.09	0.94	0.90	5	-
--------------------------------------------------------------------------------
[2023-03-15 16:49:07,086] [INFO] GTDB search result was written to OceanDNA-b40495/result_gtdb.tsv
[2023-03-15 16:49:07,089] [INFO] ===== GTDB Search completed =====
[2023-03-15 16:49:07,093] [INFO] DFAST_QC result json was written to OceanDNA-b40495/dqc_result.json
[2023-03-15 16:49:07,093] [INFO] DFAST_QC completed!
[2023-03-15 16:49:07,093] [INFO] Total running time: 0h1m42s