[2023-03-18 04:16:53,177] [INFO] DFAST_QC pipeline started.
[2023-03-18 04:16:53,178] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 04:16:53,178] [INFO] DQC Reference Directory: /var/lib/cwl/stgdebbbbdf-8296-4d95-87bc-10d8159fa7e8/dqc_reference
[2023-03-18 04:16:54,279] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 04:16:54,279] [INFO] Task started: Prodigal
[2023-03-18 04:16:54,279] [INFO] Running command: cat /var/lib/cwl/stg6a9a39d9-6719-4fde-bbbc-2dd99a4b8000/OceanDNA-b42306.fa | prodigal -d OceanDNA-b42306/cds.fna -a OceanDNA-b42306/protein.faa -g 11 -q > /dev/null
[2023-03-18 04:16:56,251] [INFO] Task succeeded: Prodigal
[2023-03-18 04:16:56,252] [INFO] Task started: HMMsearch
[2023-03-18 04:16:56,252] [INFO] Running command: hmmsearch --tblout OceanDNA-b42306/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgdebbbbdf-8296-4d95-87bc-10d8159fa7e8/dqc_reference/reference_markers.hmm OceanDNA-b42306/protein.faa > /dev/null
[2023-03-18 04:16:56,414] [INFO] Task succeeded: HMMsearch
[2023-03-18 04:16:56,414] [WARNING] Found 5/6 markers. [/var/lib/cwl/stg6a9a39d9-6719-4fde-bbbc-2dd99a4b8000/OceanDNA-b42306.fa]
[2023-03-18 04:16:56,419] [INFO] Query marker FASTA was written to OceanDNA-b42306/markers.fasta
[2023-03-18 04:16:56,421] [INFO] Task started: Blastn
[2023-03-18 04:16:56,421] [INFO] Running command: blastn -query OceanDNA-b42306/markers.fasta -db /var/lib/cwl/stgdebbbbdf-8296-4d95-87bc-10d8159fa7e8/dqc_reference/reference_markers.fasta -out OceanDNA-b42306/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 04:16:57,087] [INFO] Task succeeded: Blastn
[2023-03-18 04:16:57,088] [INFO] Selected 10 target genomes.
[2023-03-18 04:16:57,088] [INFO] Target genome list was writen to OceanDNA-b42306/target_genomes.txt
[2023-03-18 04:16:57,104] [INFO] Task started: fastANI
[2023-03-18 04:16:57,104] [INFO] Running command: fastANI --query /var/lib/cwl/stg6a9a39d9-6719-4fde-bbbc-2dd99a4b8000/OceanDNA-b42306.fa --refList OceanDNA-b42306/target_genomes.txt --output OceanDNA-b42306/fastani_result.tsv --threads 1
[2023-03-18 04:17:00,813] [INFO] Task succeeded: fastANI
[2023-03-18 04:17:00,813] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgdebbbbdf-8296-4d95-87bc-10d8159fa7e8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 04:17:00,813] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgdebbbbdf-8296-4d95-87bc-10d8159fa7e8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 04:17:00,813] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 04:17:00,813] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 04:17:00,814] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 04:17:00,814] [INFO] DFAST Taxonomy check result was written to OceanDNA-b42306/tc_result.tsv
[2023-03-18 04:17:00,814] [INFO] ===== Taxonomy check completed =====
[2023-03-18 04:17:00,814] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 04:17:00,814] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgdebbbbdf-8296-4d95-87bc-10d8159fa7e8/dqc_reference/checkm_data
[2023-03-18 04:17:00,868] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 04:17:01,151] [INFO] Task started: CheckM
[2023-03-18 04:17:01,151] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b42306/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b42306/checkm_input OceanDNA-b42306/checkm_result
[2023-03-18 04:17:16,929] [INFO] Task succeeded: CheckM
[2023-03-18 04:17:16,930] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 27.08%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 04:17:16,931] [INFO] ===== Completeness check finished =====
[2023-03-18 04:17:16,931] [INFO] ===== Start GTDB Search =====
[2023-03-18 04:17:16,931] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b42306/markers.fasta)
[2023-03-18 04:17:16,933] [INFO] Task started: Blastn
[2023-03-18 04:17:16,933] [INFO] Running command: blastn -query OceanDNA-b42306/markers.fasta -db /var/lib/cwl/stgdebbbbdf-8296-4d95-87bc-10d8159fa7e8/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b42306/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 04:17:17,984] [INFO] Task succeeded: Blastn
[2023-03-18 04:17:17,985] [INFO] Selected 21 target genomes.
[2023-03-18 04:17:17,985] [INFO] Target genome list was writen to OceanDNA-b42306/target_genomes_gtdb.txt
[2023-03-18 04:17:18,052] [INFO] Task started: fastANI
[2023-03-18 04:17:18,052] [INFO] Running command: fastANI --query /var/lib/cwl/stg6a9a39d9-6719-4fde-bbbc-2dd99a4b8000/OceanDNA-b42306.fa --refList OceanDNA-b42306/target_genomes_gtdb.txt --output OceanDNA-b42306/fastani_result_gtdb.tsv --threads 1
[2023-03-18 04:17:21,559] [INFO] Task succeeded: fastANI
[2023-03-18 04:17:21,561] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-18 04:17:21,561] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002721385.1	s__SAR86A sp002721385	98.498	74	102	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SAR86A	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2023-03-18 04:17:21,561] [INFO] GTDB search result was written to OceanDNA-b42306/result_gtdb.tsv
[2023-03-18 04:17:21,561] [INFO] ===== GTDB Search completed =====
[2023-03-18 04:17:21,562] [INFO] DFAST_QC result json was written to OceanDNA-b42306/dqc_result.json
[2023-03-18 04:17:21,562] [INFO] DFAST_QC completed!
[2023-03-18 04:17:21,562] [INFO] Total running time: 0h0m28s