[2023-03-16 02:38:04,621] [INFO] DFAST_QC pipeline started.
[2023-03-16 02:38:04,621] [INFO] DFAST_QC version: 0.5.7
[2023-03-16 02:38:04,621] [INFO] DQC Reference Directory: /var/lib/cwl/stga01a8ca6-33c1-4410-a48a-f4814d48341f/dqc_reference
[2023-03-16 02:38:05,900] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-16 02:38:05,901] [INFO] Task started: Prodigal
[2023-03-16 02:38:05,901] [INFO] Running command: cat /var/lib/cwl/stgd3205620-74a8-4797-ad20-721cbdb5ff5a/OceanDNA-b42499.fa | prodigal -d OceanDNA-b42499/cds.fna -a OceanDNA-b42499/protein.faa -g 11 -q > /dev/null
[2023-03-16 02:38:10,127] [INFO] Task succeeded: Prodigal
[2023-03-16 02:38:10,127] [INFO] Task started: HMMsearch
[2023-03-16 02:38:10,127] [INFO] Running command: hmmsearch --tblout OceanDNA-b42499/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga01a8ca6-33c1-4410-a48a-f4814d48341f/dqc_reference/reference_markers.hmm OceanDNA-b42499/protein.faa > /dev/null
[2023-03-16 02:38:10,290] [INFO] Task succeeded: HMMsearch
[2023-03-16 02:38:10,290] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgd3205620-74a8-4797-ad20-721cbdb5ff5a/OceanDNA-b42499.fa]
[2023-03-16 02:38:10,297] [INFO] Query marker FASTA was written to OceanDNA-b42499/markers.fasta
[2023-03-16 02:38:10,299] [INFO] Task started: Blastn
[2023-03-16 02:38:10,299] [INFO] Running command: blastn -query OceanDNA-b42499/markers.fasta -db /var/lib/cwl/stga01a8ca6-33c1-4410-a48a-f4814d48341f/dqc_reference/reference_markers.fasta -out OceanDNA-b42499/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 02:38:11,080] [INFO] Task succeeded: Blastn
[2023-03-16 02:38:11,081] [INFO] Selected 14 target genomes.
[2023-03-16 02:38:11,081] [INFO] Target genome list was writen to OceanDNA-b42499/target_genomes.txt
[2023-03-16 02:38:11,089] [INFO] Task started: fastANI
[2023-03-16 02:38:11,089] [INFO] Running command: fastANI --query /var/lib/cwl/stgd3205620-74a8-4797-ad20-721cbdb5ff5a/OceanDNA-b42499.fa --refList OceanDNA-b42499/target_genomes.txt --output OceanDNA-b42499/fastani_result.tsv --threads 1
[2023-03-16 02:38:18,590] [INFO] Task succeeded: fastANI
[2023-03-16 02:38:18,590] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga01a8ca6-33c1-4410-a48a-f4814d48341f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-16 02:38:18,590] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga01a8ca6-33c1-4410-a48a-f4814d48341f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-16 02:38:18,590] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-16 02:38:18,590] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-16 02:38:18,590] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-16 02:38:18,591] [INFO] DFAST Taxonomy check result was written to OceanDNA-b42499/tc_result.tsv
[2023-03-16 02:38:18,591] [INFO] ===== Taxonomy check completed =====
[2023-03-16 02:38:18,591] [INFO] ===== Start completeness check using CheckM =====
[2023-03-16 02:38:18,591] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga01a8ca6-33c1-4410-a48a-f4814d48341f/dqc_reference/checkm_data
[2023-03-16 02:38:18,593] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-16 02:38:18,595] [INFO] Task started: CheckM
[2023-03-16 02:38:18,595] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b42499/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b42499/checkm_input OceanDNA-b42499/checkm_result
[2023-03-16 02:38:39,203] [INFO] Task succeeded: CheckM
[2023-03-16 02:38:39,204] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 83.33%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-16 02:38:39,205] [INFO] ===== Completeness check finished =====
[2023-03-16 02:38:39,205] [INFO] ===== Start GTDB Search =====
[2023-03-16 02:38:39,205] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b42499/markers.fasta)
[2023-03-16 02:38:39,209] [INFO] Task started: Blastn
[2023-03-16 02:38:39,209] [INFO] Running command: blastn -query OceanDNA-b42499/markers.fasta -db /var/lib/cwl/stga01a8ca6-33c1-4410-a48a-f4814d48341f/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b42499/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-16 02:38:40,559] [INFO] Task succeeded: Blastn
[2023-03-16 02:38:40,560] [INFO] Selected 18 target genomes.
[2023-03-16 02:38:40,560] [INFO] Target genome list was writen to OceanDNA-b42499/target_genomes_gtdb.txt
[2023-03-16 02:38:40,929] [INFO] Task started: fastANI
[2023-03-16 02:38:40,929] [INFO] Running command: fastANI --query /var/lib/cwl/stgd3205620-74a8-4797-ad20-721cbdb5ff5a/OceanDNA-b42499.fa --refList OceanDNA-b42499/target_genomes_gtdb.txt --output OceanDNA-b42499/fastani_result_gtdb.tsv --threads 1
[2023-03-16 02:38:44,873] [INFO] Task succeeded: fastANI
[2023-03-16 02:38:44,876] [INFO] Found 2 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-16 02:38:44,876] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_012965075.1	s__AEGEAN-183 sp012965075	78.281	91	276	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__SAR86;g__AEGEAN-183	95.0	98.73	98.73	0.80	0.80	2	-
GCA_001628005.1	s__AEGEAN-183 sp001628005	77.0899	78	276	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__SAR86;g__AEGEAN-183	95.0	99.32	99.31	0.88	0.87	3	-
--------------------------------------------------------------------------------
[2023-03-16 02:38:44,876] [INFO] GTDB search result was written to OceanDNA-b42499/result_gtdb.tsv
[2023-03-16 02:38:44,876] [INFO] ===== GTDB Search completed =====
[2023-03-16 02:38:44,876] [INFO] DFAST_QC result json was written to OceanDNA-b42499/dqc_result.json
[2023-03-16 02:38:44,877] [INFO] DFAST_QC completed!
[2023-03-16 02:38:44,877] [INFO] Total running time: 0h0m40s