[2023-03-18 04:54:25,331] [INFO] DFAST_QC pipeline started.
[2023-03-18 04:54:25,331] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 04:54:25,331] [INFO] DQC Reference Directory: /var/lib/cwl/stg638ec960-6d59-4e5c-888a-e0b701ec5c02/dqc_reference
[2023-03-18 04:54:26,988] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 04:54:26,989] [INFO] Task started: Prodigal
[2023-03-18 04:54:26,989] [INFO] Running command: cat /var/lib/cwl/stg6b458de0-568e-4300-8c3f-e2ae779f79cd/OceanDNA-b42318.fa | prodigal -d OceanDNA-b42318/cds.fna -a OceanDNA-b42318/protein.faa -g 11 -q > /dev/null
[2023-03-18 04:54:30,413] [INFO] Task succeeded: Prodigal
[2023-03-18 04:54:30,414] [INFO] Task started: HMMsearch
[2023-03-18 04:54:30,414] [INFO] Running command: hmmsearch --tblout OceanDNA-b42318/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg638ec960-6d59-4e5c-888a-e0b701ec5c02/dqc_reference/reference_markers.hmm OceanDNA-b42318/protein.faa > /dev/null
[2023-03-18 04:54:30,568] [INFO] Task succeeded: HMMsearch
[2023-03-18 04:54:30,569] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg6b458de0-568e-4300-8c3f-e2ae779f79cd/OceanDNA-b42318.fa]
[2023-03-18 04:54:30,576] [INFO] Query marker FASTA was written to OceanDNA-b42318/markers.fasta
[2023-03-18 04:54:30,578] [INFO] Task started: Blastn
[2023-03-18 04:54:30,578] [INFO] Running command: blastn -query OceanDNA-b42318/markers.fasta -db /var/lib/cwl/stg638ec960-6d59-4e5c-888a-e0b701ec5c02/dqc_reference/reference_markers.fasta -out OceanDNA-b42318/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 04:54:34,137] [INFO] Task succeeded: Blastn
[2023-03-18 04:54:34,138] [INFO] Selected 18 target genomes.
[2023-03-18 04:54:34,138] [INFO] Target genome list was writen to OceanDNA-b42318/target_genomes.txt
[2023-03-18 04:54:34,155] [INFO] Task started: fastANI
[2023-03-18 04:54:34,155] [INFO] Running command: fastANI --query /var/lib/cwl/stg6b458de0-568e-4300-8c3f-e2ae779f79cd/OceanDNA-b42318.fa --refList OceanDNA-b42318/target_genomes.txt --output OceanDNA-b42318/fastani_result.tsv --threads 1
[2023-03-18 04:54:41,388] [INFO] Task succeeded: fastANI
[2023-03-18 04:54:41,389] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg638ec960-6d59-4e5c-888a-e0b701ec5c02/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 04:54:41,389] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg638ec960-6d59-4e5c-888a-e0b701ec5c02/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 04:54:41,389] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 04:54:41,389] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 04:54:41,389] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 04:54:41,389] [INFO] DFAST Taxonomy check result was written to OceanDNA-b42318/tc_result.tsv
[2023-03-18 04:54:41,390] [INFO] ===== Taxonomy check completed =====
[2023-03-18 04:54:41,390] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 04:54:41,390] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg638ec960-6d59-4e5c-888a-e0b701ec5c02/dqc_reference/checkm_data
[2023-03-18 04:54:41,393] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 04:54:41,396] [INFO] Task started: CheckM
[2023-03-18 04:54:41,397] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b42318/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b42318/checkm_input OceanDNA-b42318/checkm_result
[2023-03-18 04:54:59,668] [INFO] Task succeeded: CheckM
[2023-03-18 04:54:59,669] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 39.58%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 04:54:59,677] [INFO] ===== Completeness check finished =====
[2023-03-18 04:54:59,677] [INFO] ===== Start GTDB Search =====
[2023-03-18 04:54:59,678] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b42318/markers.fasta)
[2023-03-18 04:54:59,680] [INFO] Task started: Blastn
[2023-03-18 04:54:59,680] [INFO] Running command: blastn -query OceanDNA-b42318/markers.fasta -db /var/lib/cwl/stg638ec960-6d59-4e5c-888a-e0b701ec5c02/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b42318/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 04:55:00,595] [INFO] Task succeeded: Blastn
[2023-03-18 04:55:00,596] [INFO] Selected 17 target genomes.
[2023-03-18 04:55:00,596] [INFO] Target genome list was writen to OceanDNA-b42318/target_genomes_gtdb.txt
[2023-03-18 04:55:00,630] [INFO] Task started: fastANI
[2023-03-18 04:55:00,630] [INFO] Running command: fastANI --query /var/lib/cwl/stg6b458de0-568e-4300-8c3f-e2ae779f79cd/OceanDNA-b42318.fa --refList OceanDNA-b42318/target_genomes_gtdb.txt --output OceanDNA-b42318/fastani_result_gtdb.tsv --threads 1
[2023-03-18 04:55:05,037] [INFO] Task succeeded: fastANI
[2023-03-18 04:55:05,044] [INFO] Found 11 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 04:55:05,044] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_902515155.1	s__SCGC-AAA076-P13 sp902515155	78.7195	103	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905182885.1	s__SCGC-AAA076-P13 sp905182885	78.3526	88	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	N/A	N/A	N/A	N/A	1	-
GCA_905478035.1	s__SCGC-AAA076-P13 sp905478035	77.8177	104	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902558375.1	s__SCGC-AAA076-P13 sp902558375	77.7164	52	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	N/A	N/A	N/A	N/A	1	-
GCA_000384935.1	s__SCGC-AAA076-P13 sp000384935	77.6976	104	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018607365.1	s__SCGC-AAA076-P13 sp018607365	77.6268	109	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	N/A	N/A	N/A	N/A	1	-
GCA_000385055.1	s__SCGC-AAA076-P13 sp000385055	77.5611	83	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	98.19	98.19	0.89	0.89	2	-
GCA_902591665.1	s__SCGC-AAA076-P13 sp902591665	77.3347	72	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	99.81	99.81	0.85	0.85	2	-
GCA_902635645.1	s__SCGC-AAA076-P13 sp902635645	77.2115	60	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	97.13	97.13	0.67	0.67	2	-
GCA_010024215.1	s__SCGC-AAA076-P13 sp010024215	77.0897	63	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	98.04	96.58	0.74	0.68	5	-
GCA_902591565.1	s__SCGC-AAA076-P13 sp902591565	76.8133	69	218	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__D2472;g__SCGC-AAA076-P13	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 04:55:05,045] [INFO] GTDB search result was written to OceanDNA-b42318/result_gtdb.tsv
[2023-03-18 04:55:05,045] [INFO] ===== GTDB Search completed =====
[2023-03-18 04:55:05,046] [INFO] DFAST_QC result json was written to OceanDNA-b42318/dqc_result.json
[2023-03-18 04:55:05,046] [INFO] DFAST_QC completed!
[2023-03-18 04:55:05,046] [INFO] Total running time: 0h0m40s