[2023-03-19 01:13:44,405] [INFO] DFAST_QC pipeline started.
[2023-03-19 01:13:44,405] [INFO] DFAST_QC version: 0.5.7
[2023-03-19 01:13:44,405] [INFO] DQC Reference Directory: /var/lib/cwl/stgab49d25e-064e-4987-9123-160cdec5f84b/dqc_reference
[2023-03-19 01:13:45,504] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-19 01:13:45,504] [INFO] Task started: Prodigal
[2023-03-19 01:13:45,505] [INFO] Running command: cat /var/lib/cwl/stgfbcea397-4501-455b-8baa-10cb0a9d836e/OceanDNA-b43371.fa | prodigal -d OceanDNA-b43371/cds.fna -a OceanDNA-b43371/protein.faa -g 11 -q > /dev/null
[2023-03-19 01:13:51,224] [INFO] Task succeeded: Prodigal
[2023-03-19 01:13:51,224] [INFO] Task started: HMMsearch
[2023-03-19 01:13:51,224] [INFO] Running command: hmmsearch --tblout OceanDNA-b43371/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgab49d25e-064e-4987-9123-160cdec5f84b/dqc_reference/reference_markers.hmm OceanDNA-b43371/protein.faa > /dev/null
[2023-03-19 01:13:51,384] [INFO] Task succeeded: HMMsearch
[2023-03-19 01:13:51,384] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgfbcea397-4501-455b-8baa-10cb0a9d836e/OceanDNA-b43371.fa]
[2023-03-19 01:13:51,394] [INFO] Query marker FASTA was written to OceanDNA-b43371/markers.fasta
[2023-03-19 01:13:51,395] [INFO] Task started: Blastn
[2023-03-19 01:13:51,395] [INFO] Running command: blastn -query OceanDNA-b43371/markers.fasta -db /var/lib/cwl/stgab49d25e-064e-4987-9123-160cdec5f84b/dqc_reference/reference_markers.fasta -out OceanDNA-b43371/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 01:13:51,936] [INFO] Task succeeded: Blastn
[2023-03-19 01:13:51,937] [INFO] Selected 26 target genomes.
[2023-03-19 01:13:51,937] [INFO] Target genome list was writen to OceanDNA-b43371/target_genomes.txt
[2023-03-19 01:13:51,953] [INFO] Task started: fastANI
[2023-03-19 01:13:51,953] [INFO] Running command: fastANI --query /var/lib/cwl/stgfbcea397-4501-455b-8baa-10cb0a9d836e/OceanDNA-b43371.fa --refList OceanDNA-b43371/target_genomes.txt --output OceanDNA-b43371/fastani_result.tsv --threads 1
[2023-03-19 01:14:03,180] [INFO] Task succeeded: fastANI
[2023-03-19 01:14:03,181] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgab49d25e-064e-4987-9123-160cdec5f84b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-19 01:14:03,181] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgab49d25e-064e-4987-9123-160cdec5f84b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-19 01:14:03,184] [INFO] Found 1 fastANI hits (0 hits with ANI > threshold)
[2023-03-19 01:14:03,184] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-19 01:14:03,184] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Candidatus Thioglobus autotrophicus	strain=EF1	GCA_001293165.1	1705394	1705394	type	True	83.275	286	341	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-19 01:14:03,184] [INFO] DFAST Taxonomy check result was written to OceanDNA-b43371/tc_result.tsv
[2023-03-19 01:14:03,184] [INFO] ===== Taxonomy check completed =====
[2023-03-19 01:14:03,184] [INFO] ===== Start completeness check using CheckM =====
[2023-03-19 01:14:03,184] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgab49d25e-064e-4987-9123-160cdec5f84b/dqc_reference/checkm_data
[2023-03-19 01:14:03,185] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-19 01:14:03,220] [INFO] Task started: CheckM
[2023-03-19 01:14:03,221] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b43371/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b43371/checkm_input OceanDNA-b43371/checkm_result
[2023-03-19 01:14:23,556] [INFO] Task succeeded: CheckM
[2023-03-19 01:14:23,556] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 1.36%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-19 01:14:23,558] [INFO] ===== Completeness check finished =====
[2023-03-19 01:14:23,558] [INFO] ===== Start GTDB Search =====
[2023-03-19 01:14:23,559] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b43371/markers.fasta)
[2023-03-19 01:14:23,560] [INFO] Task started: Blastn
[2023-03-19 01:14:23,560] [INFO] Running command: blastn -query OceanDNA-b43371/markers.fasta -db /var/lib/cwl/stgab49d25e-064e-4987-9123-160cdec5f84b/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b43371/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-19 01:14:24,406] [INFO] Task succeeded: Blastn
[2023-03-19 01:14:24,407] [INFO] Selected 15 target genomes.
[2023-03-19 01:14:24,407] [INFO] Target genome list was writen to OceanDNA-b43371/target_genomes_gtdb.txt
[2023-03-19 01:14:24,445] [INFO] Task started: fastANI
[2023-03-19 01:14:24,445] [INFO] Running command: fastANI --query /var/lib/cwl/stgfbcea397-4501-455b-8baa-10cb0a9d836e/OceanDNA-b43371.fa --refList OceanDNA-b43371/target_genomes_gtdb.txt --output OceanDNA-b43371/fastani_result_gtdb.tsv --threads 1
[2023-03-19 01:14:28,825] [INFO] Task succeeded: fastANI
[2023-03-19 01:14:28,834] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-19 01:14:28,834] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018644675.1	s__Thioglobus sp018644675	99.6455	319	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thioglobus	95.0	99.74	99.56	0.96	0.91	16	conclusive
GCF_001293165.1	s__Thioglobus autotrophicus	83.275	286	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thioglobus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_014384345.1	s__Thioglobus pontius	79.8271	220	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thioglobus	95.0	99.87	99.85	0.90	0.85	3	-
GCA_900180385.1	s__Thioglobus sp900180385	79.4651	218	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thioglobus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900128515.1	s__Thioglobus sp900128515	79.4592	200	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thioglobus	95.0	99.64	99.26	0.90	0.80	8	-
GCA_012963715.1	s__Thioglobus sp012963715	78.9923	165	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thioglobus	95.0	97.05	95.18	0.84	0.80	5	-
GCA_002909145.1	s__Thioglobus_B perditus	78.7658	138	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thioglobus_B	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016845205.1	s__UBA2013 sp016845205	78.1513	126	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__UBA2013	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018648985.1	s__UBA2013 sp018648985	77.8965	112	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__UBA2013	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016592615.1	s__Ruthia sp016592615	77.294	106	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Ruthia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900128535.1	s__Thiodubiliella sp900128535	77.161	70	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thiodubiliella	95.0	98.14	96.23	0.87	0.79	15	-
GCF_001298715.1	s__Thiodubiliella sp001298715	77.039	76	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thiodubiliella	95.0	98.96	97.97	0.90	0.82	38	-
GCF_900128405.1	s__Thiodubiliella sp900128405	76.8628	75	341	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__PS1;f__Thioglobaceae;g__Thiodubiliella	95.0	98.90	98.69	0.84	0.78	38	-
--------------------------------------------------------------------------------
[2023-03-19 01:14:28,834] [INFO] GTDB search result was written to OceanDNA-b43371/result_gtdb.tsv
[2023-03-19 01:14:28,834] [INFO] ===== GTDB Search completed =====
[2023-03-19 01:14:28,835] [INFO] DFAST_QC result json was written to OceanDNA-b43371/dqc_result.json
[2023-03-19 01:14:28,836] [INFO] DFAST_QC completed!
[2023-03-19 01:14:28,836] [INFO] Total running time: 0h0m44s