[2023-03-17 04:35:35,474] [INFO] DFAST_QC pipeline started.
[2023-03-17 04:35:35,474] [INFO] DFAST_QC version: 0.5.7
[2023-03-17 04:35:35,474] [INFO] DQC Reference Directory: /var/lib/cwl/stg1b9706ac-c158-4c53-9c3e-0c75af69f3a5/dqc_reference
[2023-03-17 04:35:36,687] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-17 04:35:36,687] [INFO] Task started: Prodigal
[2023-03-17 04:35:36,687] [INFO] Running command: cat /var/lib/cwl/stg4afba59b-ea5d-4ff5-9ec7-2d282ec5e22b/OceanDNA-b44367.fa | prodigal -d OceanDNA-b44367/cds.fna -a OceanDNA-b44367/protein.faa -g 11 -q > /dev/null
[2023-03-17 04:35:42,832] [INFO] Task succeeded: Prodigal
[2023-03-17 04:35:42,833] [INFO] Task started: HMMsearch
[2023-03-17 04:35:42,833] [INFO] Running command: hmmsearch --tblout OceanDNA-b44367/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1b9706ac-c158-4c53-9c3e-0c75af69f3a5/dqc_reference/reference_markers.hmm OceanDNA-b44367/protein.faa > /dev/null
[2023-03-17 04:35:42,999] [INFO] Task succeeded: HMMsearch
[2023-03-17 04:35:43,000] [INFO] Found 6/6 markers.
[2023-03-17 04:35:43,009] [INFO] Query marker FASTA was written to OceanDNA-b44367/markers.fasta
[2023-03-17 04:35:43,010] [INFO] Task started: Blastn
[2023-03-17 04:35:43,010] [INFO] Running command: blastn -query OceanDNA-b44367/markers.fasta -db /var/lib/cwl/stg1b9706ac-c158-4c53-9c3e-0c75af69f3a5/dqc_reference/reference_markers.fasta -out OceanDNA-b44367/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 04:35:43,540] [INFO] Task succeeded: Blastn
[2023-03-17 04:35:43,541] [INFO] Selected 26 target genomes.
[2023-03-17 04:35:43,541] [INFO] Target genome list was writen to OceanDNA-b44367/target_genomes.txt
[2023-03-17 04:35:43,553] [INFO] Task started: fastANI
[2023-03-17 04:35:43,553] [INFO] Running command: fastANI --query /var/lib/cwl/stg4afba59b-ea5d-4ff5-9ec7-2d282ec5e22b/OceanDNA-b44367.fa --refList OceanDNA-b44367/target_genomes.txt --output OceanDNA-b44367/fastani_result.tsv --threads 1
[2023-03-17 04:35:53,686] [INFO] Task succeeded: fastANI
[2023-03-17 04:35:53,687] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1b9706ac-c158-4c53-9c3e-0c75af69f3a5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-17 04:35:53,687] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1b9706ac-c158-4c53-9c3e-0c75af69f3a5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-17 04:35:53,687] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-03-17 04:35:53,687] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-03-17 04:35:53,687] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-03-17 04:35:53,687] [INFO] DFAST Taxonomy check result was written to OceanDNA-b44367/tc_result.tsv
[2023-03-17 04:35:53,687] [INFO] ===== Taxonomy check completed =====
[2023-03-17 04:35:53,687] [INFO] ===== Start completeness check using CheckM =====
[2023-03-17 04:35:53,688] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1b9706ac-c158-4c53-9c3e-0c75af69f3a5/dqc_reference/checkm_data
[2023-03-17 04:35:53,690] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-17 04:35:53,693] [INFO] Task started: CheckM
[2023-03-17 04:35:53,693] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b44367/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b44367/checkm_input OceanDNA-b44367/checkm_result
[2023-03-17 04:36:14,397] [INFO] Task succeeded: CheckM
[2023-03-17 04:36:14,398] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.67%
Contamintation: 0.38%
Strain heterogeneity: 100.00%
--------------------------------------------------------------------------------
[2023-03-17 04:36:14,399] [INFO] ===== Completeness check finished =====
[2023-03-17 04:36:14,400] [INFO] ===== Start GTDB Search =====
[2023-03-17 04:36:14,400] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b44367/markers.fasta)
[2023-03-17 04:36:14,400] [INFO] Task started: Blastn
[2023-03-17 04:36:14,400] [INFO] Running command: blastn -query OceanDNA-b44367/markers.fasta -db /var/lib/cwl/stg1b9706ac-c158-4c53-9c3e-0c75af69f3a5/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b44367/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-17 04:36:15,244] [INFO] Task succeeded: Blastn
[2023-03-17 04:36:15,245] [INFO] Selected 27 target genomes.
[2023-03-17 04:36:15,245] [INFO] Target genome list was writen to OceanDNA-b44367/target_genomes_gtdb.txt
[2023-03-17 04:36:15,761] [INFO] Task started: fastANI
[2023-03-17 04:36:15,761] [INFO] Running command: fastANI --query /var/lib/cwl/stg4afba59b-ea5d-4ff5-9ec7-2d282ec5e22b/OceanDNA-b44367.fa --refList OceanDNA-b44367/target_genomes_gtdb.txt --output OceanDNA-b44367/fastani_result_gtdb.tsv --threads 1
[2023-03-17 04:36:25,355] [INFO] Task succeeded: fastANI
[2023-03-17 04:36:25,358] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-17 04:36:25,358] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_002171615.1	s__TMED78 sp002171615	79.2787	233	417	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__UBA6522;f__UBA6522;g__TMED78	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-17 04:36:25,358] [INFO] GTDB search result was written to OceanDNA-b44367/result_gtdb.tsv
[2023-03-17 04:36:25,358] [INFO] ===== GTDB Search completed =====
[2023-03-17 04:36:25,358] [INFO] DFAST_QC result json was written to OceanDNA-b44367/dqc_result.json
[2023-03-17 04:36:25,359] [INFO] DFAST_QC completed!
[2023-03-17 04:36:25,359] [INFO] Total running time: 0h0m50s