[2023-03-16 01:12:29,294] [INFO] DFAST_QC pipeline started.
[2023-03-16 01:12:29,294] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 01:12:29,294] [INFO] DQC Reference Directory: /var/lib/cwl/stgc6621236-85b4-43b6-80ed-130e72f04a2d/dqc_reference
[2023-03-16 01:12:30,447] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 01:12:30,461] [INFO] Task started: Prodigal
[2023-03-16 01:12:30,462] [INFO] Running command: cat /var/lib/cwl/stgc41902db-2682-4eb7-b94a-f4edb370dbc9/OceanDNA-b4159.fa | prodigal -d OceanDNA-b4159/cds.fna -a OceanDNA-b4159/protein.faa -g 11 -q > /dev/null
[2023-03-16 01:12:44,691] [INFO] Task succeeded: Prodigal
[2023-03-16 01:12:44,692] [INFO] Task started: HMMsearch
[2023-03-16 01:12:44,692] [INFO] Running command: hmmsearch --tblout OceanDNA-b4159/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc6621236-85b4-43b6-80ed-130e72f04a2d/dqc_reference/reference_markers.hmm OceanDNA-b4159/protein.faa > /dev/null
[2023-03-16 01:12:44,889] [INFO] Task succeeded: HMMsearch
[2023-03-16 01:12:44,890] [INFO] Found 6/6 markers.
[2023-03-16 01:12:44,907] [INFO] Query marker FASTA was written to OceanDNA-b4159/markers.fasta
[2023-03-16 01:12:44,909] [INFO] Task started: Blastn
[2023-03-16 01:12:44,909] [INFO] Running command: blastn -query OceanDNA-b4159/markers.fasta -db /var/lib/cwl/stgc6621236-85b4-43b6-80ed-130e72f04a2d/dqc_reference/reference_markers.fasta -out OceanDNA-b4159/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 01:12:45,426] [INFO] Task succeeded: Blastn
[2023-03-16 01:12:45,427] [INFO] Selected 16 target genomes.
[2023-03-16 01:12:45,428] [INFO] Target genome list was writen to OceanDNA-b4159/target_genomes.txt
[2023-03-16 01:12:45,462] [INFO] Task started: fastANI
[2023-03-16 01:12:45,462] [INFO] Running command: fastANI --query /var/lib/cwl/stgc41902db-2682-4eb7-b94a-f4edb370dbc9/OceanDNA-b4159.fa --refList OceanDNA-b4159/target_genomes.txt --output OceanDNA-b4159/fastani_result.tsv --threads 1
[2023-03-16 01:12:54,840] [INFO] Task succeeded: fastANI
[2023-03-16 01:12:54,840] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc6621236-85b4-43b6-80ed-130e72f04a2d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 01:12:54,841] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc6621236-85b4-43b6-80ed-130e72f04a2d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 01:12:54,841] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 01:12:54,841] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 01:12:54,841] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 01:12:54,842] [INFO] DFAST Taxonomy check result was written to OceanDNA-b4159/tc_result.tsv
[2023-03-16 01:12:54,845] [INFO] ===== Taxonomy check completed =====
[2023-03-16 01:12:54,845] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 01:12:54,845] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc6621236-85b4-43b6-80ed-130e72f04a2d/dqc_reference/checkm_data
[2023-03-16 01:12:54,848] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 01:12:54,859] [INFO] Task started: CheckM
[2023-03-16 01:12:54,860] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b4159/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b4159/checkm_input OceanDNA-b4159/checkm_result
[2023-03-16 01:13:34,504] [INFO] Task succeeded: CheckM
[2023-03-16 01:13:34,505] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.17%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-16 01:13:34,512] [INFO] ===== Completeness check finished =====
[2023-03-16 01:13:34,512] [INFO] ===== Start GTDB Search =====
[2023-03-16 01:13:34,512] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b4159/markers.fasta)
[2023-03-16 01:13:34,513] [INFO] Task started: Blastn
[2023-03-16 01:13:34,513] [INFO] Running command: blastn -query OceanDNA-b4159/markers.fasta -db /var/lib/cwl/stgc6621236-85b4-43b6-80ed-130e72f04a2d/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b4159/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 01:13:35,217] [INFO] Task succeeded: Blastn
[2023-03-16 01:13:35,219] [INFO] Selected 15 target genomes.
[2023-03-16 01:13:35,219] [INFO] Target genome list was writen to OceanDNA-b4159/target_genomes_gtdb.txt
[2023-03-16 01:13:35,627] [INFO] Task started: fastANI
[2023-03-16 01:13:35,627] [INFO] Running command: fastANI --query /var/lib/cwl/stgc41902db-2682-4eb7-b94a-f4edb370dbc9/OceanDNA-b4159.fa --refList OceanDNA-b4159/target_genomes_gtdb.txt --output OceanDNA-b4159/fastani_result_gtdb.tsv --threads 1
[2023-03-16 01:13:43,051] [INFO] Task succeeded: fastANI
[2023-03-16 01:13:43,056] [INFO] Found 7 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 01:13:43,056] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_902605755.1	s__UBA4465 sp902605755	89.601	359	683	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002691385.1	s__UBA4465 sp002691385	82.0427	507	683	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905182375.1	s__UBA4465 sp905182375	80.2665	453	683	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905479015.1	s__UBA4465 sp002470835	80.2576	484	683	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	99.62	98.40	0.95	0.92	11	-
GCA_002292365.1	s__UBA4465 sp002292365	80.1148	420	683	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	99.58	99.58	0.82	0.82	2	-
GCA_905182655.1	s__UBA4465 sp905182655	79.353	269	683	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013204595.1	s__UBA4465 sp013204595	75.9789	57	683	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Cyclobacteriaceae;g__UBA4465	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-16 01:13:43,057] [INFO] GTDB search result was written to OceanDNA-b4159/result_gtdb.tsv
[2023-03-16 01:13:43,059] [INFO] ===== GTDB Search completed =====
[2023-03-16 01:13:43,060] [INFO] DFAST_QC result json was written to OceanDNA-b4159/dqc_result.json
[2023-03-16 01:13:43,060] [INFO] DFAST_QC completed!
[2023-03-16 01:13:43,060] [INFO] Total running time: 0h1m14s