[2023-03-18 09:15:00,470] [INFO] DFAST_QC pipeline started.
[2023-03-18 09:15:00,470] [INFO] DFAST_QC version: 0.5.7
[2023-03-18 09:15:00,470] [INFO] DQC Reference Directory: /var/lib/cwl/stg215fbb13-0bd7-4108-a4a0-e05931806e39/dqc_reference
[2023-03-18 09:15:02,033] [INFO] ===== Start taxonomy check using ANI =====
[2023-03-18 09:15:02,034] [INFO] Task started: Prodigal
[2023-03-18 09:15:02,034] [INFO] Running command: cat /var/lib/cwl/stgd32a53d4-1bec-45fc-95a4-13bc72c35587/OceanDNA-b44682.fa | prodigal -d OceanDNA-b44682/cds.fna -a OceanDNA-b44682/protein.faa -g 11 -q > /dev/null
[2023-03-18 09:15:17,258] [INFO] Task succeeded: Prodigal
[2023-03-18 09:15:17,258] [INFO] Task started: HMMsearch
[2023-03-18 09:15:17,258] [INFO] Running command: hmmsearch --tblout OceanDNA-b44682/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg215fbb13-0bd7-4108-a4a0-e05931806e39/dqc_reference/reference_markers.hmm OceanDNA-b44682/protein.faa > /dev/null
[2023-03-18 09:15:17,450] [INFO] Task succeeded: HMMsearch
[2023-03-18 09:15:17,450] [INFO] Found 6/6 markers.
[2023-03-18 09:15:17,484] [INFO] Query marker FASTA was written to OceanDNA-b44682/markers.fasta
[2023-03-18 09:15:17,484] [INFO] Task started: Blastn
[2023-03-18 09:15:17,484] [INFO] Running command: blastn -query OceanDNA-b44682/markers.fasta -db /var/lib/cwl/stg215fbb13-0bd7-4108-a4a0-e05931806e39/dqc_reference/reference_markers.fasta -out OceanDNA-b44682/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 09:15:18,284] [INFO] Task succeeded: Blastn
[2023-03-18 09:15:18,295] [INFO] Selected 10 target genomes.
[2023-03-18 09:15:18,295] [INFO] Target genome list was writen to OceanDNA-b44682/target_genomes.txt
[2023-03-18 09:15:18,301] [INFO] Task started: fastANI
[2023-03-18 09:15:18,301] [INFO] Running command: fastANI --query /var/lib/cwl/stgd32a53d4-1bec-45fc-95a4-13bc72c35587/OceanDNA-b44682.fa --refList OceanDNA-b44682/target_genomes.txt --output OceanDNA-b44682/fastani_result.tsv --threads 1
[2023-03-18 09:15:25,191] [INFO] Task succeeded: fastANI
[2023-03-18 09:15:25,192] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg215fbb13-0bd7-4108-a4a0-e05931806e39/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-03-18 09:15:25,192] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg215fbb13-0bd7-4108-a4a0-e05931806e39/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-03-18 09:15:25,199] [INFO] Found 10 fastANI hits (0 hits with ANI > threshold)
[2023-03-18 09:15:25,199] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-03-18 09:15:25,199] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Arenimonas soli	strain=CGMCC 1.15905	GCA_014643775.1	2269504	2269504	type	True	92.294	717	817	95	below_threshold
Arenimonas donghaensis	strain=HO3-R19	GCA_000743535.1	375061	375061	type	True	88.5143	690	817	95	below_threshold
Arenimonas malthae	strain=CC-JY-1	GCA_000747075.1	354197	354197	type	True	86.7936	611	817	95	below_threshold
Arenimonas caeni	strain=z29	GCA_003024235.1	2058085	2058085	type	True	86.706	640	817	95	below_threshold
Arenimonas metalli	strain=CF5-1	GCA_000747155.1	948077	948077	type	True	84.9204	619	817	95	below_threshold
Arenimonas terrae	strain=R29	GCA_006265115.1	2546226	2546226	type	True	84.5115	639	817	95	below_threshold
Arenimonas composti	strain=DSM 18010	GCA_000426365.1	370776	370776	type	True	82.04	563	817	95	below_threshold
Luteimonas lumbrici	strain=1.1416	GCA_006476065.1	2559601	2559601	type	True	79.735	371	817	95	below_threshold
Stenotrophomonas acidaminiphila	strain=DSM 13117	GCA_024221815.1	128780	128780	type	True	79.5726	388	817	95	below_threshold
Xanthomonas translucens	strain=ATCC 19319	GCA_020880735.1	343	343	type	True	79.06	366	817	95	below_threshold
--------------------------------------------------------------------------------
[2023-03-18 09:15:25,199] [INFO] DFAST Taxonomy check result was written to OceanDNA-b44682/tc_result.tsv
[2023-03-18 09:15:25,199] [INFO] ===== Taxonomy check completed =====
[2023-03-18 09:15:25,199] [INFO] ===== Start completeness check using CheckM =====
[2023-03-18 09:15:25,199] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg215fbb13-0bd7-4108-a4a0-e05931806e39/dqc_reference/checkm_data
[2023-03-18 09:15:25,200] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-03-18 09:15:25,204] [INFO] Task started: CheckM
[2023-03-18 09:15:25,204] [INFO] Running command: checkm taxonomy_wf --tab_table -f OceanDNA-b44682/cc_result.tsv -t 1 life "Prokaryote" OceanDNA-b44682/checkm_input OceanDNA-b44682/checkm_result
[2023-03-18 09:16:05,691] [INFO] Task succeeded: CheckM
[2023-03-18 09:16:05,691] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 93.06%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-03-18 09:16:05,694] [INFO] ===== Completeness check finished =====
[2023-03-18 09:16:05,694] [INFO] ===== Start GTDB Search =====
[2023-03-18 09:16:05,694] [INFO] Query marker FASTA already exists. Will reuse it. (OceanDNA-b44682/markers.fasta)
[2023-03-18 09:16:05,695] [INFO] Task started: Blastn
[2023-03-18 09:16:05,695] [INFO] Running command: blastn -query OceanDNA-b44682/markers.fasta -db /var/lib/cwl/stg215fbb13-0bd7-4108-a4a0-e05931806e39/dqc_reference/reference_markers_gtdb.fasta -out OceanDNA-b44682/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-03-18 09:16:07,137] [INFO] Task succeeded: Blastn
[2023-03-18 09:16:07,138] [INFO] Selected 9 target genomes.
[2023-03-18 09:16:07,138] [INFO] Target genome list was writen to OceanDNA-b44682/target_genomes_gtdb.txt
[2023-03-18 09:16:07,147] [INFO] Task started: fastANI
[2023-03-18 09:16:07,147] [INFO] Running command: fastANI --query /var/lib/cwl/stgd32a53d4-1bec-45fc-95a4-13bc72c35587/OceanDNA-b44682.fa --refList OceanDNA-b44682/target_genomes_gtdb.txt --output OceanDNA-b44682/fastani_result_gtdb.tsv --threads 1
[2023-03-18 09:16:13,180] [INFO] Task succeeded: fastANI
[2023-03-18 09:16:13,186] [INFO] Found 9 fastANI hits (0 hits with ANI > circumscription radius)
[2023-03-18 09:16:13,186] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014643775.1	s__Arenimonas soli	92.294	717	817	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Arenimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000743535.1	s__Arenimonas donghaensis	88.5143	690	817	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Arenimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000747075.1	s__Arenimonas malthae	86.7936	611	817	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Arenimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003024235.1	s__Arenimonas caeni	86.7252	639	817	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Arenimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_001724815.1	s__Arenimonas sp001724815	86.5801	628	817	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Arenimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003497545.1	s__Arenimonas sp003497545	85.805	497	817	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Arenimonas	95.0	99.43	99.43	0.81	0.81	2	-
GCF_000747155.1	s__Arenimonas metalli	84.9221	619	817	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Arenimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007993735.1	s__Arenimonas daejeonensis	84.498	595	817	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Arenimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003241895.1	s__Arenimonas sp003241895	83.7524	584	817	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Arenimonas	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-03-18 09:16:13,187] [INFO] GTDB search result was written to OceanDNA-b44682/result_gtdb.tsv
[2023-03-18 09:16:13,187] [INFO] ===== GTDB Search completed =====
[2023-03-18 09:16:13,188] [INFO] DFAST_QC result json was written to OceanDNA-b44682/dqc_result.json
[2023-03-18 09:16:13,188] [INFO] DFAST_QC completed!
[2023-03-18 09:16:13,188] [INFO] Total running time: 0h1m13s