[2023-03-17 08:39:27,374] [INFO] DFAST_QC pipeline started.
[2023-03-17 08:39:27,374] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 08:39:27,374] [INFO] DQC Reference Directory: /var/lib/cwl/stgdb3e6ba8-d14b-4359-a0e5-d8456f7e2d53/dqc_reference
[2023-03-17 08:39:28,534] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 08:39:28,534] [INFO] Task started: Prodigal
[2023-03-17 08:39:28,535] [INFO] Running command: cat /var/lib/cwl/stg6fd0395e-1d42-4089-9d5d-61a5c93e1a09/OceanDNA-b44346.fa | prodigal -d OceanDNA-b44346/cds.fna -a OceanDNA-b44346/protein.faa -g 11 -q > /dev/null
[2023-03-17 08:39:37,342] [INFO] Task succeeded: Prodigal
[2023-03-17 08:39:37,342] [INFO] Task started: HMMsearch
[2023-03-17 08:39:37,342] [INFO] Running command: hmmsearch --tblout OceanDNA-b44346/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgdb3e6ba8-d14b-4359-a0e5-d8456f7e2d53/dqc_reference/reference_markers.hmm OceanDNA-b44346/protein.faa > /dev/null
[2023-03-17 08:39:37,508] [INFO] Task succeeded: HMMsearch
[2023-03-17 08:39:37,509] [INFO] Found 6/6 markers.
[2023-03-17 08:39:37,521] [INFO] Query marker FASTA was written to OceanDNA-b44346/markers.fasta
[2023-03-17 08:39:37,521] [INFO] Task started: Blastn
[2023-03-17 08:39:37,521] [INFO] Running command: blastn -query OceanDNA-b44346/markers.fasta -db /var/lib/cwl/stgdb3e6ba8-d14b-4359-a0e5-d8456f7e2d53/dqc_reference/reference_markers.fasta -out OceanDNA-b44346/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 08:39:38,050] [INFO] Task succeeded: Blastn
[2023-03-17 08:39:38,051] [INFO] Selected 35 target genomes.
[2023-03-17 08:39:38,051] [INFO] Target genome list was writen to OceanDNA-b44346/target_genomes.txt
[2023-03-17 08:39:38,713] [INFO] Task started: fastANI
[2023-03-17 08:39:38,713] [INFO] Running command: fastANI --query /var/lib/cwl/stg6fd0395e-1d42-4089-9d5d-61a5c93e1a09/OceanDNA-b44346.fa --refList OceanDNA-b44346/target_genomes.txt --output OceanDNA-b44346/fastani_result.tsv --threads 1
[2023-03-17 08:39:58,402] [INFO] Task succeeded: fastANI
[2023-03-17 08:39:58,403] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgdb3e6ba8-d14b-4359-a0e5-d8456f7e2d53/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 08:39:58,403] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgdb3e6ba8-d14b-4359-a0e5-d8456f7e2d53/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 08:39:58,403] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 08:39:58,403] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 08:39:58,403] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 08:39:58,403] [INFO] DFAST Taxonomy check result was written to OceanDNA-b44346/tc_result.tsv
[2023-03-17 08:39:58,404] [INFO] ===== Taxonomy check completed =====
[2023-03-17 08:39:58,404] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 08:39:58,404] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgdb3e6ba8-d14b-4359-a0e5-d8456f7e2d53/dqc_reference/checkm_data
[2023-03-17 08:39:58,406] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 08:39:58,410] [INFO] Task started: CheckM
[2023-03-17 08:39:58,410] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b44346/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b44346/checkm_input OceanDNA-b44346/checkm_result
[2023-03-17 08:40:24,987] [INFO] Task succeeded: CheckM
[2023-03-17 08:40:24,988] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-17 08:40:24,990] [INFO] ===== Completeness check finished =====
[2023-03-17 08:40:24,990] [INFO] ===== Start GTDB Search =====
[2023-03-17 08:40:24,990] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b44346/markers.fasta)
[2023-03-17 08:40:24,991] [INFO] Task started: Blastn
[2023-03-17 08:40:24,991] [INFO] Running command: blastn -query OceanDNA-b44346/markers.fasta -db /var/lib/cwl/stgdb3e6ba8-d14b-4359-a0e5-d8456f7e2d53/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b44346/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 08:40:25,711] [INFO] Task succeeded: Blastn
[2023-03-17 08:40:25,712] [INFO] Selected 30 target genomes.
[2023-03-17 08:40:25,712] [INFO] Target genome list was writen to OceanDNA-b44346/target_genomes_gtdb.txt
[2023-03-17 08:40:26,330] [INFO] Task started: fastANI
[2023-03-17 08:40:26,330] [INFO] Running command: fastANI --query /var/lib/cwl/stg6fd0395e-1d42-4089-9d5d-61a5c93e1a09/OceanDNA-b44346.fa --refList OceanDNA-b44346/target_genomes_gtdb.txt --output OceanDNA-b44346/fastani_result_gtdb.tsv --threads 1
[2023-03-17 08:40:38,864] [INFO] Task succeeded: fastANI
[2023-03-17 08:40:38,866] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-03-17 08:40:38,866] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_018658305.1	s__Thiopontia sp018658305	99.8305	458	547	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__GCF-002020875;f__GCF-002020875;g__Thiopontia	95.0	99.91	99.80	0.92	0.89	16	conclusive
GCA_014384675.1	s__Thiopontia autotrophica	76.6587	67	547	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__GCF-002020875;f__GCF-002020875;g__Thiopontia	95.0	99.91	99.91	0.96	0.96	2	-
--------------------------------------------------------------------------------
[2023-03-17 08:40:38,866] [INFO] GTDB search result was written to OceanDNA-b44346/result_gtdb.tsv
[2023-03-17 08:40:38,867] [INFO] ===== GTDB Search completed =====
[2023-03-17 08:40:38,867] [INFO] DFAST_QC result json was written to OceanDNA-b44346/dqc_result.json
[2023-03-17 08:40:38,867] [INFO] DFAST_QC completed!
[2023-03-17 08:40:38,867] [INFO] Total running time: 0h1m11s