[2023-03-18 07:54:29,548] [INFO] DFAST_QC pipeline started.
[2023-03-18 07:54:29,548] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 07:54:29,548] [INFO] DQC Reference Directory: /var/lib/cwl/stg5e95b0f8-6ca0-4720-9ef1-2a3b5302cb1a/dqc_reference
[2023-03-18 07:54:30,667] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 07:54:30,668] [INFO] Task started: Prodigal
[2023-03-18 07:54:30,668] [INFO] Running command: cat /var/lib/cwl/stgaeb9acd9-bb1c-4e1c-8376-a56031e55dc1/OceanDNA-b4157.fa | prodigal -d OceanDNA-b4157/cds.fna -a OceanDNA-b4157/protein.faa -g 11 -q > /dev/null
[2023-03-18 07:54:37,514] [INFO] Task succeeded: Prodigal
[2023-03-18 07:54:37,515] [INFO] Task started: HMMsearch
[2023-03-18 07:54:37,515] [INFO] Running command: hmmsearch --tblout OceanDNA-b4157/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg5e95b0f8-6ca0-4720-9ef1-2a3b5302cb1a/dqc_reference/reference_markers.hmm OceanDNA-b4157/protein.faa > /dev/null
[2023-03-18 07:54:37,663] [INFO] Task succeeded: HMMsearch
[2023-03-18 07:54:37,664] [INFO] Found 6/6 markers.
[2023-03-18 07:54:37,681] [INFO] Query marker FASTA was written to OceanDNA-b4157/markers.fasta
[2023-03-18 07:54:37,682] [INFO] Task started: Blastn
[2023-03-18 07:54:37,682] [INFO] Running command: blastn -query OceanDNA-b4157/markers.fasta -db /var/lib/cwl/stg5e95b0f8-6ca0-4720-9ef1-2a3b5302cb1a/dqc_reference/reference_markers.fasta -out OceanDNA-b4157/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 07:54:38,173] [INFO] Task succeeded: Blastn
[2023-03-18 07:54:38,176] [INFO] Selected 15 target genomes.
[2023-03-18 07:54:38,176] [INFO] Target genome list was writen to OceanDNA-b4157/target_genomes.txt
[2023-03-18 07:54:38,183] [INFO] Task started: fastANI
[2023-03-18 07:54:38,183] [INFO] Running command: fastANI --query /var/lib/cwl/stgaeb9acd9-bb1c-4e1c-8376-a56031e55dc1/OceanDNA-b4157.fa --refList OceanDNA-b4157/target_genomes.txt --output OceanDNA-b4157/fastani_result.tsv --threads 1
[2023-03-18 07:54:47,955] [INFO] Task succeeded: fastANI
[2023-03-18 07:54:47,955] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg5e95b0f8-6ca0-4720-9ef1-2a3b5302cb1a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 07:54:47,956] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg5e95b0f8-6ca0-4720-9ef1-2a3b5302cb1a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 07:54:47,956] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 07:54:47,956] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 07:54:47,956] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 07:54:47,957] [INFO] DFAST Taxonomy check result was written to OceanDNA-b4157/tc_result.tsv
[2023-03-18 07:54:47,957] [INFO] ===== Taxonomy check completed =====
[2023-03-18 07:54:47,958] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 07:54:47,958] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg5e95b0f8-6ca0-4720-9ef1-2a3b5302cb1a/dqc_reference/checkm_data
[2023-03-18 07:54:47,961] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 07:54:47,965] [INFO] Task started: CheckM
[2023-03-18 07:54:47,965] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b4157/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b4157/checkm_input OceanDNA-b4157/checkm_result
[2023-03-18 07:55:11,543] [INFO] Task succeeded: CheckM
[2023-03-18 07:55:11,544] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 70.56%
Contamintation: 0.38%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-18 07:55:11,546] [INFO] ===== Completeness check finished =====
[2023-03-18 07:55:11,546] [INFO] ===== Start GTDB Search =====
[2023-03-18 07:55:11,546] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b4157/markers.fasta)
[2023-03-18 07:55:11,547] [INFO] Task started: Blastn
[2023-03-18 07:55:11,547] [INFO] Running command: blastn -query OceanDNA-b4157/markers.fasta -db /var/lib/cwl/stg5e95b0f8-6ca0-4720-9ef1-2a3b5302cb1a/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b4157/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 07:55:12,176] [INFO] Task succeeded: Blastn
[2023-03-18 07:55:12,177] [INFO] Selected 12 target genomes.
[2023-03-18 07:55:12,177] [INFO] Target genome list was writen to OceanDNA-b4157/target_genomes_gtdb.txt
[2023-03-18 07:55:12,214] [INFO] Task started: fastANI
[2023-03-18 07:55:12,215] [INFO] Running command: fastANI --query /var/lib/cwl/stgaeb9acd9-bb1c-4e1c-8376-a56031e55dc1/OceanDNA-b4157.fa --refList OceanDNA-b4157/target_genomes_gtdb.txt --output OceanDNA-b4157/fastani_result_gtdb.tsv --threads 1
[2023-03-18 07:55:17,544] [INFO] Task succeeded: fastANI
[2023-03-18 07:55:17,549] [INFO] Found 6 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 07:55:17,549] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_902605755.1	s__UBA4465 sp902605755	89.3915	83	135	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002691385.1	s__UBA4465 sp002691385	82.389	107	135	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905479015.1	s__UBA4465 sp002470835	80.7284	115	135	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	99.62	98.40	0.95	0.92	11	-
GCA_905182375.1	s__UBA4465 sp905182375	80.344	105	135	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002292365.1	s__UBA4465 sp002292365	80.0644	99	135	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	99.58	99.58	0.82	0.82	2	-
GCA_905182655.1	s__UBA4465 sp905182655	79.7836	54	135	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 07:55:17,549] [INFO] GTDB search result was written to OceanDNA-b4157/result_gtdb.tsv
[2023-03-18 07:55:17,549] [INFO] ===== GTDB Search completed =====
[2023-03-18 07:55:17,550] [INFO] DFAST_QC result json was written to OceanDNA-b4157/dqc_result.json
[2023-03-18 07:55:17,550] [INFO] DFAST_QC completed!
[2023-03-18 07:55:17,550] [INFO] Total running time: 0h0m48s