[2023-03-17 07:28:30,375] [INFO] DFAST_QC pipeline started.
[2023-03-17 07:28:30,375] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 07:28:30,375] [INFO] DQC Reference Directory: /var/lib/cwl/stg73a18032-98c2-421b-b788-fe43633e31cb/dqc_reference
[2023-03-17 07:28:31,523] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 07:28:31,524] [INFO] Task started: Prodigal
[2023-03-17 07:28:31,524] [INFO] Running command: cat /var/lib/cwl/stg0009f59b-e004-4fc2-bc99-0a583eb31000/OceanDNA-b42620.fa | prodigal -d OceanDNA-b42620/cds.fna -a OceanDNA-b42620/protein.faa -g 11 -q > /dev/null
[2023-03-17 07:28:36,725] [INFO] Task succeeded: Prodigal
[2023-03-17 07:28:36,725] [INFO] Task started: HMMsearch
[2023-03-17 07:28:36,725] [INFO] Running command: hmmsearch --tblout OceanDNA-b42620/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg73a18032-98c2-421b-b788-fe43633e31cb/dqc_reference/reference_markers.hmm OceanDNA-b42620/protein.faa > /dev/null
[2023-03-17 07:28:36,885] [INFO] Task succeeded: HMMsearch
[2023-03-17 07:28:36,885] [INFO] Found 6/6 markers.
[2023-03-17 07:28:36,896] [INFO] Query marker FASTA was written to OceanDNA-b42620/markers.fasta
[2023-03-17 07:28:36,896] [INFO] Task started: Blastn
[2023-03-17 07:28:36,896] [INFO] Running command: blastn -query OceanDNA-b42620/markers.fasta -db /var/lib/cwl/stg73a18032-98c2-421b-b788-fe43633e31cb/dqc_reference/reference_markers.fasta -out OceanDNA-b42620/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 07:28:37,427] [INFO] Task succeeded: Blastn
[2023-03-17 07:28:37,428] [INFO] Selected 19 target genomes.
[2023-03-17 07:28:37,428] [INFO] Target genome list was writen to OceanDNA-b42620/target_genomes.txt
[2023-03-17 07:28:37,437] [INFO] Task started: fastANI
[2023-03-17 07:28:37,437] [INFO] Running command: fastANI --query /var/lib/cwl/stg0009f59b-e004-4fc2-bc99-0a583eb31000/OceanDNA-b42620.fa --refList OceanDNA-b42620/target_genomes.txt --output OceanDNA-b42620/fastani_result.tsv --threads 1
[2023-03-17 07:28:46,115] [INFO] Task succeeded: fastANI
[2023-03-17 07:28:46,115] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg73a18032-98c2-421b-b788-fe43633e31cb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 07:28:46,116] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg73a18032-98c2-421b-b788-fe43633e31cb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 07:28:46,116] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 07:28:46,116] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 07:28:46,116] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 07:28:46,116] [INFO] DFAST Taxonomy check result was written to OceanDNA-b42620/tc_result.tsv
[2023-03-17 07:28:46,116] [INFO] ===== Taxonomy check completed =====
[2023-03-17 07:28:46,116] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 07:28:46,116] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg73a18032-98c2-421b-b788-fe43633e31cb/dqc_reference/checkm_data
[2023-03-17 07:28:46,119] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 07:28:46,121] [INFO] Task started: CheckM
[2023-03-17 07:28:46,121] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b42620/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b42620/checkm_input OceanDNA-b42620/checkm_result
[2023-03-17 07:29:04,759] [INFO] Task succeeded: CheckM
[2023-03-17 07:29:04,759] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 85.42%
Contamintation: 0.38%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-17 07:29:04,761] [INFO] ===== Completeness check finished =====
[2023-03-17 07:29:04,761] [INFO] ===== Start GTDB Search =====
[2023-03-17 07:29:04,761] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b42620/markers.fasta)
[2023-03-17 07:29:04,761] [INFO] Task started: Blastn
[2023-03-17 07:29:04,761] [INFO] Running command: blastn -query OceanDNA-b42620/markers.fasta -db /var/lib/cwl/stg73a18032-98c2-421b-b788-fe43633e31cb/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b42620/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 07:29:05,599] [INFO] Task succeeded: Blastn
[2023-03-17 07:29:05,600] [INFO] Selected 26 target genomes.
[2023-03-17 07:29:05,600] [INFO] Target genome list was writen to OceanDNA-b42620/target_genomes_gtdb.txt
[2023-03-17 07:29:06,087] [INFO] Task started: fastANI
[2023-03-17 07:29:06,087] [INFO] Running command: fastANI --query /var/lib/cwl/stg0009f59b-e004-4fc2-bc99-0a583eb31000/OceanDNA-b42620.fa --refList OceanDNA-b42620/target_genomes_gtdb.txt --output OceanDNA-b42620/fastani_result_gtdb.tsv --threads 1
[2023-03-17 07:29:13,653] [INFO] Task succeeded: fastANI
[2023-03-17 07:29:13,657] [INFO] Found 6 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-17 07:29:13,657] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_902532015.1	s__GCA-2730855 sp902532015	84.3544	186	325	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__SAR86;g__GCA-2730855	95.0	97.85	97.68	0.69	0.68	3	-
GCA_902532675.1	s__GCA-2730855 sp902532675	81.1739	198	325	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__SAR86;g__GCA-2730855	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902514295.1	s__GCA-2730855 sp902514295	80.0951	182	325	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__SAR86;g__GCA-2730855	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902554355.1	s__GCA-2730855 sp902554355	79.7561	161	325	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__SAR86;g__GCA-2730855	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902604855.1	s__GCA-2707915 sp902604855	76.8346	60	325	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__SAR86;g__GCA-2707915	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902606175.1	s__GCA-2707915 sp902606175	76.6191	54	325	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__SAR86;f__SAR86;g__GCA-2707915	95.0	95.24	95.17	0.72	0.71	3	-
--------------------------------------------------------------------------------
[2023-03-17 07:29:13,658] [INFO] GTDB search result was written to OceanDNA-b42620/result_gtdb.tsv
[2023-03-17 07:29:13,658] [INFO] ===== GTDB Search completed =====
[2023-03-17 07:29:13,658] [INFO] DFAST_QC result json was written to OceanDNA-b42620/dqc_result.json
[2023-03-17 07:29:13,658] [INFO] DFAST_QC completed!
[2023-03-17 07:29:13,658] [INFO] Total running time: 0h0m43s