[2023-03-15 05:00:03,408] [INFO] DFAST_QC pipeline started.
[2023-03-15 05:00:03,410] [INFO] DFAST_QC version: 0.5.7
[2023-03-15 05:00:03,410] [INFO] DQC Reference Directory: /var/lib/cwl/stgf6a58b42-1a04-45a8-8daf-2636381caf03/dqc_reference
[2023-03-15 05:00:04,562] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-15 05:00:04,562] [INFO] Task started: Prodigal
[2023-03-15 05:00:04,563] [INFO] Running command: cat /var/lib/cwl/stgb9f4c8ba-fa5f-4c38-baa4-3390a00b121b/OceanDNA-b40527.fa | prodigal -d OceanDNA-b40527/cds.fna -a OceanDNA-b40527/protein.faa -g 11 -q > /dev/null
[2023-03-15 05:00:10,911] [INFO] Task succeeded: Prodigal
[2023-03-15 05:00:10,911] [INFO] Task started: HMMsearch
[2023-03-15 05:00:10,911] [INFO] Running command: hmmsearch --tblout OceanDNA-b40527/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf6a58b42-1a04-45a8-8daf-2636381caf03/dqc_reference/reference_markers.hmm OceanDNA-b40527/protein.faa > /dev/null
[2023-03-15 05:00:11,055] [INFO] Task succeeded: HMMsearch
[2023-03-15 05:00:11,055] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgb9f4c8ba-fa5f-4c38-baa4-3390a00b121b/OceanDNA-b40527.fa]
[2023-03-15 05:00:11,080] [INFO] Query marker FASTA was written to OceanDNA-b40527/markers.fasta
[2023-03-15 05:00:11,081] [INFO] Task started: Blastn
[2023-03-15 05:00:11,081] [INFO] Running command: blastn -query OceanDNA-b40527/markers.fasta -db /var/lib/cwl/stgf6a58b42-1a04-45a8-8daf-2636381caf03/dqc_reference/reference_markers.fasta -out OceanDNA-b40527/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:00:11,592] [INFO] Task succeeded: Blastn
[2023-03-15 05:00:11,600] [INFO] Selected 22 target genomes.
[2023-03-15 05:00:11,601] [INFO] Target genome list was writen to OceanDNA-b40527/target_genomes.txt
[2023-03-15 05:00:11,614] [INFO] Task started: fastANI
[2023-03-15 05:00:11,614] [INFO] Running command: fastANI --query /var/lib/cwl/stgb9f4c8ba-fa5f-4c38-baa4-3390a00b121b/OceanDNA-b40527.fa --refList OceanDNA-b40527/target_genomes.txt --output OceanDNA-b40527/fastani_result.tsv --threads 1
[2023-03-15 05:00:23,591] [INFO] Task succeeded: fastANI
[2023-03-15 05:00:23,591] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf6a58b42-1a04-45a8-8daf-2636381caf03/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-15 05:00:23,591] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf6a58b42-1a04-45a8-8daf-2636381caf03/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-15 05:00:23,591] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-15 05:00:23,591] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-15 05:00:23,592] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-15 05:00:23,593] [INFO] DFAST Taxonomy check result was written to OceanDNA-b40527/tc_result.tsv
[2023-03-15 05:00:23,596] [INFO] ===== Taxonomy check completed =====
[2023-03-15 05:00:23,596] [INFO] ===== Start completeness check using CheckM =====
[2023-03-15 05:00:23,596] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf6a58b42-1a04-45a8-8daf-2636381caf03/dqc_reference/checkm_data
[2023-03-15 05:00:23,599] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-15 05:00:23,605] [INFO] Task started: CheckM
[2023-03-15 05:00:23,605] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b40527/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b40527/checkm_input OceanDNA-b40527/checkm_result
[2023-03-15 05:00:45,646] [INFO] Task succeeded: CheckM
[2023-03-15 05:00:45,647] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 73.61%
Contamintation: 4.55%
Strain heterogeneity: 50.00%
--------------------------------------------------------------------------------
[2023-03-15 05:00:45,687] [INFO] ===== Completeness check finished =====
[2023-03-15 05:00:45,687] [INFO] ===== Start GTDB Search =====
[2023-03-15 05:00:45,687] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b40527/markers.fasta)
[2023-03-15 05:00:45,688] [INFO] Task started: Blastn
[2023-03-15 05:00:45,688] [INFO] Running command: blastn -query OceanDNA-b40527/markers.fasta -db /var/lib/cwl/stgf6a58b42-1a04-45a8-8daf-2636381caf03/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b40527/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-15 05:00:46,426] [INFO] Task succeeded: Blastn
[2023-03-15 05:00:46,440] [INFO] Selected 7 target genomes.
[2023-03-15 05:00:46,440] [INFO] Target genome list was writen to OceanDNA-b40527/target_genomes_gtdb.txt
[2023-03-15 05:00:46,552] [INFO] Task started: fastANI
[2023-03-15 05:00:46,552] [INFO] Running command: fastANI --query /var/lib/cwl/stgb9f4c8ba-fa5f-4c38-baa4-3390a00b121b/OceanDNA-b40527.fa --refList OceanDNA-b40527/target_genomes_gtdb.txt --output OceanDNA-b40527/fastani_result_gtdb.tsv --threads 1
[2023-03-15 05:00:49,460] [INFO] Task succeeded: fastANI
[2023-03-15 05:00:49,465] [INFO] Found 6 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-15 05:00:49,465] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002691885.1	s__UBA9145 sp002691885	91.4986	198	229	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudohongiellaceae;g__UBA9145	95.0	100.00	100.00	0.99	0.99	2	-
GCA_002729315.1	s__UBA9145 sp002729315	91.2376	204	229	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudohongiellaceae;g__UBA9145	95.0	N/A	N/A	N/A	N/A	1	-
GCA_008081045.1	s__UBA9145 sp008081045	87.6687	200	229	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudohongiellaceae;g__UBA9145	95.0	98.92	98.40	0.89	0.82	4	-
GCA_902597095.1	s__UBA9145 sp902597095	87.4264	166	229	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudohongiellaceae;g__UBA9145	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902537815.1	s__UBA9145 sp902537815	87.006	158	229	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudohongiellaceae;g__UBA9145	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002719575.1	s__UBA9145 sp002719575	79.0579	116	229	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudohongiellaceae;g__UBA9145	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-15 05:00:49,467] [INFO] GTDB search result was written to OceanDNA-b40527/result_gtdb.tsv
[2023-03-15 05:00:49,469] [INFO] ===== GTDB Search completed =====
[2023-03-15 05:00:49,472] [INFO] DFAST_QC result json was written to OceanDNA-b40527/dqc_result.json
[2023-03-15 05:00:49,472] [INFO] DFAST_QC completed!
[2023-03-15 05:00:49,472] [INFO] Total running time: 0h0m46s