[2023-03-18 01:14:00,955] [INFO] DFAST_QC pipeline started.
[2023-03-18 01:14:00,955] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 01:14:00,955] [INFO] DQC Reference Directory: /var/lib/cwl/stg0faa6360-76d3-4dc5-b364-fc1aa0a46302/dqc_reference
[2023-03-18 01:14:02,090] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 01:14:02,090] [INFO] Task started: Prodigal
[2023-03-18 01:14:02,090] [INFO] Running command: cat /var/lib/cwl/stg6ca9acea-c106-4f70-ab38-5880f23705f0/OceanDNA-b43875.fa | prodigal -d OceanDNA-b43875/cds.fna -a OceanDNA-b43875/protein.faa -g 11 -q > /dev/null
[2023-03-18 01:14:09,689] [INFO] Task succeeded: Prodigal
[2023-03-18 01:14:09,689] [INFO] Task started: HMMsearch
[2023-03-18 01:14:09,689] [INFO] Running command: hmmsearch --tblout OceanDNA-b43875/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg0faa6360-76d3-4dc5-b364-fc1aa0a46302/dqc_reference/reference_markers.hmm OceanDNA-b43875/protein.faa > /dev/null
[2023-03-18 01:14:09,845] [INFO] Task succeeded: HMMsearch
[2023-03-18 01:14:09,846] [INFO] Found 6/6 markers.
[2023-03-18 01:14:09,860] [INFO] Query marker FASTA was written to OceanDNA-b43875/markers.fasta
[2023-03-18 01:14:09,866] [INFO] Task started: Blastn
[2023-03-18 01:14:09,866] [INFO] Running command: blastn -query OceanDNA-b43875/markers.fasta -db /var/lib/cwl/stg0faa6360-76d3-4dc5-b364-fc1aa0a46302/dqc_reference/reference_markers.fasta -out OceanDNA-b43875/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 01:14:10,443] [INFO] Task succeeded: Blastn
[2023-03-18 01:14:10,444] [INFO] Selected 35 target genomes.
[2023-03-18 01:14:10,444] [INFO] Target genome list was writen to OceanDNA-b43875/target_genomes.txt
[2023-03-18 01:14:10,469] [INFO] Task started: fastANI
[2023-03-18 01:14:10,469] [INFO] Running command: fastANI --query /var/lib/cwl/stg6ca9acea-c106-4f70-ab38-5880f23705f0/OceanDNA-b43875.fa --refList OceanDNA-b43875/target_genomes.txt --output OceanDNA-b43875/fastani_result.tsv --threads 1
[2023-03-18 01:14:31,086] [INFO] Task succeeded: fastANI
[2023-03-18 01:14:31,086] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg0faa6360-76d3-4dc5-b364-fc1aa0a46302/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 01:14:31,087] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg0faa6360-76d3-4dc5-b364-fc1aa0a46302/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 01:14:31,087] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 01:14:31,087] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-18 01:14:31,087] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-18 01:14:31,087] [INFO] DFAST Taxonomy check result was written to OceanDNA-b43875/tc_result.tsv
[2023-03-18 01:14:31,087] [INFO] ===== Taxonomy check completed =====
[2023-03-18 01:14:31,087] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 01:14:31,087] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg0faa6360-76d3-4dc5-b364-fc1aa0a46302/dqc_reference/checkm_data
[2023-03-18 01:14:31,090] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 01:14:31,096] [INFO] Task started: CheckM
[2023-03-18 01:14:31,096] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b43875/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b43875/checkm_input OceanDNA-b43875/checkm_result
[2023-03-18 01:14:55,649] [INFO] Task succeeded: CheckM
[2023-03-18 01:14:55,649] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 85.73%
Contamintation: 0.38%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-18 01:14:55,651] [INFO] ===== Completeness check finished =====
[2023-03-18 01:14:55,651] [INFO] ===== Start GTDB Search =====
[2023-03-18 01:14:55,651] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b43875/markers.fasta)
[2023-03-18 01:14:55,652] [INFO] Task started: Blastn
[2023-03-18 01:14:55,652] [INFO] Running command: blastn -query OceanDNA-b43875/markers.fasta -db /var/lib/cwl/stg0faa6360-76d3-4dc5-b364-fc1aa0a46302/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b43875/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 01:14:56,602] [INFO] Task succeeded: Blastn
[2023-03-18 01:14:56,603] [INFO] Selected 31 target genomes.
[2023-03-18 01:14:56,603] [INFO] Target genome list was writen to OceanDNA-b43875/target_genomes_gtdb.txt
[2023-03-18 01:14:56,637] [INFO] Task started: fastANI
[2023-03-18 01:14:56,637] [INFO] Running command: fastANI --query /var/lib/cwl/stg6ca9acea-c106-4f70-ab38-5880f23705f0/OceanDNA-b43875.fa --refList OceanDNA-b43875/target_genomes_gtdb.txt --output OceanDNA-b43875/fastani_result_gtdb.tsv --threads 1
[2023-03-18 01:15:12,154] [INFO] Task succeeded: fastANI
[2023-03-18 01:15:12,155] [INFO] Found 0 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 01:15:12,155] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
--------------------------------------------------------------------------------
[2023-03-18 01:15:12,155] [INFO] GTDB search result was written to OceanDNA-b43875/result_gtdb.tsv
[2023-03-18 01:15:12,155] [INFO] ===== GTDB Search completed =====
[2023-03-18 01:15:12,156] [INFO] DFAST_QC result json was written to OceanDNA-b43875/dqc_result.json
[2023-03-18 01:15:12,156] [INFO] DFAST_QC completed!
[2023-03-18 01:15:12,156] [INFO] Total running time: 0h1m11s